Preparation method of mouse skin burn model

A mouse and model technology, applied in the fields of medical science, veterinary instruments, veterinary surgery, etc., can solve the problem of precise control and repeated repetition of unfavorable burn depth, burn wound area cannot be accurately controlled, and burn area and depth are difficult. To achieve the effects of reducing mortality and anesthesia effects, avoiding pathophysiological changes, and accurately controlling the area and location of burns

Inactive Publication Date: 2017-02-22
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the past, the mouse burn model was mainly prepared by hot water bath or flame burning, the burn area and depth were difficult to control, and the mice needed to be deeply anesthetized, the mortality rate of the mice was high, and the repeatability of the experiment was poor.
In recent years, with the progress of laser technology, Liu Hanqing et al. reported the application of laser-semiconductor laser or helium-neon laser to irradiate animal skin to prepare burn models (see Chinese invention patent application "Method for making a burn animal model", the publication number is CN105816256A) However, the burn model produced by this method has the following disadvantages: ①The area of ​​the burn wound cannot be accurately controlled; ②The control of the depth of the burn is mainly through controlling the laser ablation time rather than controlling the energy of ablation, which is controlled by the operator's manual response. The speed limit is not conducive to the precise control of the burn depth and multiple repetitions; ③The laser irradiation of the burn model needs 45-240S, which brings great pain to the animals during the process, and requires deep anesthesia for the animals, which may easily lead to pathology in experimental animals Physiological changes and even death
[0005] So far, there is no mouse skin burn model with safe operation, good reproducibility, and precise control of burn area and depth.

Method used

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  • Preparation method of mouse skin burn model
  • Preparation method of mouse skin burn model
  • Preparation method of mouse skin burn model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1. Preparations before making models such as mouse anesthesia and depilation

[0041] 1. Mice anesthetized

[0042] The operator pinches the skin of the neck and back of the mouse with his left hand to fix the mouse and expose the abdomen, wipes the alcohol cotton ball twice at the place where the needle is to be inserted in the abdomen of the mouse, and injects 0.15ml of 1% pentobar into the abdomen of the mouse with a 1ml syringe in his right hand Sodium perbital, after the injection, put the mouse into the cage until the anesthesia takes effect.

[0043] 2. Mouse depilation

[0044] After the mice were anesthetized, a 3×3cm area on the back was designed to be depilated, and the depilatory cream was applied to the set area, and the back hair was removed after 3 minutes.

Embodiment 2

[0045] Example 2. Carbon dioxide fractional laser to make a mouse burn model

[0046]Using a carbon dioxide fractional laser system (UltraPulse Encore, Lumenis, Yokneam, Israel), adjust to DEEP FX mode, 300Hz, density 25%, pulse number 1 time, adjust the laser energy to 2.5, 5, 10, 20, 30, 40mJ, the laser irradiation distance is determined by the laser head, the red semiconductor laser is used as the guiding beam for pulses, and six 0.5×0.5 wounds are respectively made on the back of the mouse ( figure 1 and figure 2 ).

Embodiment 3

[0047] The mouse burn model of embodiment 3. making is generally photographed and H&E staining

[0048] Place the modeled mice flat on the table, and take pictures after exposing the wound. Then the damaged skin and surrounding normal skin were cut out, and the hot water scald model was made according to previous literature reports, and the skin was cut out as a control (see literature: Ren Peng, Guan Dawei, Zhao Rui, Ma Wenxiang, Zhang Shutao, Establishment of mouse skin scald model, Journal of Forensic Science, 2012, Vol. 28, No. 2, pp. 92-94, 4 pages in total). H&E staining (see literature for methods: Zhang F, Wang X, Qiu X, Wang J, Fang H, Wang Z, Sun Y, Xia Z. The protective effect of Esculentoside A on experimental acute liver injury in mice. PLoS One.2014Nov 18; 9 (11):e113107.) The results show that fixed pulse distance, time and frequency, and changing pulse energy can respectively create I°, shallow II°, deep II°, III° and IV° wounds ( image 3 , 4 and Table 1). ...

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Abstract

The invention relates to the technical field of fundamental research on burns and experimental animal models and provides a preparation method of a mouse skin burn model, comprising the specific step of preparing the mouse skin burn model by using exfoliative carbon dioxide fractional laser technology; burn wound area and depth can be precisely controlled during modeling, modeling time is short, a mouse is under slight anesthesia, and model making success rate and repetitive rate are high; the mouse skin burn model is expected to act as an important animal model in fundamental researches on burns.

Description

technical field [0001] The invention relates to the technical fields of burn basic research and experimental animal models, and relates to a method for preparing a mouse skin burn model by using exfoliative carbon dioxide fractional laser technology. Background technique [0002] In recent years, the level of burn treatment has made many breakthroughs and remarkable achievements in the fields of clinical treatment and basic research. However, there are still many major clinical problems that need to be explored and solved urgently in the field of modern burn surgery. It is urgent to strengthen relevant basic research, such as: the mechanism of skin pathological changes in severe burns, wound repair after burns, and the protection of important organs after burns Wait. [0003] In the process of clinical and basic research, issues such as ethics and research funding have restricted some burn research in patients or large mammals to a certain extent. Therefore, the preparatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D7/00A61D1/00
CPCA61D7/00A61D1/00
Inventor 张放吕开阳夏照帆肖永强朱世辉刘善荣
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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