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Tissue culture method of acer rubrum

A safflower maple, tissue culture technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., to achieve the effects of low pollution rate, scientific steps, and high induction rate

Inactive Publication Date: 2017-01-18
盛世绿源科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no research on the tissue culture of Acer safflower "October Glory" in our country. Therefore, strengthening the research on its asexual reproduction, establishing an asexual reproduction technology system, and improving the production capacity of Acer safflower seedlings in my country can be widely used in urban gardens. Provide technical support in

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] This embodiment discloses a method for tissue culture of Acer safflower, comprising the following steps:

[0032] Step 1, cut the dormant buds of the Acer japonica plant individual and carry out pretreatment, then inoculate on the induction medium containing 0.05mg / L cytokinin, 0.05mg / L auxin, 30g / L sucrose and 6g / L agar, At a temperature of 25°C, a humidity of 60%, and a light intensity of 50 μmol m -2 ·s -1 , 20 hours of light per day, 20 days to obtain sterile sprouts;

[0033] Step 2: Cut the obtained sterile sprouts into 1.5-3.0cm stem segments with terminal buds, inoculate them with 0.05mg / L cytokinin, 0.05mg / L auxin, 30g / L sucrose and 6g / L On the induction medium of agar, at a temperature of 25°C, a humidity of 60%, and a light intensity of 800 μmol·m -2 ·s -1 1. Cultivate for 2 months under the conditions of 18 hours of light per day and replacement of fresh medium every 25 days to obtain clustered sprouts;

[0034] Step 3: Select the strong clustered sprouts...

Embodiment 2

[0040] This embodiment discloses a method for tissue culture of Acer safflower, comprising the following steps:

[0041] Step 1, cut the dormant buds of the Acer japonica plant individual and carry out pretreatment, then inoculate on the induction medium containing 0.1mg / L cytokinin, 0.08mg / L auxin, 20g / L sucrose and 7g / L agar, At a temperature of 20°C, a humidity of 60%, and a light intensity of 20 μmol m -2 ·s -1 , 12 hours of light per day, 30 days to obtain sterile sprouts;

[0042] Step 2: Cut the obtained sterile sprouts into 1.5-3.0cm stem segments with terminal buds, inoculate them with 0.03mg / L cytokinin, 0.05mg / L auxin, 20g / L sucrose and 7g / L On the induction medium of agar, at a temperature of 30°C, a humidity of 80%, and a light intensity of 20 μmol·m -2 ·s -1 1. Cultivate for 1 to 3 months under the conditions of 12 hours of light per day and replacement of fresh medium every 30 days to obtain clustered sprouts;

[0043] Step 3: Select the strong clustered sp...

Embodiment 3

[0049] This embodiment discloses a method for tissue culture of Acer safflower, comprising the following steps:

[0050] Step 1, cut the dormant buds of the Acer japonica plant individual and carry out pretreatment, then inoculate on the induction medium containing 0.02mg / L cytokinin, 0.05mg / L auxin, 20g / L sucrose and 5g / L agar, At a temperature of 20°C, a humidity of 70%, and a light intensity of 20 μmol m -2 ·s -1 , 24h and 30 days of light every day to obtain sterile sprouts;

[0051] Step 2: Cut the obtained sterile sprouts into 1.5-3.0cm stem segments with terminal buds, inoculate them with 0.06mg / L cytokinin, 0.08mg / L auxin, 35g / L sucrose and 5g / L On the induction medium of agar, at a temperature of 25°C, a humidity of 50%, and a light intensity of 80 μmol·m -2 ·s -1 1. Cultivate for 2 months under the conditions of 15 hours of light per day and replacement of fresh medium every 30 days to obtain clustered sprouts;

[0052] Step 3: Select the strong clustered sprout...

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Abstract

The invention provides a tissue culture method of acer rubrum, comprising the following steps: cutting dormant buds from acer rubrum plants and pretreating, inoculating the pretreated dormant buds to an inducing medium to obtain aseptic shoots; inoculating the obtained aseptic shoots to an inducing medium to obtain crowd shoots; selecting robust crowd shoots and inoculating the robust crowd shoots to a strong seedling medium to obtain robust seedlings; selecting robust seedlings to a rooting medium to obtain regeneration plants; hardening seedling on the regeneration plants in an open hardening seedling room for 3d, washing the medium attached to the root system, transplanting the plants into a culture medium and watering, covering the mouth of a container with a plastic film until new leaves are fully expanded, and removing the covered plastic film so as to obtain the acer rubrum nursery stock. The tissue culture method of acer rubrum has scientific and reasonable steps, is simple and efficient, and has advantages of low cost, high inductivity, high multiplication coefficient and high survival rate.

Description

technical field [0001] The invention relates to tissue culture technology, in particular to a tissue culture method of Acer safflower. Background technique [0002] American red maple (scientific name: Acer rubrum L.): Also known as red maple, it is native to the east coast of the United States, mainly from the northern part of the United States and most of Canada. It is a large deciduous tree. It grows fast, with an adult height of 12-18 meters and a crown width of 12 meters. It can adapt to a variety of soil types. Flowering in spring, red flowers. Because of its eye-catching colors in autumn and neat tree crown, it is widely used in parks, residential areas, and streets for planting. It can be used for landscaping and street trees. It is deeply loved by people. It is an ideal rare for landscaping and greening urban gardens introduced in recent years. One of the tree species. It is also the only color-leaved tree species that can be used as a street tree. This species...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 马丽源孔婷
Owner 盛世绿源科技有限公司
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