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Cirrhinus molitorella bone collagen extracting method

A technology of bone collagen and extraction method, which is applied in the field of extraction of dace bone collagen, and can solve problems such as calcium loss

Inactive Publication Date: 2017-01-18
GUANGDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the existing methods for preparing collagen from leftovers such as fish bones, when extracting bone collagen, the calcium is often removed first and then the collagen is hydrolyzed to prepare purer collagen, but this will cause calcium massive loss

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] (1) Pretreatment of waste fish bones:

[0042] Impurities such as swim bladder are manually removed, the dace bone raw material is put into a container, water is added to submerge the fish bone, and protease solution is added to the container. Put the container into an electric heating water bath, set the temperature to 60°C, and the water bath time to 30min. After the water bath, heat in boiling water for 10 minutes to inactivate the enzyme. Clean the fish bones to remove residual fish meat. The cleaned fish bones are dried;

[0043] (2) Crushing treatment:

[0044] Grinding the dried fish bones with a high-speed pulverizer to obtain coarse fish bone powder;

[0045] (3) Degreasing treatment of fishbone meal:

[0046] Use n-butanol to soak the coarse fishbone powder for 6 hours, the ratio of solid to liquid is 1:30, and then rinse with water.

[0047] (4) Miscellaneous protein removal:

[0048] At room temperature, first use 0.05mol / L NaOH solution to soak the c...

Embodiment 2

[0052] (1) Pretreatment of waste fish bones:

[0053] Impurities such as swim bladder are manually removed, the dace bone raw material is put into a container, water is added to submerge the fish bone, and protease solution is added to the container. Put the container into an electric heating water bath, set the temperature to 60°C, and the water bath time to 30min. After the water bath, heat in boiling water for 10 minutes to inactivate the enzyme. Clean the fish bones to remove residual fish meat. The cleaned fish bones are dried;

[0054] (2) Crushing treatment:

[0055] Grinding the dried fish bones with a high-speed pulverizer to obtain coarse fish bone powder;

[0056] (3) Degreasing treatment of fishbone meal:

[0057] Use isopropanol to soak the coarse fishbone powder for 6 hours, the ratio of solid to liquid is 1:30, and then rinse with water.

[0058] (4) Miscellaneous protein removal:

[0059] At room temperature, first use 0.05mol / L NaOH solution to soak the c...

Embodiment 3

[0063] (1) Pretreatment of waste fish bones:

[0064] Impurities such as swim bladder are manually removed, the dace bone raw material is put into a container, water is added to submerge the fish bone, and protease solution is added to the container. Put the container into an electric heating water bath, set the temperature to 60°C, and the water bath time to 30min. After the water bath, heat in boiling water for 10 minutes to inactivate the enzyme. Clean the fish bones to remove residual fish meat. The cleaned fish bones are dried;

[0065] (2) Crushing treatment:

[0066] Grinding the dried fish bones with a high-speed pulverizer to obtain coarse fish bone powder;

[0067] (3) Degreasing treatment of fishbone meal:

[0068] Use acetone to soak the coarse fish bone powder for 12 hours, the ratio of solid to liquid is 1:30, and then rinse with water.

[0069] (4) Miscellaneous protein removal:

[0070] at room temperature,

[0071] Use 0.05mol / L NaOH solution to treat ...

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Abstract

The invention relates to the technical field of food industry, in particular to a cirrhinus molitorella bone collagen extracting method. In the preparation process of the extracting method, nosubstance such as acetic acid or EDTA causing calcium ion loss is not adopted, thus, the collagen content is higher in the cirrhinus molitorella bone collagen obtained by adopting the method, and the calcium ions in fish bones are also retained. It is detected that the collagen content in the cirrhinus molitorella bone collagen obtained by adopting the method is 13.9%-21.1%.

Description

technical field [0001] The invention relates to the technical field of food industry, in particular to a method for extracting dace bone collagen. Background technique [0002] Collagen is a structural protein of the extracellular matrix. It is a white, opaque, unbranched fibrous protein. It has attracted extensive attention in biomedical materials, food, and beauty because of its good biological properties. my country is a large country of aquatic products. With the expansion of aquaculture scale and the development of processing industry, many aquatic products, including their processed waste, are rich in collagen and can be a good source of collagen. [0003] Dace is one of the important economic fishes in South China. The leftovers such as fish bones and fish tails produced in the processing process account for about 40% to 55% of the raw fish quality. If it is not treated effectively, it will not only cause environmental pollution, but also waste a lot of precious reso...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K14/78C07K1/14
Inventor 丁利君毕艳梅梁任成刘丹
Owner GUANGDONG UNIV OF TECH
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