Skin whitening and anti-skin aging butterfly orchid extractant
An anti-aging and skin whitening technology, applied in skin care preparations, cosmetics, cosmetic preparations, etc., can solve the problems of limited efficacy, unevenness, and low proportion of active ingredients
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Embodiment 1
[0019] The preparation of embodiment 1 Phalaenopsis extract
[0020] First, I purchased Phalaenopsis V3 from the market. Its scientific name is Phalaenopsis Sogo Yukidian ‘V3’ in English. The petals are picked, and the pedicel is cut to an appropriate length at the same time, washed several times with sterile water, and then placed in an oven set at 45-60° C. for drying before use.
[0021] In this example, multiple extraction methods will be carried out at the same time to compare and find out the extraction conditions with better effect extracts.
[0022] (1) Water extraction
[0023] Take the dried Phalaenopsis petals or pedicels as the extract, add pure water at a weight ratio of 1:10, stir and mix to form a mixture, and heat the mixture to 100°C for 90 minutes for extraction. Afterwards, the extracted mixture was filtered through filter paper with a pore size of 6 μm to obtain a water-soluble Phalaenopsis extract solution.
[0024] (2) Alcohol extraction after water ex...
Embodiment 2
[0031] Example 2 In vitro tyrosinase activity test
[0032] Add the aforementioned powdered Phalaenopsis extract to ddH 2 O, configured as a sample solution to be tested with a concentration of 10% (w / v) for standby use. Take 200 μl of the prepared sample solution to be tested and 800 μl of 0.1M phosphate buffer solution (pH 6.8), and inject them into the microtube. In the control group, the sample solution to be tested was replaced with an equal volume of 0.1M phosphate buffer solution. Afterwards, add 100 μl of 70 unit / ml mushroom tyrosinase to the microtubes of all the sample solutions to be tested and the control group, and finally add 900 μl of 25 mM L-DOPA, mix the solution well, and react at 37°C for 30 minutes . After the reaction was completed, the absorbance value at a wavelength of 405 nm was measured with a spectrophotometer, and the results are shown in Table 1.
[0033]If there is a component that inhibits tyrosinase in the sample to be tested, the original r...
Embodiment 3
[0041] Embodiment 3 antioxidant power test
[0042] In this example, the test on the antioxidant capacity was evaluated by the ability of Phalaenopsis extract to scavenge α,α-diphenyl–β–picrylhydrazyl (α,α-diphenyl–β–picrylhydrazyl, DPPH) free radicals Antioxidant capacity.
[0043] Take 0.2ml of the sample solution to be tested with different concentrations, add 1.8ml of freshly prepared 0.1mM DPPH methanol solution, shake and mix evenly, and react at room temperature for 30 minutes in a light-proof environment, and then use a spectrophotometer to detect the concentration at 517nm wavelength. absorbance value. In the control group, an equal volume of 0.1 mM DPPH ethanol solution was used instead of the sample solution to be tested, and then the same reaction test as above was carried out, and the results are shown in Table 2.
[0044] Since substances with antioxidant capacity will provide hydrogen to DPPH free radicals, the oxidation chain reaction will be inhibited. There...
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