Method of screening algicidal bacteria from soil in roots of Taihu lake reed and measuring algae-lytic effect of the algicidal bacteria

A technology of algae-lysing bacteria and algae-lysing bacteria, which is applied in the fields of botanical equipment and methods, bacteria, herbicides and algicides, etc., can solve the problems of destructive aquatic organisms poisoning, a lot of human and material resources, etc., and achieve a remarkable algae-dissolving effect. Effect

Inactive Publication Date: 2016-12-07
CHANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Physical methods include flocculation, manual salvage, air flotation algae removal, ultrasonic, etc. This method is simple to operate and has quick results, with less secondary pollution, but it cannot solve the problem from the root cause and requires a lot of manpower and material resources; the chemical method is through adding copper salt, high Potassium manganate and other chemical reagents can directly kill or inhibit the effect, but the strong lethality of chemical reagents will also poison the original aquatic organisms; biological methods control algae through biological chains or race competition. Including planting higher aquatic plants, cultivating aquatic animals and zooplankton that devour cyanobacteria, and adding efficient algae-dissolving bacteria

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Pick a ring of bacteria from the bacterial slant culture medium and insert it into 100mL of sterilized fresh bacterial liquid medium, and shake it in an incubator with a temperature of 30°C and a rotation speed of 130r / min for 18h logarithmic phase. The ratio of bacteria to algae is 1:2, that is, 50mL of bacteria liquid is inoculated into 100mL of algae liquid, and the initial chlorophyll a of the algae liquid is 94.5mg / m 3 , cultured in a light incubator with a temperature of 27°C, a light intensity of 2500 lux, and a light-dark cycle of 12h:12h, and shaken manually, and the chlorophyll content was measured every day until the content was basically unchanged, and the removal rate was 41.5%.

Embodiment 2

[0031] The difference between this embodiment and embodiment 1 is that the ratio of bacteria to algae is different.

[0032] Pick a ring of bacteria from the bacterial slant culture medium and insert it into 100mL of sterilized fresh bacterial liquid medium, and shake it in an incubator with a temperature of 30°C and a rotation speed of 130r / min for 18h logarithmic phase. The ratio of bacteria to algae is 1:10, that is, 10mL of bacteria liquid is inoculated into 100mL of algae liquid, and the initial chlorophyll a of the algae liquid is 94.5mg / m 3 , cultured in a light incubator with a temperature of 27°C, a light intensity of 2500 lux, and a light-dark cycle of 12h:12h, and shaken manually, and the chlorophyll content was measured every day until the content was basically unchanged, and the removal rate was 41.5%.

Embodiment 3

[0034] The difference between this example and example 1 and example 2 lies in the initial concentration, the light cycle is different, and the ratio of selected bacteria to algae is 1:10.

[0035] Pick a ring of bacteria from the bacterial slant culture medium and insert it into 100mL of sterilized fresh bacterial liquid medium, and shake it in an incubator with a temperature of 30°C and a rotation speed of 130r / min for 18h logarithmic phase. The ratio of bacteria to algae is 1:10, that is, 10mL of bacteria liquid is inoculated into 100mL of algae liquid, and the initial chlorophyll a of the algae liquid is 93.02mg / m 3, cultivated in a light incubator with a temperature of 27°C, a light intensity of 2500 lux, and a light and dark cycle of 24h:0h, 12h:12h, and 0h:24h, and shake it manually, measure the chlorophyll content every day until the content is basically unchanged, remove The rates are 44.1%, 52.2%, and 39.6%, respectively, so when the light-dark cycle is 12h:12h, the ...

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Abstract

The invention provides a method of screening algicidal bacteria from soil in roots of Taihu lake reed and measuring algae-lytic effect of the algicidal bacteria and belongs to the field of microbiology of cyanobacteria treatment. The invention provides a method of dissolving microcystis aeruginosa with the algicidal bacteria. In the method, a bacterial strain is separated and purified from the soil in roots of Taihu lake reed, and with the microcystis aeruginosa as an experiment raw material, a algicidal bacterial strain G6 is obtained. Under optimized conditions, the algicidal bacterial strain G6 reaches 82% in treatment effects on the microcystis aeruginosa and has significant algae-lytic effect. The soil in roots of Taihu lake reed can serve as a new source of the algicidal bacterial strain.

Description

technical field [0001] The invention belongs to the microbiological field of cyanobacteria treatment, and relates to a method for screening algae-dissolving bacteria from reed root soil in Taihu Lake and measuring the algae-dissolving effect thereof. Background technique [0002] With the acceleration of urbanization and the rapid development of industry and agriculture, a large amount of domestic sewage containing nitrogen, phosphorus, and potassium, as well as industrial and agricultural wastewater are discharged into rivers and lakes, resulting in serious eutrophication of water bodies and large-scale blue-green algae outbreaks. Cyanobacteria will not only destroy the ecological balance of the water body, consume dissolved oxygen in the water, cause the death of aquatic organisms, but also secrete algae toxins, substances that can threaten human health. [0003] The methods of algae control and algae removal are generally divided into physical methods, chemical methods an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/02C12N1/20A01N63/00A01P13/00
CPCC12N1/02A01N63/00C12N1/20
Inventor 张安妮沈红池齐今笛马昌明张文艺
Owner CHANGZHOU UNIV
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