Method for improving sensory quality of reconstituted tobaccos through biotransformation

A technology of reconstituted tobacco leaves and biotransformation, applied in the field of tobacco industry, can solve the problems of reducing irritation, achieve the effects of reducing irritation, improving sensory quality, and reducing green miscellaneous gas

Active Publication Date: 2017-01-04
HUBEI CHINA TOBACCO IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The aqueous solution of reconstituted tobacco leaves was used as the substrate, and Penicillium citrinum ferulic acid esterase, xylanase, glucose oxidase, catalase, and phenol oxidase were used to act synergistically, and Bacillus methylotrophicus (VJ4-1 ) transformation, decomposes lignin, cellulose, protein and other macromolecular irritating substances in reconstituted tobacco leaves, releases ferulic acid, and converts ferulic acid into flavor precursors such as vanillin, so that the produced reconstituted tobacco leaves reduce irritation, Reduce green miscellaneous gas, beautiful aroma, slightly milky aroma, improve the sensory quality of reconstituted tobacco leaves, which has not been reported yet

Method used

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  • Method for improving sensory quality of reconstituted tobaccos through biotransformation

Examples

Experimental program
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Embodiment 1

[0020] A method for biotransforming reconstituted tobacco leaves for improving organoleptic quality, comprising the following steps:

[0021] (1) Strain activation: Bacillus methylotrophicus (VJ4-1) was inoculated on the potato dextrose agar slant medium, and the slant culture was obtained after static culture at 28°C and pH 5 for 28 hours, and then inoculated into the seeds The culture medium was shaken for 12 hours at 28°C with a rotational speed of 115rpm / min, and the seed culture solution was obtained after two consecutive transfers;

[0022] The slant medium: 200 mL / L of potato infusion, 20 g / L of glucose, and 13 g / L of agar. Described seed medium: maltose 50g / L, peptone 10g / L, sodium chloride 5g / L.

[0023] (2) Composite biotransformation pretreatment: in the extraction process of reconstituted tobacco leaves, the seed culture solution described in step 1) is inoculated into the reconstituted tobacco aqueous solution at a mass ratio of 5%, and 0.18% of the mass ratio of...

Embodiment 2

[0026] A method for biotransforming reconstituted tobacco leaves for improving organoleptic quality, comprising the following steps:

[0027] (1) Strain activation: Bacillus methylotrophicus (VJ4-1) was inoculated on the potato dextrose agar slant medium, and the slant culture was obtained after static culture at 30°C and pH5.5 for 36 hours, and then inoculated Transfer to the seed medium, shake and cultivate at 30°C and 120rpm / min for 18 hours, and transfer 3 times in succession to obtain the seed culture solution;

[0028] The slant medium: 200 mL / L of potato infusion, 20 g / L of glucose, and 13 g / L of agar. Described seed medium: maltose 50g / L, peptone 10g / L, sodium chloride 5g / L.

[0029] (2) Composite biotransformation pretreatment: in the extraction process of reconstituted tobacco leaves, the seed culture solution described in step 1) is inoculated into the reconstituted tobacco leaf aqueous solution by mass ratio of 9%, and 0.3% of the mass ratio of reconstituted tobacco...

Embodiment 3

[0032] A method for biotransforming reconstituted tobacco leaves for improving organoleptic quality, comprising the following steps:

[0033] (1) Strain activation: Bacillus methylotrophicus (VJ4-1) was inoculated on the potato dextrose agar slant medium, and the slant culture was obtained after static culture at 32°C and pH 6 for 72 hours, and then inoculated into the seeds The culture medium was shaken for 30 hours at 32°C with a rotational speed of 125rpm / min, and the seed culture solution was obtained after two consecutive transfers;

[0034] The slant medium: 200 mL / L of potato infusion, 20 g / L of glucose, and 13 g / L of agar. Described seed medium: maltose 50g / L, peptone 10g / L, sodium chloride 5g / L.

[0035] (2) Composite biotransformation pretreatment: in the extraction process of reconstituted tobacco leaves, the seed culture solution described in step 1) is inoculated into the reconstituted tobacco leaf aqueous solution by mass ratio of 12%, and 0.25% of the mass rati...

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Abstract

The invention provides a method for improving the sensory quality of reconstituted tobaccos through biotransformation. The method comprises the following steps that 1, strain activation is performed: bacillus methylotrophicus (VJ4-1) is inoculated to an oblique-plane culture medium and then is inoculated to a seed culture medium, and a seed culture solution is prepared through transfer; 2, composite biotransformation pretreatment is performed: in a reconstituted tobacco extraction process, the seed culture medium in the step 1 is inoculated to a reconstituted tobacco water solution, penicillium citrinum feruloyl esterase, xylanase, glucose oxidase, catalase, phenol oxidase and other complex enzymes are added, stirring and even mixing are performed, and a reconstituted tobacco biotransformation solution is obtained through fermentation; 3, the reconstituted tobaccos are produced through a conventional reconstituted tobacco process, the temperature exceed 75 DEG C in the process, the enzymes and bacteria are inactivated, and the reconstituted tobaccos are obtained through rolling. The produced reconstituted tobaccos with the aroma quality improved by adopting a biotechnology have good aroma with a slight milk flavor, the irritation is reduced, green miscellaneous smell is decreased, and the sensory quality of the reconstituted tobaccos is improved.

Description

technical field [0001] The invention relates to the field of tobacco industry, in particular to a method for improving the sensory quality of reconstituted tobacco leaves through biotransformation. Background technique [0002] Reconstituted tobacco leaves are mainly composed of tobacco powder, chips, tobacco stems or low-grade tobacco leaves with adhesives and other additives. Tobacco dust, chips, and tobacco stems are by-products of the tobacco industry, and were mostly disposed of in the past. my country is a big tobacco country, and hundreds of thousands of tons of tobacco dust, debris, and tobacco stem resources are discarded every year, which not only causes environmental pollution, but also wastes natural resources. Studies have shown that the types of ingredients contained in tobacco powder, chips, and tobacco stems are basically the same as those of tobacco leaves, but there are flavor defects such as heavy miscellaneous gas, strong irritation, and unpleasant taste...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A24B15/20
CPCA24B15/20
Inventor 罗诚浩陈义坤陈国平魏敏李超李冉
Owner HUBEI CHINA TOBACCO IND
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