Method for detecting development toxicity of environmental pollutants by whole-mount in-situ hybridization technique

A technology of environmental pollutants and in situ hybridization, which is applied in the direction of biochemical equipment and methods, and microbial measurement/inspection, can solve the problems of uncertainty, inaccuracy, long exposure time, body damage, etc., and shorten the detection cycle Effect

Inactive Publication Date: 2017-01-04
CHONGQING INST OF GREEN & INTELLIGENT TECH CHINESE ACADEMY OF SCI
View PDF2 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method has certain limitations
First of all, the organism is an organic whole. From exposure to the exposure to the body’s response, various organs in the body need to coordinate with each other. When the exposure concentration exceeds the limit of the organism’s tolerance, the body’s damage will appear; secondly, in In daily life,

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting development toxicity of environmental pollutants by whole-mount in-situ hybridization technique
  • Method for detecting development toxicity of environmental pollutants by whole-mount in-situ hybridization technique
  • Method for detecting development toxicity of environmental pollutants by whole-mount in-situ hybridization technique

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] In this example, according to the embryonic blastocyst predetermined fate map (fate map), the zebrafish blastocyst predetermined fate gene notochord mesoderm marker gene ntl is selected as the biomarker gene, and the environmental pollutants (using nano-silver as the Example) the impact on the changes in the transcription and expression of the biomarker gene ntl to evaluate the potential developmental toxicity of environmental pollutants (taking nano-silver as an example) on the early organs and tissues of zebrafish.

[0035] 1. Preparation of RNA probes for hybridization:

[0036] 1. Total RNA extraction: Take about 30 mg of fresh tissue samples of wild-type zebrafish, add 600 μl Trizol to fully grind, lyse at room temperature for 10 minutes, add chloroform, shake and mix well, let stand at room temperature for 10 minutes to extract, centrifuge at 12000 rpm for 15 minutes, absorb the supernatant and add isopropanol The precipitate was collected by centrifugation at 120...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the technical field of biology, and particularly relates to a method for detecting development toxicity of environmental pollutants by a whole-mount in-situ hybridization technique. The method comprises the following step: detecting the development toxicity of environmental pollutants to zebra fish embryos by a whole-mount in-situ hybridization technique. The method can be used for predicting potential toxic actions of the environmental pollutants on zebra fish organs and tissues, thereby further disclosing the action mode of the environmental pollutants, shortening the detection period, and achieving the goal of early warning in deed.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for detecting the developmental toxicity of environmental pollutants by using the overall in situ hybridization technique. Background technique [0002] Zebrafish is an important model organism, and its gene similarity with human genes is as high as about 87%. The experimental results obtained with zebrafish as experimental materials have great reference significance for the human body; therefore, zebrafish is very useful in toxicology research. Widely, especially zebrafish embryos are highly toxically sensitive to chemicals and are able to detect the toxicological effects of very low levels of toxic environmental pollutants. [0003] The application of zebrafish in toxicology has developed rapidly in recent years. In order to identify the toxicity of environmental pollutants, we need to analyze the correlation between zebrafish toxicological endpoints and pollu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/68
CPCC12Q1/6804C12Q1/6888C12Q2600/158C12Q2543/10C12Q2563/131
Inventor 裴得胜谢少林陈秋牛阿萍
Owner CHONGQING INST OF GREEN & INTELLIGENT TECH CHINESE ACADEMY OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap