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A biotechnology method for improving the aroma quality of reconstituted tobacco leaves

A technology of reconstituted tobacco leaves and biotechnology, applied in the field of tobacco industry, to improve the quality of aroma, reduce irritation, and reduce green miscellaneous gas

Active Publication Date: 2017-10-17
HUBEI CHINA TOBACCO IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Using butyric acid bacteria ferulic acid esterase, lignin degrading enzyme synergistic effect, enterobactermori (Enterobactermori) VL4-3 compound biotransformation reconstituted tobacco aqueous solution, decompose and transform macromolecules such as lignin, cellulose and protein in reconstituted tobacco leaves The active substances are sugars, ferulic acid, etc., and then converted into ferulic acid and other flavor precursors such as vanillin, so that the reconstituted tobacco leaves produced can reduce irritation, reduce green miscellaneous gas, rich and beautiful aroma, and have a milk-sweet aroma. Improving the aroma quality of reconstituted tobacco leaves has not been reported

Method used

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  • A biotechnology method for improving the aroma quality of reconstituted tobacco leaves

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Experimental program
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Effect test

Embodiment 1

[0020] The present invention provides a kind of reconstituted tobacco leaf whose aroma quality is improved by biotechnology, comprising the following steps:

[0021] (1) Strain activation: Inoculate Enterobacter mori (Enterobacter mori) VL4-3 on the potato dextrose agar slant medium, and culture it statically for 28 hours at 27°C and pH 5 to obtain the slant culture, and then inoculate it into the seed culture base, shake culture for 12 hours at 28°C, rotation speed 100rpm / min, and prepare seed culture solution after transfer 1;

[0022] The Enterobacter mori VL4-3, whose strain preservation number is CCTCC NO: M2012020, was preserved in the Chinese Type Culture Collection Center of Wuhan University on February 16, 2012.

[0023] The slant medium: 200 mL / L of potato infusion, 20 g / L of glucose, and 13 g / L of agar. Described seed medium: maltose 50g / L, peptone 10g / L, sodium chloride 5g / L.

[0024](2) Composite biotransformation pretreatment: in the extraction process of recon...

Embodiment 2

[0027] The present invention provides a kind of reconstituted tobacco leaf whose aroma quality is improved by biotechnology, comprising the following steps:

[0028] (1) Strain activation: inoculate Enterobacter mulberry on the potato dextrose agar slant medium, under the conditions of 30°C and pH5.5, obtain the slant culture after static culture for 48 hours, then inoculate it into the seed medium, and inoculate it at 30°C , under the condition of rotating speed 110rpm / min, shake culture for 24h, and make seed culture solution after 2 consecutive transfers;

[0029] The deposit number of the Enterobacter mori VL4-3 is CCTCC NO: M2012020, which was deposited in the Chinese Center for Type Culture Collection of Wuhan University on February 16, 2012.

[0030] The slant medium: 200 mL / L of potato infusion, 20 g / L of glucose, and 13 g / L of agar. Described seed medium: maltose 50g / L, peptone 10g / L, sodium chloride 5g / L.

[0031] (2) Composite biotransformation pretreatment: In th...

Embodiment 3

[0034] The invention provides a method for improving the aroma quality of reconstituted tobacco leaves through biotechnology, comprising the following steps:

[0035] (1) Strain activation: inoculate Enterobacter mori (Enterobacter mori) VL4-3 into

[0036] On the potato dextrose agar slant medium, under the conditions of 32°C and pH6.5, the slant culture was obtained after static culture for 60 hours, and then inoculated into the seed medium, shaken at 32°C and 120rpm / min for 32h, continuous The seed culture solution was prepared after three transfers;

[0037] The deposit number of the Enterobacter mori VL4-3 is CCTCC NO: M2012020, which was deposited in the Chinese Center for Type Culture Collection of Wuhan University on February 16, 2012.

[0038] The slant medium: 200 mL / L of potato infusion, 20 g / L of glucose, and 13 g / L of agar. Described seed medium: maltose 50g / L, peptone 10g / L, sodium chloride 5g / L.

[0039] (2) Composite biotransformation pretreatment: in the ex...

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Abstract

The invention provides a method for improving fragrance quality of reconstituted tobacco by biotechnology. The method comprises the following steps: (1) strain activation: slant culture medium is inoculated with Enterobacter mori VL4-3 to obtain a slant culture, then a seed culture medium is inoculated with the slant culture, and transformation is conducted to obtain a seed culture solution; (2) complex biotransformation pretreatment: in the reconstituted tobacco extraction process, a reconstituted tobacco aqueous solution is inoculated with the seed culture solution, a compound enzyme of clostridium butylicum feruloyl esterase, lignin peroxidase (Lip) and manganese peroxidase (MnP) is added, uniform stirring and mixing are performed, and fermentation is conducted to obtain a reconstituted tobacco biotransformation solution; (3) reconstituted tobacco is produced through a conventional process of reconstituted tobacco, wherein in the process, the temperature exceeds 75 DEG C, enzymes and the bacteria are inactivated, and rolling is conducted to obtain the reconstituted tobacco. According to the reconstituted tobacco, of which the fragrance quality is improved by the biotechnology, produced by the method, the irritation is reduced, the green miscellaneous gas is reduced, the fragrance is rich and elegant and is sweet as milk, and the fragrance quality of the reconstituted tobacco is improved.

Description

technical field [0001] The invention relates to the field of tobacco industry, in particular to a method for improving the aroma quality of reconstituted tobacco leaves through biotechnology. Background technique [0002] Reconstituted tobacco leaves are mainly composed of tobacco powder, chips, tobacco stems or low-grade tobacco leaves with adhesives and other additives. Tobacco dust, chips, and tobacco stems are by-products of the tobacco industry, and were mostly disposed of in the past. my country is a big tobacco country, and hundreds of thousands of tons of tobacco dust, debris, and tobacco stem resources are discarded every year, which not only causes environmental pollution, but also wastes natural resources. Studies have shown that the types of ingredients contained in tobacco powder, chips, and tobacco stems are basically the same as those of tobacco leaves, but there are flavor defects such as heavy miscellaneous gas, strong irritation, and unpleasant taste. [...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A24B3/14A24B15/20
CPCA24B3/14A24B15/20
Inventor 陈义坤罗诚浩高颂魏敏李冉柯炜昌
Owner HUBEI CHINA TOBACCO IND
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