Method for extracting pentose through plant hemicellulose hydrolysis
A technology of hemicellulose and plants, which is applied in the field of high-efficiency hydrolysis of plant hemicellulose to obtain pentose sugar solution, which can solve the problems of unsatisfactory hydrolysis effect, consumption of chemicals and raw materials, waste of resources, etc., and is beneficial to resource utilization and hydrolysis Good effect, highly selective effect
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Embodiment 1
[0017] Weigh bamboo chips with an absolute dry weight of 2.4 g and a mesh number of 20 to 80 in a 250 mL ground conical flask, and add 0.3 mol / L according to the liquid-solid ratio of 60:1 (volume-to-mass ratio, in mL / g). The maleic acid solution was placed in a constant-temperature heating magnetic stirrer at 140 °C, and the magnetic stirring and condensing device was turned on, and the reaction was carried out for 8 h. After the hydrolysis reaction was completed, the conical flask was cooled to room temperature, filtered with a Buchner funnel, and the hydrolysis residue was washed with deionized water until neutral, and the obtained filtrate was the bamboo shavings hemicellulose hydrolyzate.
[0018] The content of pentose polysaccharide in the bamboo chips is 20.13% according to the national standard GB / T2677.9-1994, and the content of pentose sugars in the hemicellulose hydrolyzed solution is tested by the phloroglucinol method to be 0.413 g, and the pentose sugar content i...
Embodiment 2
[0024] Weigh bamboo chips with an absolute dry weight of 2.4 g and a mesh number between 20 and 80 into a 250 mL ground conical flask, and add 0.5 mol / L at a liquid-solid ratio of 50:1 (volume-to-mass ratio, in mL / g). The maleic acid solution was placed in a constant temperature heating magnetic stirrer at 150 °C, and the magnetic stirring and condensation device was turned on, and the reaction was carried out for 9 h. After the hydrolysis reaction was completed, the conical flask was cooled to room temperature, filtered with a Buchner funnel, and the hydrolysis residue was washed with deionized water until neutral, and the obtained filtrate was the bamboo shavings hemicellulose hydrolyzate.
[0025] The content of pentose polysaccharides in the bamboo chips is 20.13% according to the national standard GB / T2677.9-1994, and the content of pentoses in the hemicellulose hydrolyzate is 0.473 g tested by the phloroglucinol method. The pentose yield was calculated according to the f...
Embodiment 3
[0031] Weigh bamboo chips with an absolute dry weight of 2.4 g and a mesh number of 20 to 80 into a 250 mL ground conical flask, and add 0.1 mol / L according to the liquid-solid ratio of 40:1 (volume-to-mass ratio, in mL / g). The maleic acid solution was placed in a constant temperature heating magnetic stirrer at 130 °C, the magnetic stirring and condensation device were turned on, and the reaction was carried out for 7 h. After the hydrolysis reaction was completed, the conical flask was cooled to room temperature, filtered with a Buchner funnel, and the hydrolysis residue was washed with deionized water until neutral, and the obtained filtrate was the bamboo shavings hemicellulose hydrolyzate.
[0032]According to the national standard GB / T2677.9-1994, the content of pentoose in bamboo chips is 20.13%, and the content of pentose in hemicellulose hydrolyzate is 0.377 g by phloroglucinol method. The pentose yield was calculated according to the following formula. The hydrolyze...
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