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A method for fermenting and cultivating anaerobic bacteria based on magnetic response pickering emulsion

A fermentation culture, magnetic response technology, applied in biochemical equipment and methods, microorganisms, microorganisms, etc., can solve the problems of high input cost, unfavorable small amount of anaerobic bacteria culture, bacterial transfer and other problems, and achieves strong practicability and long system. time-steady effect

Active Publication Date: 2019-12-20
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These two culture methods have high input costs and consume a lot of resources, which are not conducive to the cultivation of a small amount of anaerobic bacteria
This limits the research on anaerobic bacteria to a certain extent.
In addition, for the transfer of anaerobic bacteria, it is still difficult to achieve the transfer of bacteria under absolute anaerobic conditions.

Method used

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  • A method for fermenting and cultivating anaerobic bacteria based on magnetic response pickering emulsion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] According to the growth habit of lactic acid bacteria, the culture medium was prepared with 0.5wt% peptone, 0.5wt% glucose, 0.5wt% NaCl and 98.5wt% water (mass percentage) as raw materials and sterilized at 121° C. for 20 minutes. Inject 1mL of the original strain into 50mL of the sterilized culture medium, and mix well to form a lactic acid bacteria liquid for use.

[0023] Prepare 60 mL of ethanol / water solution with a volume ratio of 1:1, and weigh 1 g of commercially available Fe 3 o 4 Nanoparticles were added to the above ethanol / water solution, and ultrasonically treated at 25° C. and 40 KHz for 20 minutes. Fe to be ultrasonically dispersed 3 o 4 After the nanoparticles had naturally settled, the supernatant was removed. Add ethanol / water solution and repeat the above-mentioned washing operation 3 times, after the washing is completed, the Fe 3 o 4 Add 30mL of absolute ethanol to the nanoparticle precipitation, and disperse with electric stirring for 30 minu...

Embodiment 2

[0026] Embodiment two: in (a) step, Fe 3 o 4Nanoparticle precipitation: dehydrated alcohol: dodecyltrichlorosilane: water=1:24:1:30 (mass ratio), in (b) step, bacteria liquid: n-octane=8:5 (mass ratio) Other production conditions are as embodiment one.

Embodiment 3

[0027] Embodiment three: in (a) step, Fe 3 o 4 Nanoparticle precipitation: dehydrated alcohol: octadecyltrichlorosilane: water=1:24:2:30 (mass ratio), in (b) step, bacterial liquid: white oil=8:11 (mass ratio) other The production conditions are as in Example 1.

[0028] It can be seen from the above examples that the anaerobic bacteria fermentation system based on the magnetic response Pickering emulsion of the present invention can realize the cultivation and directional transfer of anaerobic bacteria, and has broad application prospects.

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Abstract

The invention discloses a method for fermenting and cultivating anaerobic bacteria based on a magnetic response Pickering emulsion. The anaerobic bacteria solution is used as the water phase, n-octane or white oil is used as the oil phase, and the alkylsilane-modified Fe 3 o 4 Nanoparticles are used as stabilizers, and the emulsion forms a magnetically responsive Pickering emulsion; the Pickering emulsion is used as a micro-biological reaction system to realize the cultivation of anaerobic bacteria. During the cultivation process, an absolute anaerobic environment is provided through the water-in-oil emulsion, and the anaerobic culture system is directed to move to different physical environments with the help of an external magnetic field for fermentation, or migration concentration, or separation, selection and enrichment. After the fermentation is over, the Pickering emulsion enters the water phase from the oil-water interface layer and breaks through the attraction of the magnet, and the oil phase and the water phase (bacterial liquid and metabolites) are separated by gravity. The invention has the advantages of simple operation, low input cost and resource saving, and has a good application prospect in the industrial application of anaerobic fermentation culture.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation. Specifically, it relates to an anaerobic fermentation culture technology. Background technique [0002] Because anaerobic bacteria need to grow under anaerobic conditions, it is difficult to cultivate anaerobic bacteria in the laboratory. The most commonly used anaerobic culture methods include anaerobic incubator and anaerobic bag culture. However, when using an anaerobic box, specific gases such as hydrogen, nitrogen, and carbon dioxide must be purchased to exchange the air in the anaerobic box for anaerobic purposes, while the anaerobic bag is a one-time application. These two culture methods have high input costs and consume a lot of resources, which are not conducive to the cultivation of a small amount of anaerobic bacteria. Thus, the research on anaerobic bacteria is limited to a certain extent. In addition, for the transfer of anaerobic bacteria, it is still difficult t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/00
CPCC12N1/00
Inventor 孟涛张家琪宋婧谢春燕郭婷
Owner SOUTHWEST JIAOTONG UNIV
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