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Filter membrane type sampling and culturing method for algal cells in ambient air

A technology of ambient air and algae cells, applied in the field of ambient air microbial monitoring, to achieve the effect of easy observation and separation, and pure samples

Inactive Publication Date: 2016-11-23
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a method for sampling and culturing algae cells in the air, so as to solve the problem of collecting algae cells in the air, and enable the collected algae cells to grow under laboratory conditions

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example1 Sampling with glass fiber filters and BG11 culture medium for algal cells

[0026] Take the glass fiber filter membrane and pack it with kraft paper, and carry out autoclaving at 121°C for 30min. Disassemble the filter clip of the air particle sampler, use 95% ethanol aqueous solution to wipe and disinfect the components of the filter clip, wipe it 3 times, take out the sterilized glass fiber filter membrane, and put it into the sterilized sampling clip . Turn on the medium-flow air particle sampler, the airflow sampling speed is 80L / min, and the collection time is 4 hours. The preparation of BG11 culture is based on a 500mL transparent glass conical flask, the pH of the medium is adjusted to 7.1-7.2, and autoclaved at 121°C for 30min. Take out the sampled glass fiber filter membrane and immediately put it into the Erlenmeyer flask filled with BG11 medium. The Erlenmeyer flask was placed in a light incubator, the culture temperature was 25°C, the day...

Embodiment 2

[0027] Example 2 Sampling with glass fiber filters and BB culture medium for algal cells

[0028] Take the glass fiber filter membrane and pack it with kraft paper, and carry out autoclaving at 121°C for 30min. Disassemble the filter clip of the air particle sampler, use 95% ethanol aqueous solution to wipe and disinfect the components of the filter clip, wipe it 3 times, take out the sterilized glass fiber filter membrane, and put it into the sterilized sampling clip . Turn on the medium-flow air particle sampler, the airflow sampling speed is 70L / min, and the collection time is 3 hours. Prepare BB culture in a 500mL transparent glass Erlenmeyer flask, adjust the pH value of the medium to 7.1-7.2, and sterilize under high pressure at 121°C for 30min. Take out the sampled glass fiber filter membrane and put it into the Erlenmeyer flask filled with BB medium immediately. The Erlenmeyer flask was placed in a light incubator, the culture temperature was 30°C, the day:...

Embodiment 3

[0029] Example 3 Sampling using polyvinyl chloride filters and BB culture medium for algal cells

[0030] Take the polyvinyl chloride filter membrane and pack it with kraft paper, and carry out high-pressure sterilization at 121°C for 30 minutes. Disassemble the filter clip of the air particle sampler, use 85% ethanol aqueous solution to wipe and disinfect the components of the filter clip, wipe it 3 times, take out the sterilized polyvinyl chloride filter membrane, and put it into the sterilized sampling clip middle. Turn on the medium-flow air particle sampler, the airflow sampling speed is 80L / min, and the collection time is 6 hours. Prepare BB culture in a 500mL transparent glass Erlenmeyer flask, adjust the pH value of the medium to 7.1-7.2, and sterilize under high pressure at 121°C for 30min. Take out the polyvinyl chloride filter membrane after sampling, and put it into the Erlenmeyer flask filled with BB medium immediately. The Erlenmeyer flask was placed ...

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PUM

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Abstract

The invention relates to the field of monitoring for microorganisms in ambient air, in particular to a filter membrane type sampling and culturing method for algal cells in ambient air. The method comprises following steps: 1), sterilizing a sampling filter membrane and a culture solution; 2), disinfecting a filter membrane sampling clamp; 3), collecting the algal cells in the air; 4), culturing the algal cells in the air through the sampling filter membrane to obtain samples of the algal cells in the air. The method has the advantages that the algal cells in the air can be effectively collected with the method, and the collected algal cells can be cultured and grown under laboratory conditions.

Description

technical field [0001] The invention relates to the field of ambient air microbial monitoring, in particular to a method for sampling and cultivating algal cell membranes in ambient air. Background technique [0002] In recent years, the issue of ambient air pollution has attracted much attention. Generally, ambient air pollution is divided into physical and chemical pollution and microbial pollution. At present, the focus of attention on ambient air pollution is still physical and chemical pollution, such as: smoke, dust, TSP, PM 10 , PM 2.5 , SO 2 , NO x , photochemical smog, etc., less attention has been paid to the problems of microorganisms, and even less is known about algal cells in the air. [0003] From the perspective of microorganisms themselves, microorganisms in the air are ubiquitous. Microorganisms in the air will quickly lose their activity or die under strong light, but when they encounter suitable living conditions, they will multiply in large numbers,...

Claims

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Application Information

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IPC IPC(8): C12Q1/04C12N1/12C12R1/89
Inventor 梁文艳王媛媛胡静茹
Owner BEIJING FORESTRY UNIVERSITY