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Microbial mixed flora for fermenting to produce 1,3-propanediol and fermentation method

A technology of mixed flora and fermentation method, applied in the field of bioengineering, can solve the problems of unverified biological safety, inhibition of bacterial growth and metabolism, unknown composition of bacterial species, etc., and achieves strong tolerance, easy operation, and reduced production costs. Effect

Active Publication Date: 2017-02-15
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when the substrate crude glycerol concentration reaches 70g / L, the growth and metabolism of the bacteria will be significantly inhibited. At the same time, the composition of the bacteria is unknown, and the biological safety needs to be verified.

Method used

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  • Microbial mixed flora for fermenting to produce 1,3-propanediol and fermentation method
  • Microbial mixed flora for fermenting to produce 1,3-propanediol and fermentation method
  • Microbial mixed flora for fermenting to produce 1,3-propanediol and fermentation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Screening of microbial flora domesticated to produce 1,3-propanediol from anaerobic activated sludge

[0043] Take 2.0g of anaerobic activated sludge, insert it into the seed medium containing 22g / L crude glycerol, and culture it anaerobically under the conditions of 37°C and 200r / min, and gradually reduce the inoculation amount for subculture after the glycerol in the medium is exhausted. After 20 subcultures, the biomass and metabolism of the flora were basically stable. Natural enrichment and domestication processes such as figure 1 and 2 As shown, the overall can be divided into three stages: initial stage (2nd generation to 4th generation), fluctuation period (5th generation to 12th generation) and stable period (after 12th generation). The initial stage of screening is the stage when the flora structure adapts to the culture environment. It can enrich the strains that can use glycerol and grow rapidly, and eliminate the strains that cannot use substrat...

Embodiment 2

[0047] Example 2 Tolerance of anaerobic microbial flora C2-2M to crude glycerol

[0048] After the mixed flora C2-2M obtained in Example 1 is cultivated in the seed medium, it is inoculated into a 5L fermenter with 2L fermentation medium by 10% volume ratio to cultivate, and the initial glycerol concentration of each batch of fermentation is changed from 40g / L is gradually increased to 140g / L (adding an appropriate amount of crude glycerin to prepare a fermentation medium with an initial glycerin concentration of 40-140g / L), as image 3 shown. The fermentation results show that the microbial flora C2-2M has a strong tolerance to crude glycerol, and can grow and metabolize in crude glycerol with a glycerol content of 120g / L. The concentration of 1,3-propanediol reaches 60.61g / L, and the molar conversion The rate is 0.63mol / mol, and the production intensity is 3.79g / (L·h). Relatively speaking, the batch fermentation efficiency is higher when the initial glycerol concentration...

Embodiment 3

[0049] Example 3 Batch fermentation of anaerobic bacteria S1 with crude glycerol as substrate

[0050] The fermentation results of single bacterium S1 using 80g / L crude glycerol as substrate are as follows: Figure 5 As shown, the concentration of 1,3-propanediol is 44.34g / L, the molar conversion rate is 0.67mol / mol, and the production intensity is 0.92g / (L·h). Although the concentration of 1,3-propanediol and the molar conversion rate of crude glycerol fermented by single bacteria S1 are similar to the fermentation level of microbial flora C2-2M, the fermentation time is 38 hours longer than that of microbial flora fermentation, and the production intensity is only for microbial flora fermentation of 22.4%. This indicated that the microbial flora C2-2M had better fermentation performance than the single bacteria, could tolerate higher glycerol concentration, and the fermentation time was shorter.

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Abstract

The invention provides a microbial mixed flora used for fermenting to produce 1,3-propanediol by using glycerin as a substrate and a fermentation method. The microbial flora mainly contains clostridium butyricum (95 percent or above), and in addition, contains mixed symbiotic bacteria (5 percent or below) of a clostridium bacterial strain and a lactobacillus bacterial strain; the proportion of the effective viable counts of the clostridium bacterial strain and the lactobacillus bacterial strain in the mixed symbiotic bacteria is (1.50 to 4.99) : (0.01 to 0.05). In comparison with the fermentation of a single bacterium, the microbial mixed flora is good in fermentation stability, is high in biological safety, and has the advantages that the tolerance of the crude glycerin substrate is high, the production intensity is high, the sterilization is not needed in a production process, and the like; a novel fermentation mode is provided for the industrial production of the 1,3-propanediol. In the batch type fed-batch fermentation in which the rough glycerin is used as the substrate, the concentration of the 1,3-propanediol can reach 79.78g / L; the molar conversion ratio is 0.65mol / mol; the production intensity is 3.32g / (L*h).

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for producing 1,3-propanediol by fermenting crude glycerol as a substrate through anaerobic microbial flora. Background technique [0002] 1,3-Propanediol is an important chemical platform compound, which is widely used in many industries such as medicine, food and cosmetics. PTF) monomer. As a new type of polyester fiber, PTT has excellent softness, resilience and dyeability, and has a good development prospect in the textile industry. PTF is a 100% renewable new type of polyester, which is an ideal raw material to replace plastic products and has great application potential in the packaging industry. Therefore, 1,3-propanediol has a huge market demand space. [0003] The main production methods of 1,3-propanediol are chemical method and biological method. Chemical methods include acrolein hydration hydrogenation method and ethylene oxide carbonyl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P7/18C12R1/145C12R1/225
CPCC12N1/20C12P7/18
Inventor 修志龙周瑾洁姜莉莉沈俊涛孙亚琴戴建英牟英
Owner DALIAN UNIV OF TECH
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