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Preparation method of micron-grade superparamagnetic beads with spacer arms

A spacer arm, superparamagnetic technology, applied in the direction of magnetic materials, magnetic objects, inorganic materials, etc., can solve the problems of reducing the loading capacity and biological activity of biological macromolecules on the surface of magnetic beads, insufficient loading capacity, steric hindrance, etc. Achieve the effect of reducing non-specific interactions, improving activity and utilization, and expanding range

Inactive Publication Date: 2016-12-07
BIOTOOL LLC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the surface of magnetic beads is used as the carrier to couple biomacromolecules, its spatial structure will cause a certain steric hindrance, thereby reducing the loading amount and biological activity of biomacromolecules on the surface of magnetic beads. Micron-sized magnetic beads currently in the market There is a problem of insufficient loading

Method used

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  • Preparation method of micron-grade superparamagnetic beads with spacer arms
  • Preparation method of micron-grade superparamagnetic beads with spacer arms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A method for preparing micron-scale superparamagnetic beads with spacer arms, the specific steps are as follows:

[0017] Step 1, superparamagnetic Fe 3 o 4 Preparation of Micron Magnetic Beads: Synthesis of Superparamagnetic Fe by Co-precipitation Method 3 o 4 Micron magnetic beads: FeCl 3 ·6H 2 O and FeSO 4 ·7H 2 O was respectively dissolved in HCl solution with a concentration of 1 mol / L. The two solutions were mechanically stirred and mixed, protected by nitrogen gas, and ammonia water was added to make the pH of the solution reach 11. Stir vigorously at room temperature for 20 minutes, and use NdFe Boron magnets were used for magnetic separation, washed with high-purity water and absolute ethanol three times respectively, and vacuum-dried at 50°C for 14 hours;

[0018] Step 2, surface activation of magnetic beads: use the micron magnetic beads in step 1 to measure the hydroxyl density of the microspheres by potentiometric titration, and add 1-(3-dimethylamin...

Embodiment 2

[0021] A method for preparing micron-scale superparamagnetic beads with spacer arms, the specific steps are as follows:

[0022] Step 1, superparamagnetic Fe 3 o 4 Preparation of Micron Magnetic Beads: Synthesis of Superparamagnetic Fe by Co-precipitation Method 3 o 4 Micron magnetic beads: FeCl 3 ·6H 2 O and FeSO 4 ·7H 2 O was respectively dissolved in HCl solution with a concentration of 1 mol / L. The two solutions were mechanically stirred and mixed, protected by nitrogen gas, and ammonia water was added to make the pH of the solution reach 11. Stir vigorously at room temperature for 25 minutes. Boron magnets were used for magnetic separation, washed with high-purity water and absolute ethanol for 4 times, and vacuum-dried at 55°C for 10 hours;

[0023] Step 2, surface activation of magnetic beads: use the micron magnetic beads in step 1 to measure the hydroxyl density of the microspheres by potentiometric titration, and add 1-(3-dimethylaminopropyl) equivalent to the...

Embodiment 3

[0026] A method for preparing micron-scale superparamagnetic beads with spacer arms, the specific steps are as follows:

[0027] Step 1, superparamagnetic Fe 3 o 4 Preparation of Micron Magnetic Beads: Synthesis of Superparamagnetic Fe by Co-precipitation Method 3 o 4 Micron magnetic beads: FeCl 3 ·6H 2 O and FeSO 4 ·7H 2 O was respectively dissolved in HCl solution with a concentration of 1 mol / L. The two solutions were mechanically stirred and mixed, protected by nitrogen gas, and ammonia water was added to make the pH of the solution reach 11. Stir vigorously at room temperature for 30 minutes. Boron magnets are used for magnetic separation, washed with high-purity water and absolute ethanol for 5 times, and vacuum-dried at 60°C for 10-14 hours;

[0028] Step 2, surface activation of magnetic beads: use the micron magnetic beads in step 1 to measure the hydroxyl density of the microspheres by potentiometric titration, and add 1-(3-dimethylaminopropyl) equivalent to t...

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Abstract

The invention discloses a preparation method of micron-grade superparamagnetic beads with spacer arms. The preparation method specifically comprises the following steps: 1, preparation of superparamagnetic Fe3O4 micron beads; 2, surface activation of the beads; and 3, coupling of spacer arm molecules. According to the preparation process, spacer arms are introduced to the surfaces of the nano microspheres, thus reducing the steric hindrance of the coupled macromolecules, improving the activity and utilization ratio of the macromolecules and reducing the non-specific interaction on the surfaces of the macromolecules and beads; controllable preparation of the beads with different particle sizes within a certain range (2-5 micrometers) can be realized by proportioning and setting; instruments and devices used for the preparation process are simple and economic; the beads are adjustable in particle size, favorable in dispersivity and less prone to accumulation; the surface hydroxyl density can be adjusted to adjust the length and surface density of the spacer arms; and the tail end of each spacer arm is a carboxyl group and can react with a molecule containing an amino group, hydroxyl group or the like, thus overcoming the defect of low bead reaction activity, widening the scope of the micron bead and achieving favorable use effect.

Description

technical field [0001] The invention relates to a preparation method of magnetic beads, in particular to a preparation method of micron-sized superparamagnetic magnetic beads with spacer arms. Background technique [0002] Due to their superparamagnetic properties, micron-sized magnetic beads can only show conditional magnetism under the action of an external magnetic field, and have regular shape, small size, large specific surface area, and surface can be modified in various ways. They are widely used in biological reagents and medical supplies, etc. The field has a wide range of applications, especially in high-throughput biological sciences and other technological frontiers with obvious technical advantages. However, biological macromolecules such as proteins have a large molecular weight and will have a certain spatial structure, so they all have a large molecular volume. When the surface of magnetic beads is used as the carrier to couple biomacromolecules, its spatial...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01G49/08H01F1/36
CPCC01G49/08C01P2004/52C01P2004/61C01P2006/42H01F1/344H01F1/36
Inventor 张存清金希盛
Owner BIOTOOL LLC
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