Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for the advanced treatment of sewage by cultivating microalgae in series

A technology for advanced treatment and sewage, applied in the direction of microorganism-based methods, methods using microorganisms, chemical instruments and methods, etc., can solve the problems of low-transparency microalgae growth, difficulty in achieving ideal results, etc.

Active Publication Date: 2020-01-31
FUJIAN INST OF MICROBIOLOGY
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, factors such as high concentration of ammonia nitrogen and low transparency in the anaerobic effluent of pig raising sewage are not conducive to the growth of microalgae
Using a single microalgae to treat pig sewage is often difficult to achieve the desired effect

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: the cultivation of microalgae

[0017] Pick a small amount of Chlorella and Scenedesmus species from the vacuum freeze-drying tubes, add them to a conical flask containing 200mL of growth medium 1, and cultivate under full light, illuminance 4000-5000lx, continuous aeration, 25±1°C . It can be observed that the color of the culture medium turns green gradually. After 6-7 days, it will reach the logarithmic growth phase. In addition, pick a small amount of Spirulina algae species from the vacuum freeze-drying tube, add them to the Erlenmeyer flask containing 200mL of growth medium 2, and cultivate them under full light with an illumination of 4000-5000lx and continuous aeration at 30±1°C. It can be observed that the color of the culture medium turns green gradually. After 5 to 7 days, it will reach the logarithmic phase.

[0018] The preparation method of growth medium 1 is: glucose 3.0g, yeast powder 0.2g, peptone 1.5g, sodium acetate 1.6g, sodium chlo...

Embodiment 2

[0020] Embodiment 2: the domestication test of microalgae in the present invention

[0021] Chlorella, Spirulina and Scenedesmus were respectively domesticated and cultured for the anaerobic effluent of pig breeding sewage: take 40 mL of the microalgae liquid in the logarithmic growth phase of Example 1, centrifuge at 4500r / min for 10min, and discard the supernatant to obtain For microalgae, inoculate the microalgae into 200mL acclimation medium 1 (according to the total fraction of 100%, including pig sewage anaerobic effluent 10%+H 2 O 80%+growth medium 10%), illuminance 4000~5000lx, full light, 120rpm, in which Chlorella and Scenedesmus were cultured at 25±1°C, and Spirulina was cultivated at 30±1°C. It can be observed that the color of the culture solution gradually Turn green, wait for it to grow to the logarithmic growth phase, take 40mL of microalgae liquid, centrifuge at 4500r / min for 10min, discard the supernatant to obtain microalgae, inoculate the microalgae into 20...

Embodiment 3

[0023] Embodiment 3: Purification test of the anaerobic effluent of pig raising sewage by serial culture of microalgae in the present invention

[0024] The indicators of the anaerobic effluent of pig raising sewage are as follows: COD is 1355.19mg / L, total nitrogen (TN) is 561.23mg / L, and total phosphorus (TP) is 65.07mg / L. The algae fluids of Chlorella, Spirulina and Scenedesmus that have been acclimated to the anaerobic effluent of high-concentration pig-raising sewage were respectively taken, and cultivated to the logarithmic growth phase according to the method of Example 1 as the test algae species. Take 400mL of chlorella algae liquid, centrifuge at 4500r / min for 10min, discard the supernatant to obtain chlorella algae, and put the chlorella algae into 2L of treatment solution 1 (according to the total fraction of 100%, including pig sewage Anaerobic effluent 60%+H 2 O 40%, the initial COD of treatment liquid 1 is 813.11mg / L, and total nitrogen (TN) is 336.74mg / L, and ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for deep sewage treatment by serial microalgae culture. The method includes the steps: 1) microalgae domestication; 2) deep sewage treatment by serial microalgae culture. After anaerobic effluent is treated by the method, degradation rates of COD (chemical oxygen demand), TN (total nitrogen) and TP (total phosphorus) in the anaerobic effluent with the volume fraction of 60% can reach 96%, 94% and 93% respectively, and the degradation rates of the COD, the TN and the TP in the anaerobic effluent with the volume fraction of 40% can reach 95%, 97% and 94% respectively. Experimental results indicate that the treatment effect of serially cultured anaerobic effluent chlorella, spiral seaweeds and scenedesmus for deep treatment of the anaerobic effluent is remarkably better than that of individual use of the anaerobic effluent chlorella, the spiral seaweeds or the scenedesmus for deep treatment of the anaerobic effluent. By the aid of growth characteristic difference and different sewage treatment capacity of different types of microalgae, respective advantages of the microalgae are taken by serial microalgae culture, sewage is deep purified, and novel ideas and methods are provided for sewage treatment.

Description

Technical field: [0001] The invention belongs to the technical field of sewage treatment, and in particular relates to a method for cultivating microalgae in series for advanced treatment of sewage. Background technique: [0002] In recent years, with the development of the national economy and the improvement of the living standards of the people, the pig farming industry is developing in the direction of scale and intensification. Large-scale farming can greatly shorten the growth cycle and reduce the cost of farming, thereby increasing economic benefits. However, large-scale pig farms lead to concentrated discharge of feces and urine sewage. The amount of sewage is large, and it carries a lot of feces. The concentration of pollutants is high and the treatment is difficult, which has led to a series of environmental pollution problems. At present, the sewage treatment modes of the pig industry can be classified into two types: one is the return mode aimed at resource util...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C02F3/32C12N1/36C12R1/89
CPCC02F3/322C12N1/12C12N1/36
Inventor 聂毅磊陈宏贾纬罗立津
Owner FUJIAN INST OF MICROBIOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com