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Preparation method of antialcoholism product

A product and inoculum technology, applied in microorganism-based methods, biochemical equipment and methods, functions of food ingredients, etc., can solve problems such as high cost and reduce cost, achieve less retention, improve metabolism, and sweetness. Moderate effect

Inactive Publication Date: 2017-02-15
TIANJIN TIDAI MEDICINE SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most anti-alcohol products are mainly made from plants, and the cost is relatively high. If you can directly extract acetaldehyde dehydrogenase from microorganisms or use microorganisms to make pharmaceuticals, the cost will be greatly reduced

Method used

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  • Preparation method of antialcoholism product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1) Strain cultivation: Inoculate Saccharomyces cerevisiae strains into a fermenter with strain medium, the liquid volume is 10%, the inoculum volume is 7%, the air volume is 1:0.6, pH 7, and the rotation speed is 200 rpm / min. The culture temperature was 28 ℃, cultured for 16 h, and the strain culture solution was obtained;

[0038] 2) Centrifuge the strain culture solution prepared in step 1), discard the supernatant to obtain a precipitate, wash twice with 0.066 mol / L disodium hydrogen phosphate buffer, keep the bacterial cells, add 5% 50 mM Tris -Cl (pH 8.0), 0.2 % 1 mM EDTA, 2 % 100 mM NaCl buffer solution, use Constant high-pressure cell disruptor to disrupt cells to obtain a solution containing alcohol dehydrogenase and acetaldehyde dehydrogenase;

[0039] 3) Preparation of corn husk enzymatic hydrolysis solution: After crushing corn husks, pass through a 60-mesh sieve, add purified water at a weight ratio of 1:8, adjust the pH value to 6.0, and stir at 120 rpm / min...

Embodiment 2

[0051] 1) Strain cultivation: Inoculate Saccharomyces cerevisiae strains into a fermenter with strain medium, the liquid volume is 10%, the inoculum volume is 7%, the air volume is 1:0.6, pH 7, and the rotation speed is 200 rpm / min. The culture temperature was 30 ℃, cultured for 16 h, and the strain culture solution was obtained;

[0052] 2) Centrifuge the strain culture solution prepared in step 1), discard the supernatant to obtain a precipitate, wash with 0.066 mol / L disodium hydrogen phosphate buffer 3 times, keep the bacterial cells, add 5% 50 mM Tris -Cl (pH 8.0), 0.2 % 1 mM EDTA, 2 % 100 mM NaCl buffer solution, use Constant high-pressure cell disruptor to disrupt cells to obtain a solution containing alcohol dehydrogenase and acetaldehyde dehydrogenase;

[0053] 3) Preparation of corn husk enzymatic hydrolysis solution: After crushing corn husks, pass through a 60-mesh sieve, add purified water at a weight ratio of 1:3, adjust the pH value to 5.0, and stir at 50 rpm / mi...

Embodiment 3

[0065] 1) Strain cultivation: Inoculate Saccharomyces cerevisiae strains into a fermenter with strain medium, the liquid volume is 10%, the inoculum volume is 7%, the air volume is 1:0.6, pH 7, and the rotation speed is 200 rpm / min. The culture temperature was 29 ℃, cultured for 16 h, and the strain culture solution was obtained;

[0066] 2) Centrifuge the strain culture solution prepared in step 1), discard the supernatant to obtain a precipitate, wash twice with 0.066 mol / L disodium hydrogen phosphate buffer, keep the bacterial cells, add 5% 50 mM Tris -Cl (pH 8.0), 0.2 % 1 mM EDTA, 2 % 100 mM NaCl buffer solution, use a constant high-pressure cell disruptor to disrupt cells to obtain a solution containing alcohol dehydrogenase and acetaldehyde dehydrogenase;

[0067] 3) Preparation of corn husk enzymatic hydrolysis solution: After crushing corn husks, pass through a 60-mesh sieve, add purified water at a weight ratio of 1:12, adjust the pH value to 5.5, stir at 80 rpm / min, ...

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Abstract

The invention relates to the technical field of foods, in particular to a preparation method of an antialcoholism product. The antialcoholism product comprises alcohol dehydrogenase and aldehyde dehydrogenase obtained through crushing saccharomyces cerevisiae cells, as well as L-arabinose obtained through fermentation of corn husk enzymatic hydrolysate. The product prepared by the method disclosed by the invention can be made into a product in any forms accepted by bromatology and health-care food science, and the made antialcoholism product has obvious effect of neutralizing the effect of alcoholic drinks.

Description

technical field [0001] The invention relates to the technical field of food, in particular to a preparation method of a hangover-relief product. Background technique [0002] my country is a country with a long history of wine culture, which has formed its unique style in the long development process, occupies a unique position, and penetrates into almost every field of social life. Wine has added rich colors to people's lives, and many people like to drink, or drink inevitably because of work needs. Moderate drinking has certain benefits to the human body. It can not only boost the mood, but also promote the blood circulation of the human body. But once excessive drinking, it will cause symptoms such as nausea, vomiting, memory loss, inability to concentrate, impaired motor ability, emotional instability, etc., and even death due to respiratory paralysis in severe cases. It has been shown that the increase in the incidence of esophageal cancer and liver cancer is also rel...

Claims

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Application Information

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IPC IPC(8): A23L33/125A23L33/10C12N9/04C12N9/02C12P19/02C12R1/865
CPCC12N9/0006A23V2002/00C12N9/0008C12P19/02C12P2203/00C12Y101/01001C12Y102/0101A23V2200/334A23V2250/082A23V2250/5118A23V2250/5114A23V2250/602A23V2250/032A23V2250/1614A23V2250/044A23V2250/246A23V2250/5086A23V2250/51082
Inventor 赵金梁赵发
Owner TIANJIN TIDAI MEDICINE SCI & TECH
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