Graphene sensor for rapidly detecting Gram negative bacteria lipopolysaccharide and preparation method

A Gram-negative, graphene-based technology, applied in the fields of biochemical detection technology and clinical medical diagnosis, can solve the problems of cumbersome operation steps, inconvenient promotion, and long time-consuming, and achieve simple process, short test time, and reduced false positive results Effect

Inactive Publication Date: 2017-01-04
天津喜诺生物医药有限公司
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  • Abstract
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  • Claims
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Problems solved by technology

However, the current endotoxin detection products based on LAL or C factor take a long time and the operation steps are cumbersome, which is not convenient for the promotion of clinical detection

Method used

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  • Graphene sensor for rapidly detecting Gram negative bacteria lipopolysaccharide and preparation method

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preparation example Construction

[0033] A kind of preparation method of the graphene sensor of quick detection Gram-negative bacterial lipopolysaccharide, it comprises steps as follows:

[0034] (1) preparing graphene, transferring the graphene material and uniformly and continuously covering the upper surface of the insulating substrate to form a graphene layer;

[0035] (2) Prepare the source electrode and the drain electrode at the two ends of the graphene layer respectively, prepare the back gate electrode on the lower bottom surface of the insulating substrate, and lead out the wires from the three electrodes respectively;

[0036] (3) Carry out surface treatment to graphene layer and modify antibody;

[0037] (4) A sample cell for adding samples is arranged in the middle of the graphene layer, and the outside of the sample cell is insulated and encapsulated.

[0038] In the specific implementation of the present invention, the concrete method of surface treatment of graphene layer in the described step...

Embodiment 1

[0042] A graphene sensor for the rapid determination of lipopolysaccharide in Gram-negative bacteria, as shown in the attached figure 1As shown, it consists of an insulating substrate, a back gate electrode on one side of the substrate, an antibody-modified graphene layer on the other side of the substrate, source and drain electrodes at both ends of the graphene layer, and a sample cell above the graphene . Graphene is a single-layer graphene prepared by chemical vapor deposition, which is uniformly and continuously covered on the PET insulating substrate by transfer; Ag source and Ag drain are respectively prepared at both ends of the graphene by screen printing. On the other side of the insulating substrate, Cu gate electrodes were prepared, and wires were drawn from the three electrodes; then the graphene side was first treated with 5 mmol / L PASE dimethyl sulfoxide solution at room temperature for 1 hour, and then ethanol and deionized Wash with water in sequence, then tr...

Embodiment 2

[0045] A graphene sensor for rapid determination of lipopolysaccharide of Gram-negative bacteria, consisting of an insulating substrate, a back gate electrode on one side of the substrate, an antibody-modified graphene layer on the other side of the substrate, and source electrodes arranged at both ends of the graphene layer Consists of a drain and a sample cell located above the graphene. Graphene is a single-layer graphene prepared by a redox method. The aqueous dispersion of the obtained graphene is repeatedly drip-coated on the PMMA insulating substrate to form a uniform continuous layer with a thickness of five layers of graphene; Prepare Au source and Au drain at both ends, prepare Ti gate electrode on the other side of the insulating substrate, and lead out wires from the three electrodes respectively; The solution was treated at room temperature for 1 hour, then washed sequentially with ethanol and deionized water, and then treated with 500 μmol / L factor C in PBS solut...

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Abstract

The invention discloses a graphene sensor for rapidly detecting Gram negative bacteria lipopolysaccharide and a preparation method thereof. The sensor consists of an insulating substrate, a back gate electrode at one side of the substrate, an antibody-decorated graphene layer at the other side of the substrate, and a source electrode and a drain electrode, which are disposed at two ends of the graphene layer, and a sample cell disposed above the graphene. Factors C decorated on the surface of the graphene layer are specifically combined with the Gram negative bacteria lipopolysaccharide to change the electrical properties of the graphene sensor, the content of the Gram negative bacteria lipopolysaccharide can be quantitatively determined by virtue of an external circuit so as to instruct the diagnosis of clinical diseases. The sensor can rapidly simply sensitively and specifically determine the Gram negative bacteria lipopolysaccharide. Meanwhile, by virtue of the preparation method, the stable and low-cost mass preparation of the graphene-based biological sensor can be realized, and high efficiency and accuracy in biological detection can be facilitated.

Description

technical field [0001] The invention belongs to the technical fields of biochemical detection technology and clinical medical diagnosis, in particular to a graphene sensor for rapidly detecting lipopolysaccharide of Gram-negative bacteria and a preparation method thereof. Background technique [0002] Bacterial endotoxin is a unique structure on the cell wall of Gram-negative bacteria, and its main chemical component is lipopolysaccharide. When a small amount (125ng / kg) of bacterial endotoxin enters the body, it can cause a series of pathological and physiological reactions such as fever, vasodilation, increased vascular permeability, increased neutrophils, complement activation, and decreased blood pressure in the body. Sometimes it can even lead to disseminated intravascular coagulation and multiple organ failure until shock death. At present, the detection of endotoxin levels in body fluids is a reliable indicator for the diagnosis of severe bacterial inflammation, sepsi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48
CPCG01N27/48
Inventor 唐城黄炎彬梁河立李世林
Owner 天津喜诺生物医药有限公司
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