Mixed hyaluronic-acid gel and preparation method thereof

A technology of hyaluronic acid and sodium hyaluronate, which is used in pharmaceutical formulations, medical science, prostheses, etc., can solve the problem of not guaranteeing the retention time of hyaluronic acid, and achieve excellent rheological properties, good mechanical properties, and network Strong and stable structure

Active Publication Date: 2017-02-15
SHAANXI BIO REGENERATIVE MEDICINE CO LTD
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  • Application Information

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Problems solved by technology

[0005] The present invention provides a mixed hyaluronic acid gel and its preparation method to solve the problem that hyaluronic acid fillers or gels in the prior art can not only ensure the retention time of hyaluronic acid in the body, but also make cell growth factors effective Promoting the growth of the body's own collagen fibers

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  • Mixed hyaluronic-acid gel and preparation method thereof
  • Mixed hyaluronic-acid gel and preparation method thereof
  • Mixed hyaluronic-acid gel and preparation method thereof

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[0034] An embodiment of the present invention provides a method for preparing a mixed hyaluronic acid gel, the method comprising:

[0035] S1, reacting sodium hyaluronate with a cross-linking agent at a pH value of 9-13 to obtain a cross-linked sodium hyaluronate gel;

[0036] S2, reacting the polynucleotide with a cross-linking agent under the condition of a pH value of 2-5 to obtain a cross-linked nucleotide gel;

[0037] S3. After mixing the cross-linked sodium hyaluronate gel and the cross-linked nucleotide gel, react with the cross-linking agent again, adjust the pH value to neutral, and prepare a mixed transparent Hyaluronic Acid Gel.

[0038] Wherein, the implementation of step S1 and step S2 is not limited in order and can be performed at the same time, which can shorten the process cycle and effectively improve production efficiency.

[0039] In practice, hyaluronic acid usually exists in the form of hyaluronate, such as sodium salt, potassium salt, etc., and sodium...

Embodiment 1

[0066] (1) Dissolve 5.0g of sodium hyaluronate in 50mL of NaOH solution with a pH value of 12, stir evenly, add 0.03g of 1,4-butanediol diglycidyl ether cross-linking agent, mix well, and place in a water bath at 40°C After 3 hours, a cross-linked sodium hyaluronate gel was obtained; wherein, the molecular weight of sodium hyaluronate was 2 million Daltons;

[0067] (2) Dissolve 5.0g of polynucleotide in the HCl solution with a pH value of 3, stir evenly, add 0.025g of 1,4-butanediol diglycidyl ether cross-linking agent, mix evenly, and place at 30°C Submerged in a water bath for 6 hours to obtain a cross-linked nucleotide gel; wherein, the molecular weight of the polynucleotide is 500 Daltons;

[0068] (3) Mix 50 g of cross-linked sodium hyaluronate gel and cross-linked nucleotide gel, adjust the pH to 3-5, add 0.05 g of 1,4-butanediol diglycidyl ether cross-linking agent, and mix After uniformity, put it in a water bath at 30°C for 6 hours, then adjust the pH to 7.0 with so...

Embodiment 2

[0072] (1) Dissolve 5.0g of sodium hyaluronate in 50mL of NaOH solution with a pH value of 13, stir evenly, add 0.075g of 1,4-butanediol diglycidyl ether cross-linking agent, mix well, and place at 37°C Submerged in a water bath for 12 hours to obtain a cross-linked sodium hyaluronate gel; wherein, the molecular weight of sodium hyaluronate is 2 million Daltons;

[0073] (2) Dissolve 3.0g polynucleotide in HCl solution with pH value of 4, stir well, add 0.006g 1,4-butanediol diglycidyl ether cross-linking agent, mix well and place at 40°C Submerged in a water bath for 3 hours to obtain a cross-linked nucleotide gel; wherein, the molecular weight of the polynucleotide is 10,000 Daltons;

[0074] (3) Take 50 g of cross-linked sodium hyaluronate gel, and then take 20 g of cross-linked nucleotide gel for mixing, adjust the pH to 3-5, add 0.035 g of 1,4-butanediol diglycidyl ether cross-linking agent , after mixing evenly, place in a water bath at 30°C for 6 hours, then adjust the...

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Abstract

The invention provides mixed hyaluronic-acid gel and a preparation method thereof, wherein the method comprises the following steps of making sodium hyaluronate react with a cross-linking agent in a condition that a pH (potential of Hydrogen) value is 9 to 13, so as to obtain cross-linked sodium-hyaluronate gel; making polynucleotide react with the cross-linking agent in a condition that a pH value is 2 to 5, so as to obtain cross-linked nucleotide gel; mixing the cross-linked sodium-hyaluronate gel with the cross-linked nucleotide gel, then making an obtained mixture react with the cross-linking agent once again, and finally preparing to obtain the mixed hyaluronic-acid gel. According to the mixed hyaluronic-acid gel and the preparation method thereof, through two cross linking reactions, the problems that a hyaluronic acid type filling agent or gel in the prior art cannot be used for not only guaranteeing the in-vivo persistence time of hyaluronic acid, but also enabling a cell growth factor to effectively promote the growth of the self collagenous fiber of an organism are solved.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a mixed hyaluronic acid gel and a preparation method thereof. Background technique [0002] Hyaluronic acid (HA for short), also known as hyaluronic acid, is a natural hyaluronic acid that is a physiological substance that is mostly used to fill the space supported by cells and collagen fibers, and has excellent water-locking and water-retaining functions. With the increase of age, the influence of external force extrusion traction and light radiation and other factors, the hyaluronic acid in the human skin is gradually lost, which in turn causes the reduction of dermal cells, collagen and elastic fibers and the collapse of the three-dimensional space of the parts, leading to skin relaxation , Facial wrinkles appear. This problem can be effectively solved by injecting hyaluronic acid, but due to reasons such as hyaluronidase and free radical degradation, exogenous n...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/24C08J3/075A61L27/26A61L27/52A61L27/54A61L27/18A61L27/20
CPCA61L27/26A61L27/52A61L27/54A61L2300/402A61L2300/412A61L2400/06C08J3/075C08J3/24C08J2305/08C08L5/08
Inventor 张海龙乔宝坤张勇杰黄文涛宋坤
Owner SHAANXI BIO REGENERATIVE MEDICINE CO LTD
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