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A method of utilizing calcium chloride to treat Pichia pastoris to absorb rhodium ions in waste water

A Pichia pastoris and wastewater technology, applied in chemical instruments and methods, biological water/sewage treatment, water/sludge/sewage treatment, etc. problem, to achieve important industrial application value, easy operation, and obvious effect.

Active Publication Date: 2019-07-23
江苏骏驰环保科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Pyrometallurgy has the characteristics of simple process and high recovery rate. The disadvantages are high energy consumption, easy to produce secondary pollution, and the purity of rare and precious metals recovered by general pyrometallurgical processes is not high.
However, other methods for recovering rare and precious metals from liquid secondary resources such as wastewater and waste liquid, such as chemical precipitation, electrolysis, ion exchange, membrane technology separation and resin adsorption, etc., can achieve certain effects, but in the recovery For rare and precious metals, the operation is cumbersome, the investment and operation costs are high, and generally only the rare and precious metals in high-concentration metal waste liquid can be recovered, and a large amount of secondary waste is generated during the recycling process. Therefore, a low-cost and environmentally friendly rare and precious metal secondary Secondary resource recycling method

Method used

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  • A method of utilizing calcium chloride to treat Pichia pastoris to absorb rhodium ions in waste water
  • A method of utilizing calcium chloride to treat Pichia pastoris to absorb rhodium ions in waste water
  • A method of utilizing calcium chloride to treat Pichia pastoris to absorb rhodium ions in waste water

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Pick a single colony from the YPD plate and inoculate it into a 500mL Erlenmeyer flask containing 50mL seed medium, at 30°C, 220r min -1 The 24h of cultivation was used as the seed solution for fermenter cultivation. The seed solution is inoculated into a 5L fermenter containing 1.2L fermentation medium with an inoculation amount of 10% (volume ratio) and cultivated for 16-36 hours. Centrifuge at 7000rpm for 3-10min, collect the bacteria, and wash the bacteria 2-3 times with deionized water.

Embodiment 2

[0036] With 0.2mol / L CaCl 2 Suspend and mix 1 g (dry weight) of Pichia pastoris, stir slowly in a magnetic stirrer for 6-24 hours, centrifuge at 3000-5000 rpm, wash with deionized water for 3-4 times, and collect bacteria by centrifugation. Using the same volume of deionized water as a control, the cells were collected by stirring, washing and centrifuging in the same manner as above.

[0037] Prepare 100mL of 50mg / L rhodium ion solution in the Erlenmeyer flask, add the bacteria collected by the above method into the Erlenmeyer flask respectively, plug the mouth of the bottle with a cotton plug, treat at 30°C, 200rpm for 60min, calculate the adsorption rate, The result is as figure 1 shown, without CaCl 2 The rhodium ion adsorption rate of the treated Pichia pastoris was 20.3%, after CaCl 2 The rhodium ion adsorption rate of the treated Pichia pastoris was 78.2%.

Embodiment 3

[0039] With 0.2mol / L CaCl 2 Suspend and mix 1 g (dry weight) of Pichia pastoris, stir slowly in a magnetic stirrer for 6-24 hours, centrifuge at 3000-5000 rpm, wash with deionized water for 3-4 times, and collect bacteria by centrifugation.

[0040] Prepare 100mL of 50mg / L rhodium ion solution in an Erlenmeyer flask, use NaOH and HCl to adjust the pH=1 to 11, add the bacteria treated by the above method into the Erlenmeyer flask, treat at 30°C, 200rpm for 120min, calculate Adsorption rate. The result is as figure 2 As shown, when the pH is 7-11, the adsorption rate exceeds 84.9%, and when the pH is 9-11, the adsorption rate is 92.5-97.1%.

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Abstract

The invention discloses a method for adsorbing rhodium ions in wastewater by processing pichia pastoris through calcium chloride and belongs to the field of sewage treatment. CaCl2 treatment is conducted on pichia pastoris thalli, and the capacity of adsorbing rhodium ions in wastewater is improved. CaCl2 with the concentration of 0.1-0.3 mol / L is used for treating the pichia pastoris thalli, the thalli are added into sewage containing rhodium ions, the concentration of the thalli is adjusted to be 6-16 g / L, the pH is adjusted to be 9-11, treatment is conducted for 120-240 min at 30-45 DEG C at the speed of 200-220 rpm, 97.9% of the rhodium ions in sewage can be adsorbed, and the method is easy to operate and low in cost and has important industrial application value.

Description

technical field [0001] The invention relates to a method for treating Pichia pastoris to absorb rhodium ions in waste water by using calcium chloride, belonging to the field of sewage treatment. Background technique [0002] In order to prevent environmental pollution, industrial wastewater needs to be treated and discharged only after reaching the standard. There are many treatment methods for sewage containing heavy metals, such as chemical precipitation, ion exchange resin, activated carbon adsorption, electrolysis and membrane separation, but these methods are not ideal due to cumbersome operation and secondary pollution. [0003] On the other hand, in addition to the consideration of environmental safety in the treatment of sewage containing rare and precious metals, resource recovery is also an aspect that cannot be ignored. Traditional secondary resource recovery of rare and precious metals mainly adopts physical and chemical methods. Among them, pyrometallurgy tech...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/34C02F101/20
CPCC02F3/347C02F2101/20
Inventor 高敏杰洪瀚詹晓北李志涛沈宇峰郑志永
Owner 江苏骏驰环保科技有限公司