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Application of a kind of polymer and the composition of stabilizing enzyme and chromogen

A technology of polymer and composition, applied in the field of medical testing, can solve the problems of deviation of test results, unobtainable raw materials, and high blank control, and achieve the effects of small deviation of test results, high accuracy and improved stability

Active Publication Date: 2019-05-10
SINOCARE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the dye added by the above method is sometimes close to the maximum absorption wavelength of the chromogenic agent, which makes the blank control higher
[0012] The main defects of the above-mentioned various protective agents for enzymes or chromogenic agents are high cost, unavailable raw materials, deviations in test results, and only applicable to enzyme-catalyzed color reactions in wet chemistry.

Method used

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  • Application of a kind of polymer and the composition of stabilizing enzyme and chromogen
  • Application of a kind of polymer and the composition of stabilizing enzyme and chromogen
  • Application of a kind of polymer and the composition of stabilizing enzyme and chromogen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Embodiment 1: polyethylene glycol methacrylate (R 1 :CH 3 C=CH 2 -, R 2 :-COO-,R 3 :-(CH 2 CH 2 O) n H, n=4-20)

[0059] Test items: total cholesterol;

[0060] Enzymes and color indicators required for detection items: cholesterol esterase, cholesterol oxidase, peroxidase, 4AA, MAOS;

[0061] Polymer: polyethylene glycol methacrylate;

[0062] Prepare different treatment groups according to Table 1 for project testing:

[0063] Table 1

[0064]

Option A

Option B

Plan C

Plan D

Option E

cholesterol esterase

200KU / L

200KU / L

200KU / L

200KU / L

200KU / L

cholesterol oxidase

200KU / L

200KU / L

200KU / L

200KU / L

200KU / L

Peroxidase

200KU / L

200KU / L

200KU / L

200KU / L

200KU / L

4AA

50mM

50mM

50mM

50mM

50mM

MAOS

50mM

50mM

50mM

50mM

50mM

polyethylene glycol methacrylate

--

0.1％

0.5％

1％

2％

[0065]...

Embodiment 2

[0075] Embodiment 2: polypropylene glycol dimethacrylate (R 1 :CH 3 C=CH 2 -, R 2 :-COO-,R 3 :-(CH(CH 3 )CH 2 O) n COC(CH 3 )=CH 2 , n=4-20); Castor oil polyoxyethylene ether (R 1 :CH 3 (CH 2 ) 5 CH(OH)CH 2 CH=CH(CH 2 ) 7 -, R 2 :-COO-,R 3 :-(CH 2 CH 2 O) n H, n=4-20)

[0076] Test items: total cholesterol;

[0077] Enzymes and color indicators required for detection items: cholesterol esterase, cholesterol oxidase, peroxidase, 4AA, MADB;

[0078] Polymers: polypropylene glycol dimethacrylate and castor oil ethoxylates;

[0079] Surfactant: Tx-100, Tween20;

[0080] Auxiliary stabilizer: sucrose, Gantrez AN-119;

[0081] Buffer system: PBS;

[0082] Prepare different treatment groups according to Table 3 for project testing:

[0083] table 3

[0084]

[0085] Use hydrochloric acid or sodium hydroxide to adjust the pH value to 6.4, and the test wavelength is 630nm;

[0086] Test process: with embodiment 1, the results are shown in Table 4.

[008...

Embodiment 3

[0090] Embodiment 3: polyethylene glycol dimethacrylate (R 1 :CH 2 =C(CH 3 )CH(CH 3 )-, R 2 :-COO-,R 3 :-(CH 2 CH 2 O) n COC(CH 3 )=CH 2 , n=4-20); Castor oil polyoxyethylene ether (R 1 :CH 3 (CH 2 ) 5 CH(OH)CH 2 CH=CH(CH 2 ) 7 -, R 2 :-COO-,R 3 :-(CH 2 CH 2 O) n H, n=4-20)

[0091] Test items: total cholesterol;

[0092] Enzymes and color indicators required for detection items: cholesterol esterase, cholesterol oxidase, peroxidase, 4AA, MADB;

[0093] Polymers: polyethylene glycol dimethacrylate and castor oil ethoxylates;

[0094] Surfactant: Tx-100;

[0095] Auxiliary stabilizer: sucrose, Gantrez AN-119;

[0096] Buffer system: PBS;

[0097] Prepare different treatment groups according to Table 5 for project testing:

[0098] table 5

[0099]

[0100]

[0101] Use hydrochloric acid or sodium hydroxide to adjust the pH value to 6.4, and the test wavelength is 630nm;

[0102] Test process: with embodiment 1, the results are shown in Table 6. ...

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Abstract

The invention relates to the field of medicine detection, and specifically discloses an application of a polymer and a composition for stabilizing an enzyme and a developer. The polymer has a general structure of R1-R2-R3, wherein R1 is selected from an alkene unsaturated group or an alkene unsaturated monomer; R2 is selected from -COO- or -O-; and R3 is selected from a polymer group of polyethylene glycol, poly-oxypropylene and polypropylene glycol. The invention provides an application of the polymer. The polymer interacts with the enzyme and the developer, thereby improving the stability of the enzyme and the developer. In multiple in-viro test items, the stability of the enzyme and the developer is kept in the extreme environment (such as high temperature). The test result is small in deviation and high in accuracy. The polymer together with other components forms a composition for stabilizing the enzyme and developer. The composition can be applied to an enzymic color development reaction, preparation of an in-viro detection product based on an enzymic color development reaction principle, and preparation of a stabilizer of the enzyme and the developer.

Description

technical field [0001] The invention relates to the field of medical detection, in particular to the application of a polymer and the composition of a stable enzyme and a color developing agent. Background technique [0002] As a method of determination (qualitative and quantitative) of target components, redox reaction is the most common type of color reaction. Enzyme-catalyzed color reaction is the most widely used one. The principle of its application in dry chemical detection is: immobilize the required reaction reagents and required enzymes on the membrane carrier, drop the sample to make the target component to be tested Generate oxidized substances, use oxidase to catalyze the decomposition of the oxide, and release new ecological oxygen. The new ecological oxygen reacts with the chromogenic agent to form a color-developing compound. By detecting the generated color-developing compound, the target component can be determined indirectly. [0003] As mentioned above, i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78
CPCG01N21/78G01N2021/775
Inventor 李凤叶吉翔彭希望李宗祥蔡晓华
Owner SINOCARE
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