Method for preparing oligosaccharide-containing seaweed organic fertilizers by graded enzymolysis

A technology of organic fertilizer and enzymatic hydrolysis, applied in the direction of organic fertilizer, nitrogen fertilizer, phosphate fertilizer, etc., can solve the problems of low content of effective substances, unclear enzymatic hydrolysis products, low enzyme production efficiency, etc., to increase content, shorten reaction time, improve The effect of reaction efficiency

Active Publication Date: 2017-01-04
WUZHOUFENG AGRI SCI & TECH
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

At present, there are few studies on the production of seaweed fertilizers by enzymatic hydrolysis in China. The only few studies generally have problems such as low enzyme production efficiency, unclear enzymatic hydrolysis products, and low content of active substances, which greatly affect the quality of the product.

Method used

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  • Method for preparing oligosaccharide-containing seaweed organic fertilizers by graded enzymolysis
  • Method for preparing oligosaccharide-containing seaweed organic fertilizers by graded enzymolysis
  • Method for preparing oligosaccharide-containing seaweed organic fertilizers by graded enzymolysis

Examples

Experimental program
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Effect test

Embodiment 1

[0031] 1) Preparation of Product A: Wash and remove fresh copper algae and kelp (mass ratio: 1:1), soak for 24 hours, refine, transfer to a fermenter, sterilize at 115°C for 30 minutes, and cool to 20 ℃ to get the product A, set aside.

[0032] 2) Preparation of enzyme solution: respectively activate Bacillus cereus and Bacillus megaterium and inoculate them into the same seed fermenter for mixed enrichment culture, the inoculum size is 10%, the temperature is 37°C, cultivate for 15 hours, and centrifuge the fermentation broth Cellulolytic enzyme solution A is obtained by membrane filtration to remove the bacteria; activate Bacillus saccharophilus and transfer to a seed fermentation tank for enrichment culture, the inoculum size is 10%, the temperature is 30°C, cultured for 12 hours, and the fermented liquid is centrifuged The bacterial cells were removed by membrane filtration to obtain alginic acid degrading enzyme solution B.

[0033] The inoculum size refers to the mass p...

Embodiment 2

[0041] 1) Preparation of product A: Wash fresh Sargassum and Sargassum (mass ratio 1:1), remove impurities, soak for 36 hours, grind, transfer to a fermenter, sterilize at 121°C for 30 minutes, and cool To 30 ℃ to obtain product A, set aside.

[0042] 2) Preparation of enzyme solution: activate Bacillus cereus and Bacillus megaterium and inoculate them into the same seed fermenter for mixed enrichment culture, the inoculum size is 15%, the temperature is 37°C, cultivate for 18 hours, centrifuge the fermentation broth Cellulolytic enzyme solution A is obtained by membrane filtration to remove the bacteria; activate Bacillus saccharophilus and transfer to a seed fermentation tank for enrichment culture, the inoculum size is 15%, and the temperature is 35 ° C, cultured for 14 hours, and the fermentation liquid is centrifuged The bacterial cells were removed by membrane filtration to obtain alginic acid degrading enzyme solution B.

[0043] The inoculum size refers to the mass pe...

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Abstract

The invention relates to a method for preparing oligosaccharide-containing seaweed organic fertilizers by graded enzymolysis. The method comprises the following steps: washing fresh marine algae plants, removing impurities, soaking, grinding into thick liquid, preparing an enzyme solution, and adding the enzyme solution into a seaweed base material at different times to realize graded enzymolysis. An experiment shows that the technical effect of graded enzymolysis by various enzymes is obviously better than the technical effect of single enzymolysis and the technical effect of a method of simply mixing the various enzymes for enzymolysis, reaction time is short, production efficiency is greatly improved, the contents of active ingredients such as alginic acid and oligosaccharide are high, and the whole process is high in controllability, and is energy-saving and environmentally friendly. The contents of the alginic acid and the brown seaweed oligosaccharide of the prepared oligosaccharide-containing seaweed organic fertilizers are above 20 g/L, pH value is 7.0-8.5, and the ratio of contents of N, P and K is 1-2:1:0.8-1.5.

Description

technical field [0001] The invention belongs to the field of agricultural fertilizers, and in particular relates to a method for preparing oligosaccharide-containing seaweed organic fertilizer by enzymatic hydrolysis. Background technique [0002] At present, the production methods for seaweed organic fertilizer mainly include chemical method, physical method and biological method. The chemical method is mainly to treat seaweed cells with acid, alkali and organic solvents, so that the cells can digest and release endogenous substances. This method is simple to operate and easy to implement, and it is also the method adopted by most seaweed fertilizer manufacturers at home and abroad. However, the damage of chemical reagents to the active substance components in seaweed cells is considerable, and the residual organic reagents are also potential hazards to the environment. The principle of the physical method is to control the physical environmental conditions such as pressur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G1/06C05F11/00
CPCC05B7/00C05F11/00C05C5/02C05C9/00
Inventor 王学江张志凯
Owner WUZHOUFENG AGRI SCI & TECH
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