Preparation method of Tlr7a-induced enhanced DC-NK cells

An enhanced technology of NK cells, applied in the field of biomedicine, can solve the problems of insignificant cytotoxic activity and achieve the effects of low production cost, increased killing activity, and easy control of conditions

Inactive Publication Date: 2016-11-23
SHENZHEN HORNETCORN BIOTECH
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the deficiencies of the existing cell technology, and provide a preparation method of Tlr7a (structural formula shown in the figure below) induced enhanced DC-NK cells and its Application, solves the problems of the prior art DC-NK cell culture, the improvement of cytotoxic activity is not significant, etc.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of Tlr7a-induced enhanced DC-NK cells
  • Preparation method of Tlr7a-induced enhanced DC-NK cells
  • Preparation method of Tlr7a-induced enhanced DC-NK cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] In Example 1, the obtained PBMCs were isolated and cultured. Include the following steps:

[0028] Collect 100ml of peripheral blood from the patient's venipuncture, transfer it to an anticoagulant tube containing sodium heparin, mix it evenly up and down, and obtain mononuclear cells through density gradient centrifugation of lymph separation fluid. The specific steps are: centrifuge the uniformly mixed whole blood at 1500 rpm for 10 minutes, absorb the upper layer liquid, that is, plasma, inactivate at 56°C for 30 minutes, centrifuge at 2500 rpm for 10 minutes, and set aside. Dilute the precipitated whole blood cells with normal saline 1:1, add the human lymphocyte separation medium and the diluted whole blood cell liquid into the centrifuge tube at a ratio of 1:2, centrifuge at 2000 rpm for 20 minutes, absorb the buffy coat layer, wash with normal saline Wash twice at 1600 rpm and 1300 rpm, respectively, and centrifuge for 7 minutes to obtain mononuclear cells.

[...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The present invention provides a method for preparing Tlr7a-induced enhanced DC-NK cells. In the technology of the present invention, DC-NK cells are highly active lymphocytes obtained by separating from peripheral blood of patients and adding a series of cytokines and Tlr7a stimulation to the in vitro culture system. Through this culture method, DC-NK cells are massively expanded and activated, and the tumor-killing ability of DC-NK cells is increased, reaching the level of clinical application in treating tumors. Compared with the DC-NK cells prepared by the method of the present invention and the DC-NK cells obtained by the common method, the NK cell killing effect obtained is significantly higher than that of the DC-NK cells cultured by the conventional method. The method can obtain a large number of DC-NK cells with safe application and stronger killing activity under in vitro culture conditions, and has the advantages of low preparation cost, simple process, easy control of conditions, low requirements for equipment, and easy large-scale production, etc. Advantage.

Description

technical field [0001] The invention belongs to the fields of biomedicine, cellular immunology and tumor immunotherapy, and relates to a preparation method of Tlr7a-inducible enhanced DC-NK cells and an application of anti-tumor adoptive immunotherapy. Background technique [0002] Tumor is a major disease that plagues human health, but due to its high complexity, diversity, variability and heterogeneity, an ideal treatment method has not been found. Scientists have invented adoptive immunotherapy for tumor patients who cannot independently produce more effective killing effector cells. Adoptive immunotherapy is a hotspot in the research of tumor treatment, and it has been widely used in clinic. [0003] NK cells or natural killer cells mainly express CD3 - CD56 + Phenotype is an important part of the natural immune system and the body's first line of defense against tumors and infections. Unlike T lymphocytes, NK cells can directly kill tumor cells and virus-infected ce...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0783C12N5/0784
CPCC12N5/0646C12N5/0639C12N2501/2301C12N2501/2302C12N2501/24C12N2501/50C12N2501/515
Inventor 高东李旺陈艳媛王宇环魏志璋罗晓玲
Owner SHENZHEN HORNETCORN BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap