Method for detecting development toxicity of environmental pollutants by whole-mount in-situ hybridization technique

Abstract

The invention belongs to the technical field of biology, and particularly relates to a method for detecting development toxicity of environmental pollutants by a whole-mount in-situ hybridization technique. The method comprises the following step: detecting the development toxicity of environmental pollutants to zebra fish embryos by a whole-mount in-situ hybridization technique. The method can be used for predicting potential toxic actions of the environmental pollutants on zebra fish organs and tissues, thereby further disclosing the action mode of the environmental pollutants, shortening the detection period, and achieving the goal of early warning in deed.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for detecting the developmental toxicity of environmental pollutants by using the overall in situ hybridization technique. Background technique [0002] Zebrafish is an important model organism, and its gene similarity with human genes is as high as about 87%. The experimental results obtained with zebrafish as experimental materials have great reference significance for the human body; therefore, zebrafish is very useful in toxicology research. Widely, especially zebrafish embryos are highly toxically sensitive to chemicals and are able to detect the toxicological effects of very low levels of toxic environmental pollutants. [0003] The application of zebrafish in toxicology has developed rapidly in recent years. In order to identify the toxicity of environmental pollutants, we need to analyze the correlation between zebrafish toxicological endpoints and pollu...

AI Technical Summary

Problems solved by technology

However, this method has certain limitations

First of all, the organism is an organic whole. From exposure to the exposure to the body’s response, various organs in the body need to coordinate with each other. When the exposure concentration exceeds the limit of the organism’s tolerance, the body’s damage will appear; secondly, in In daily life, human beings are often exposed to environmental pollutants at low doses for a long period of time. Exposure experiments with physical damage as the endpoint require high exposure concentrations and long exposure times. In this way, when the experimental results are extrapolated to the human body, There will be a lot of uncertainty and inaccuracy

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