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Spraying system based on matrix-assisted laser desorption ionization mass spectrometry imaging, and biological sample analysis method

A matrix-assisted laser and spray system technology, applied in the field of spray system and analysis of biological samples, can solve the problems of harsh reagent and matrix selection conditions, difficult to meet the needs of daily experiments, expensive equipment purchase, etc. Controllable effect

Active Publication Date: 2017-02-15
维科托(北京)科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these devices are very expensive to purchase, costing 40,000 to 100,000 US dollars, and the operating requirements, reagents and matrix selection conditions are relatively harsh, which is difficult to meet the needs of daily experiments

Method used

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  • Spraying system based on matrix-assisted laser desorption ionization mass spectrometry imaging, and biological sample analysis method
  • Spraying system based on matrix-assisted laser desorption ionization mass spectrometry imaging, and biological sample analysis method
  • Spraying system based on matrix-assisted laser desorption ionization mass spectrometry imaging, and biological sample analysis method

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0081] Determination of the distance between the lower end of the capillary and the sample stage:

[0082] Experimental conditions: the biological tissue samples are normal mouse brain tissue slices, the matrix is ​​2,5-dihydroxybenzoic acid (DHB), the sheath gas (nitrogen) flow rate is 50L / h, and the volume ratio of acetonitrile to water in the acetonitrile solution is 8: 2. The concentration of the matrix solution is 30mg / mL, the flow rate of the matrix solution is 250μL / h, the voltage of the power supply device is 4500V, the spraying time is 12min, and the distances between the lower end of the capillary and the sample stage are 4.5cm, 6.5cm, 8.5cm and 10.5cm.

[0083] Operation steps: first place the ITO glass with the mouse brain tissue slice on the sample stage, turn on the sample stage control device, so that the sliced ​​sample can move back and forth at a uniform speed in a specific two-dimensional interval, and then adjust the distance between the sample stage and th...

experiment example 2

[0086] Effect of high voltage environment on the quality of matrix crystals:

[0087] Experimental conditions: the biological tissue samples are normal mouse brain tissue slices, the matrix is ​​2,5-dihydroxybenzoic acid (DHB), the sheath gas (nitrogen) flow rate is 50L / h, and the volume ratio of acetonitrile to water in the acetonitrile solution is 8: 2. The concentration of the matrix solution is 30mg / mL, the flow rate of the matrix solution is 250μL / h, the spraying time is 12min, the distance between the lower end of the capillary and the sample stage is 8.5cm, and the voltage of the power supply device is 0V.

[0088] Operation steps: the same as Experimental Example 1, the micrographs of the obtained matrix crystals are respectively as follows Figure 6 shown.

[0089] Depend on Figure 6 It can be seen that under the same conditions, the crystal size of DHB obtained by turning off the high voltage is significantly larger than that of the crystal with high voltage ( F...

experiment example 3

[0091] Effect of matrix solution flow rate on matrix crystal quality:

[0092] Experimental conditions: the biological tissue samples are normal mouse brain tissue slices, the matrix is ​​2,5-dihydroxybenzoic acid (DHB), the sheath gas (nitrogen) flow rate is 50L / h, and the volume ratio of acetonitrile to water in the acetonitrile solution is 8: 2. The concentration of the matrix solution is 30mg / mL, the voltage of the power supply device is 4500V, the spraying time is 12min, the distance between the lower end of the capillary and the sample stage is 8.5cm, the flow rate of the matrix solution is 125μL / h, the obtained matrix crystal micrograph Respectively as Figure 7 shown.

[0093] Operation steps: the same as Experimental Example 1, the micrographs of the obtained matrix crystals are respectively as follows Figure 7 shown.

[0094] Depend on Figure 7 It can be seen that when the flow rate of the matrix solution is 125 μL / h, compared with the flow rate of the matrix s...

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Abstract

The present invention discloses a spraying system based on matrix-assisted laser desorption ionization mass spectrometry imaging, and a biological sample analysis method. The spraying system comprises: an injection device for supplying a matrix solution; a capillary tube connected to the injection device and used for supplying the matrix solution to the capillary tube; a power supply device connected to the capillary tube and used for atomizing the matrix solution in the capillary tube so as to obtain matrix solution mist droplets; a sheath gas pipe arranged on the capillary tube in a sleeved manner; a sheath gas supply device connected to the sheath gas pipe and used for performing spray coating on the matrix solution mist droplets by using the sheath gas; a sample table movably arranged on the lower end of the capillary tube; and a sample table control device connected to the sample table and used for controlling the moving of the sample table. According to the present invention, with the system, the uniform spray coating of the matrix on the biological sample surface can be achieved; and the system has advantages of simple structure, cheap and easily available fittings, and strong controllability.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular, the invention relates to a spray system for matrix-assisted laser desorption ionization mass spectrometry imaging and a method for analyzing biological samples. Background technique [0002] Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) is a technique that enables region-specific molecular detection directly from biological samples. At present, this technology has been widely used in the qualitative, quantitative, in situ analysis of proteins and lipids in biological tissue slices, the exploration of biomarkers, the study of disease mechanisms, etc., and is playing an increasingly important role in the fields of biochemistry and clinical medicine. increasingly important role. [0003] In the process of matrix-assisted laser desorption ionization mass spectrometry imaging, sample preparation is a very important link, and the spraying of the matrix on...

Claims

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Application Information

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IPC IPC(8): G01N27/64
Inventor 赵镇文李石磊张阳阳魏妍波
Owner 维科托(北京)科技有限公司