Method for avoiding appearance of laying hen type phenotypic offspring caused by hybridization of white-feather Xuefeng black-bone chicken and jute-feather Xuefeng black bone chicken

A technology of Xuefeng black-boned chicken and Xuefengwu, which is applied in the field of molecular genetics, can solve the problems of error-prone, large workload, low accuracy, etc., and achieves the effect of less error-prone, less links and fast speed.

Pending Publication Date: 2017-02-22
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] (1) The cycle is long, and it takes at least 3 months from hatching to being able to judge the feather color;
[0005] (2) The workload is heavy, and the eggs of each hen must be clearly marked;
[0006] (3) The process is cumbersome and error-prone. First, the mother of each egg needs to be recorded in the laying house, and then the eggs need to be hatched in groups in the incubator. Phenotype identification of the chickens is carried out around the time to confirm the hens that need to be eliminated, and finally the hens are eliminated;
[0007] (4) The accuracy rate is less than 100%
Since it is a test cross method, the results cannot be guaranteed to be 100% accurate

Method used

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  • Method for avoiding appearance of laying hen type phenotypic offspring caused by hybridization of white-feather Xuefeng black-bone chicken and jute-feather Xuefeng black bone chicken

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1. Collection of individual samples: 48 white-feathered and jute-feathered offspring, including 24 layer-like phenotype individuals and 24 jute-feathered individuals. Draw about 1 mL of blood from the vein under the chicken wing with a disposable syringe, inject it into a 1.5 mL centrifuge tube that has been autoclaved and filled with about 200 μL of 2% sterile EDTA (Ethylenediaminetetraacetic acid, EDTA) anticoagulant, shake it gently, and record Wing number, stored at -20°C for later use.

[0026] 2. DNA extraction of the sample to be tested: the extraction of genomic DNA adopts the phenol-chloroform extraction method (Osper F et al., 1998):

[0027] (1) Put 30 μL of whole blood into a 1.5 mL centrifuge tube, add 470 μL of 1×SET buffer, 12.5 μL of 20% SDS and 6 μL of 10 mg / mL proteinase K, mix well and place in a 55°C water bath overnight;

[0028] (2) Take out the sample in a 1.5mL centrifuge tube, add 500μL saturated phenol, shake gently for 20min, and centrifuge a...

Embodiment 2

[0048] Example 2: Excluding Xuefeng Silky White Feather Individuals Carrying Dominant White Mutation

[0049] A method for avoiding laying hen-like phenotype offspring from the hybridization of white Yuxuefeng silkies and jute-colored Yuxuefeng silkies, the main key point of which is to eliminate individuals carrying dominant white mutations from white-feather Xuefeng silkies , to form a new white-feather line, to ensure that the offspring of the newly established white-feather line of Xuefeng black-bone chicken and jute chicken will not appear layer-like phenotypes. The specific method steps are as follows:

[0050] 1. Sample collection: Collect samples from all individuals of Xuefeng black-bone chicken of the white feather line in a certain enterprise. Method is the same as step 1 in embodiment 1.

[0051] 2. DNA extraction: DNA extraction from the blood sample of the individual to be examined is the same as Step 2 in Example 1.

[0052] 3. Genotyping of the individual to...

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Abstract

The invention discloses a method for avoiding the appearance of a laying hen type phenotypic offspring caused by the hybridization of white-feather Xuefeng black-bone chicken and jute-feather Xuefeng black bone chicken. The method comprises the following steps: eliminating individuals carrying dominant white mutations, namely genotypes II and Ii in the white-feather Xuefeng black-bone chicken and hybridizing with the jute-feather Xuefeng black bone chicken. The laying hen type phenotypic individuals cannot appear in the hybrid offspring of a newly-established white-feather pure line and jute-feather pure line. The method disclosed by the invention is of important significance in eliminating unqualified white-feather individuals produced and identified by an enterprise and breeding the white-feather Xuefeng black-bone chicken meeting the requirements.

Description

technical field [0001] The invention relates to the field of molecular genetics, and in particular relates to a method for avoiding laying hen-like phenotype offspring from crossing white Yuxuefeng silkies and jute-colored Yuxuefeng silkies. Background technique [0002] Xuefeng black-bone chicken has high nutritional value, the crude protein content of dry matter is more than 76%, the meat is tender and delicious, which is closely related to the high content of aspartic acid, glutamic acid and lysine, and is rich in human body A variety of essential amino acids, vitamins and trace elements. It can regulate the body's metabolism and endocrine, increase human red blood cells and hemoglobin, and has the functions of anti-fatigue, regulating physiology, enhancing immunity, and improving hypoxia resistance. [0003] Most offspring of Xuefeng black-bone chicken white-feather line and jute-feather line are jute feathers, but there will be layer-like phenotypes, which do not meet ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02
CPCA01K67/02
Inventor 许继国聂庆华高鑫凤叶峭王敬友张细权
Owner SOUTH CHINA AGRI UNIV
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