Preparation method of superior microorganism solid inoculants used for river water pollution management

A solid inoculum and microorganism technology, applied in the method of using microorganisms, water/sludge/sewage treatment, polluted waterway/lake/pond/river treatment, etc., can solve the problem of unfavorable internal microorganisms to obtain nutrients and dissolved oxygen. , affecting the effect and efficiency of water pollution control, the lack of toughness of chitosan, etc., to achieve the effect of facilitating the transfer and exchange of substances, improving the concentration and stability of microorganisms, and reducing the breakage rate

Inactive Publication Date: 2017-01-25
GUANGDONG ZHONGWEI ENVIRONMENTAL PROTECTION BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Common carriers include polyacrylamide, chitosan, etc., but monomeric acrylamide is cytotoxic and needs to be heated during the preparation process, which will cause a large number of microorganisms to be inactivated during the immobilization process, thus seriously affecting their performance in immobilization. In the application of microbial cells or immobilized enzymes, chitosan lacks toughness and is brittle after swelling in aqueous solution, which is not convenient for practical application, and the carrier material is closed in structure and does not have a pore structure, which is not conducive to the internal microorganisms to obtain nutrients and Dissolved oxygen, and emissions of microbial metabolites
Using these two materials as carriers to prepare solid bacterial agents will affect the effect and efficiency of subsequent water pollution control

Method used

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Embodiment Construction

[0020] The present invention will be further described below in conjunction with embodiment.

[0021] The method for preparing the solid bacterial agent of advantageous microorganisms for river water pollution control comprises the following steps:

[0022] (1) Domestication of microorganisms: collect the river water samples and sediments to be treated, mix them evenly according to the ratio of sediment (g): water sample (ml) = 1:2, and shake them in the Erlenmeyer flask for 10 minutes to obtain the domestication stock solution , put the domestication stock solution aside for later use; divide 100ml enrichment medium into six 250ml Erlenmeyer flasks; add 10ml of the supernatant of the acclimatization stock solution to the first Erlenmeyer flask, and carry out aerated culture at 30°C. For 5 days, use a sterile pipette to draw 10ml of the solution in the Erlenmeyer flask to another Erlenmeyer flask for cultivation, and after 5 consecutive transfers, obtain a microbial mixed cult...

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PUM

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Abstract

The invention relates to the field of river surge treatment, in particular to a method for preparing a dominant microbial solid bacterial agent for river water pollution control, which includes acclimating microorganisms, preparing a composite bacterial liquid, selecting a carrier and preparing a solid bacterial agent. On the one hand, before the preparation of the solid bacterial agent, the microorganisms were domesticated, and the obtained microbial mixture had an absolute advantage in enriching and cultivating the target bacteria, and the concentration was high. The microorganisms in the solid microbial agent could well colonize in the water body. It is conducive to improving the quality and efficiency of subsequent water pollution treatment; secondly, the solid bacterial agent is prepared by adsorption or embedding, the environmental requirements are not harsh, the reaction conditions are mild, and the operation is simple. The microorganisms in the composite bacterial liquid can be combined with the carrier The combination is tight, and the concentration of microorganisms in the prepared solid bacterial agent is high; in the third aspect, the carrier has a particle size of 1-3mm and a density of 1.1-1.5g / cm 3 The special ceramsite or molecular sieve carrier for porous organisms is non-toxic and has good biocompatibility, which is conducive to material transmission and exchange.

Description

technical field [0001] The invention relates to the field of river water pollution control, in particular to a method for preparing a dominant microbial solid bacterial agent for river water pollution control. Background technique [0002] With the development of social economy and the improvement of people's living standards, the dense population brings domestic sewage, and the spread of small factories brings industrial wastewater. These untreated domestic sewage, untreated or untreated industrial production wastewater are also discharged with When the organic pollutants exceed the limit of natural purification that the river water body can hold, the dissolved oxygen in the water will be consumed rapidly and become anoxic, and the river water body will become eutrophic. The water body turns black and smelly. Due to the lack of oxygen, the survival of the original ecological organisms is directly threatened, until death, corpse decay, further pollution of the water body and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N1/36C02F3/34
CPCC12N11/14C02F3/34C02F2103/007C12N1/36
Inventor 张保安黄来云邓志华张镇球
Owner GUANGDONG ZHONGWEI ENVIRONMENTAL PROTECTION BIOTECH CO LTD
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