Preparation method of antialcoholism product
A product and inoculum technology, applied in microorganism-based methods, biochemical equipment and methods, functions of food ingredients, etc., can solve problems such as high cost and reduce cost, achieve less retention, improve metabolism, and sweetness. Moderate effect
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[0036] Example 1
[0037] 1) Strain culture: Inoculate the Saccharomyces cerevisiae strains in a fermentation tank equipped with strain culture medium, with a liquid volume of 10%, an inoculum volume of 7%, air volume 1:0.6, pH 7, speed 200 rpm / min, Cultivation temperature is 28 ℃, culture for 16 h to obtain strain culture solution;
[0038] 2) Centrifuge the culture broth prepared in step 1), discard the supernatant to obtain a precipitate, wash twice with 0.066 mol / L disodium hydrogen phosphate buffer, retain the bacterial cells, and add Tris containing 5% 50 mM -cl (pH 8.0), 0.2% 1 mM EDTA, 2% 100 mM NaCl buffer, use Constant high-pressure cell crusher for cell disruption to obtain a solution containing alcohol dehydrogenase and acetaldehyde dehydrogenase;
[0039] 3) Preparation of corn husk enzymatic hydrolysis solution: corn husks are crushed and passed through a 60-mesh sieve. Purified water is added at a weight ratio of 1:8, pH is adjusted to 6.0, and the stirring speed is 1...
Example Embodiment
[0050] Example 2
[0051] 1) Strain culture: Inoculate the Saccharomyces cerevisiae strains in a fermentation tank equipped with strain culture medium, with a liquid volume of 10%, an inoculum volume of 7%, air volume 1:0.6, pH 7, speed 200 rpm / min, Cultivation temperature is 30 ℃, culture for 16 h to obtain strain culture solution;
[0052] 2) Centrifuge the culture broth prepared in step 1), discard the supernatant to obtain the precipitate, wash 3 times with 0.066 mol / L disodium hydrogen phosphate buffer, save the bacterial cells, and add Tris containing 5% 50 mM -cl (pH 8.0), 0.2% 1 mM EDTA, 2% 100 mM NaCl buffer, use Constant high-pressure cell crusher for cell disruption to obtain a solution containing alcohol dehydrogenase and acetaldehyde dehydrogenase;
[0053] 3) Preparation of corn husk enzymatic hydrolysate: corn husks are crushed and passed through a 60-mesh sieve. Purified water is added at a weight ratio of 1:3, pH is adjusted to 5.0, and the stirring speed is 50 rpm / ...
Example Embodiment
[0064] Example 3
[0065] 1) Strain culture: Inoculate the Saccharomyces cerevisiae strains in a fermentation tank equipped with strain culture medium, with a liquid volume of 10%, an inoculum volume of 7%, air volume 1:0.6, pH 7, speed 200 rpm / min, Cultivation temperature is 29 ℃, culture for 16 h to obtain strain culture solution;
[0066] 2) Centrifuge the culture broth prepared in step 1), discard the supernatant to obtain a precipitate, wash twice with 0.066 mol / L disodium hydrogen phosphate buffer, retain the bacterial cells, and add Tris containing 5% 50 mM -cl (pH 8.0), 0.2% 1 mM EDTA, 2% 100 mM NaCl buffer, use a constant high-pressure cell crusher for cell disruption to obtain a solution containing alcohol dehydrogenase and acetaldehyde dehydrogenase;
[0067] 3) Preparation of corn husk enzymatic hydrolysis solution: corn husks are crushed and passed through a 60-mesh sieve. Purified water is added at a weight ratio of 1:12, pH is adjusted to 5.5, and the stirring speed i...
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