Method for detecting carotenoid content of tea leaves by confocal micro-Raman

A confocal microscopy and carotene technology, applied in Raman scattering, material excitation analysis, etc., can solve the problems of unstable carotenoid properties, destructive properties, cumbersome detection steps, and inability to achieve online detection, etc. Economic benefits, shortened detection time, and simple structure

Active Publication Date: 2017-01-04
ZHEJIANG UNIV
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional detection methods are destructive, the detection steps are cumbersome, and online detection cannot be realized
At the same time, the properties of carotenoids in tea are not very

Method used

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  • Method for detecting carotenoid content of tea leaves by confocal micro-Raman
  • Method for detecting carotenoid content of tea leaves by confocal micro-Raman
  • Method for detecting carotenoid content of tea leaves by confocal micro-Raman

Examples

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Example Embodiment

[0062] Example 1

[0063] The sample stage structure provided by the present invention is as follows Figure 4 As shown, it includes: a slide glass 2, a cover glass 1 and a stage 3. Both the slide glass 2 and the cover glass 1 are made of quartz.

[0064] The surface of the slide glass 2 is used to place tea samples. The slide glass 2 is rectangular. Two long sides of the slide glass 2 are fixed with a slider 7 respectively. Each slider 7 is fixed with a spring pin and two sliders 7 The position of the spring pin on the corresponding. A silicon chip 5 is embedded on the glass slide 2.

[0065] The cover glass 1 is rectangular, the long side of the cover glass 1 is hinged with one of the sliders 7, and the cover glass 1 is turned over to compress the tea sample. The cover glass 1 is used to keep the surface of the tea leaf flat. When detecting different points on the tea sample, try to minimize the variation of the object distance to ensure that the Raman detection lens can be accu...

Example Embodiment

[0067] Example 2

[0068] Take 315 Longjing 43 leaves. For each Longjing 43 leaves, randomly cut the leaves on both sides of the veins to obtain two tea samples. The mass of each tea sample is about 0.1g. After the cutting is completed, the actual measurement and records Ww. One part is placed directly in the bag and labeled as Raman spectroscopy material A. The other part is cut into a centrifuge tube and labeled. Add 10mL of 95% alcohol solution and store it in a dark room for about 24h , As ultraviolet spectrophotometric material B. For the same tea leaf, Raman spectroscopy material A and ultraviolet spectrophotometric material B have the same label.

[0069] The Raman spectroscopy material A is fixed on the Raman stage described in Example 1, using a Renishaw confocal laser Raman microscope (Renishaw in Via-Reflex 532 / XYZ), excitation wavelength 532nm; laser intensity 50mW; Integration time 1s; Raman spectrum detection wavelength range is 579.175~3061.95cm -1 ; Resolution 0....

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Abstract

The invention discloses a method for detecting the carotenoid content of tea leaves by a confocal micro-Raman. The method includes the steps: 1) placing a tea leaf sample on a sample table, acquiring Raman spectral information of the tea leaf sample by a Raman spectrometer and performing full-wave band integral compensation; 2) building a linear model by the Raman spectral intensity of 17 characteristic wave numbers; 3) acquiring the corrected spectral intensity of the tea leaf sample to be detected at the 17 characteristic wave numbers, predicting the carotenoid content of the tea leaves by the linear model, and realizing nondestructive and rapid detection of the carotenoid content of the tea leaves. The carotenoid content of the tea leaves can be rapidly and effectively detected in a nondestructive, low-cost and environment-friendly manner without complicated pretreatment and chemical analysis for the tea leaf sample, operation steps are greatly simplified, detection time is shortened, information of carotenoid components in the tea leaves is rapidly acquired, and detection accuracy is improved.

Description

technical field [0001] The invention relates to the technical field of carotenoid content detection, in particular to a method for detecting carotenoid content in tea by confocal micro-Raman. Background technique [0002] Carotenoids are yellow-red pigments that widely exist in microorganisms, plants, animals and humans, and are essential components of living organisms. Carotenoids have two main functions in the photosynthesis of plants, one is to protect the photosynthetic system under the condition of excessive light irradiation, and the other is to absorb light energy as an antenna dye. [0003] Vegetation pigment content has a good correlation with its photosynthetic ability, developmental stage and nutritional status, and is usually an indicator of vegetation environmental stress, photosynthetic ability and vegetation developmental stage. Carotenoids not only endow various organisms with brilliant colors, but also serve as auxiliary pigments for photosynthesis, which n...

Claims

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Application Information

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IPC IPC(8): G01N21/65
CPCG01N21/65
Inventor 李晓丽许凯雯何勇周瑞清张裕莹魏玉震
Owner ZHEJIANG UNIV
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