Preparation method and application of PCT electrochemical immunosensor based on AuNPs/Cu-MOF marking

An immunosensor and electrochemical technology, applied in the field of electrochemical immunosensor construction, can solve the problems of complex pretreatment, expensive, cumbersome operation, etc., and achieve the effects of sensitive electrochemical signals, promoting electron transfer, and increasing the load capacity

Inactive Publication Date: 2017-01-11
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These methods have high sensitivity and selectivity, but the detection process requires expensive special instruments,

Method used

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  • Preparation method and application of PCT electrochemical immunosensor based on AuNPs/Cu-MOF marking
  • Preparation method and application of PCT electrochemical immunosensor based on AuNPs/Cu-MOF marking

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 A method for preparing a PCT electrochemical immunosensor based on AuNPs / Cu-MOF labeling

[0036] (1) Use 1.0 μm, 0.3 μm, 0.05 μm Al 2 o 3 The glassy carbon electrode (GCE) with a diameter of 4 mm was polished successively with polishing powder, ultrasonically cleaned in ethanol and ultrapure water for 3 min, and dried with nitrogen gas, and 6 μL of AuNFs dispersion was applied onto the surface of the electrode, and dried at room temperature to form a The membrane was washed with PBS (pH 7.4) buffer solution to remove unbound AuNFs to obtain AuNFs / GCE;

[0037] (2) Take 10 μL of 6 μg / mL procalcitonin primary antibody (Ab 1 ) standard solution onto the surface of the electrode, incubated overnight at 4 °C, washed with PBS (pH 7.4) buffer solution to obtain Ab 1 / AuNFs / GCE;

[0038] (3) Take 10 μL bovine serum albumin (BSA) solution with a mass fraction of 1% drop-coated on the surface of the electrode, incubate at 37°C for 1 h, block the non-specific bindin...

Embodiment 2

[0041] Example 2 A method for preparing a PCT electrochemical immunosensor based on AuNPs / Cu-MOF labeling

[0042] (1) Use 1.0 μm, 0.3 μm, 0.05 μm Al 2 o 3 The glassy carbon electrode (GCE) with a diameter of 4 mm was polished successively with polishing powder, ultrasonically cleaned in ethanol and ultrapure water for 3 min, and dried with nitrogen gas, and 8 μL of AuNFs dispersion was applied onto the surface of the electrode, and dried at room temperature. The membrane was washed with PBS (pH 7.4) buffer solution to remove unbound AuNFs to obtain AuNFs / GCE;

[0043] (2) Take 10 μL of 8 μg / mL procalcitonin primary antibody (Ab 1 ) standard solution onto the surface of the electrode, incubated overnight at 4 °C, washed with PBS (pH 7.4) buffer solution to obtain Ab 1 / AuNFs / GCE;

[0044] (3) Take 10 μL of bovine serum albumin (BSA) solution with a mass fraction of 2% and drop-coat it on the surface of the electrode, incubate at 37°C for 1 h, block the non-specific binding...

Embodiment 3

[0047] Example 3 A method for preparing a PCT electrochemical immunosensor based on AuNPs / Cu-MOF labeling

[0048] (1) Use 1.0 μm, 0.3 μm, 0.05 μm Al 2 o 3 The glassy carbon electrode (GCE) with a diameter of 4 mm was polished successively with polishing powder, ultrasonically cleaned in ethanol and ultrapure water for 3 min, and dried with nitrogen gas, and 10 μL of AuNFs dispersion was applied onto the surface of the electrode, and dried at room temperature. The membrane was washed with PBS (pH 7.4) buffer solution to remove unbound AuNFs to obtain AuNFs / GCE;

[0049] (2) Take 10 μL of 10 μg / mL procalcitonin primary antibody (Ab 1 ) standard solution onto the surface of the electrode, incubated overnight at 4 °C, washed with PBS (pH 7.4) buffer solution to obtain Ab 1 / AuNFs / GCE;

[0050] (3) Take 10 μL of bovine serum albumin (BSA) solution with a mass fraction of 3% and drop-coat it on the surface of the electrode, incubate at 37°C for 1 h, block the non-specific bindi...

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Abstract

The invention relates to the field of preparation of an electrochemical immunosensor for rapid and sensitive detection of procalcitonin antigens, and in particular relates to a preparation method and application of a PCT electrochemical immunosensor based on AuNPs/Cu-MOF marking. AuNPs has a large specific surface area, has good conductibility and good biocompatibility, can capture many primary antibodies as a base material, and simultaneously can promote electron transfer; AuNPs/Cu-MOF marks secondary antibodies and can provide sensitive electrochemical signals; and according to the relationship between the intensity change of the electrochemical signals of AuNPs/Cu-MOF and the concentration of the antigens, rapid and sensitive detection of the procalcitonin antigens can be realized.

Description

technical field [0001] The invention relates to the technical field of electrochemical immunosensing, in particular to a preparation method and application of an AuNPs / Cu-MOF-labeled PCT electrochemical immunosensor. It specifically relates to a method for constructing an electrochemical immunosensor in which AuNFs are used as antibody capture substrates and AuNPs / Cu-MOF-labeled secondary antibodies are used as redox probes. Background technique [0002] PCT belongs to a kind of calcitonin propeptide substance, which has no hormonal activity. The increase in serum level is caused by severe infection and destruction of bacteria, fungi, and parasites, as well as sepsis and multiple organ failure. PCT in In autoimmunity, allergies and viral infections, the content will not increase. Therefore, PCT can diagnose systemic infections caused by bacteria and fungi, and PCT will be regarded as a routine indicator for auxiliary and differential diagnosis of systemic bacterial infectio...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N33/53
CPCG01N27/3277G01N33/53
Inventor 花小霞周长利薛华瑞
Owner UNIV OF JINAN
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