A bovine serum albumin-multifunctional factor complex
A technology of bovine serum albumin and multifunctional factors, which is applied in the field of polymer chemistry and functional food, and achieves the effects of low cost, enhanced storage stability, and simple method
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Embodiment 1
[0023] Embodiment 1 The effect of retinol or EGCG on the combination of bovine serum albumin and resveratrol
[0024] (1) Add bovine serum albumin solution and resveratrol solution sequentially into 5-20 mM phosphate buffer solution, adjust with buffer solution so that the concentrations of bovine serum albumin and resveratrol are 2 μM and 10 μM respectively;
[0025] (2) Add bovine serum albumin solution, retinol solution and resveratrol solution in sequence in 5-20mM phosphate buffer solution, wherein the interval between adding retinol solution and resveratrol solution is 30 minutes, use buffer The solution was adjusted so that the concentration of bovine serum albumin was 2 μM, the concentration of retinol was 5 μM, and the concentration of resveratrol was 10 μM;
[0026] (3) Add bovine serum albumin solution, EGCG solution and resveratrol solution in sequence in 5-20mM phosphate buffer solution, wherein the interval between adding EGCG solution and resveratrol solution is...
Embodiment 2
[0029] Example 2 Effects of resveratrol or EGCG on the binding of bovine serum albumin and retinol
[0030] (1) Add bovine serum albumin solution and retinol solution sequentially to 5-20 mM phosphate buffer solution, adjust with buffer solution so that the concentrations of bovine serum albumin and retinol are 2 μM and 10 μM, respectively;
[0031] (2) Add bovine serum albumin solution, resveratrol solution and retinol solution in sequence in 5-20mM phosphate buffer solution, wherein the interval between adding resveratrol solution and retinol solution is 30 minutes, use buffer The solution was adjusted so that the concentration of bovine serum albumin was 2 μM, the concentration of resveratrol was 5 μM, and the concentration of retinol was 10 μM;
[0032] (3) Add bovine serum albumin solution, EGCG solution and retinol solution in sequence in 5-20mM phosphate buffer solution, wherein the interval between adding EGCG solution and retinol solution is 30 minutes, adjust with bu...
Embodiment 3
[0035] Embodiment 3 Resveratrol or retinol are on the impact of bovine serum albumin and EGCG binding
[0036] (1) Add bovine serum albumin solution and EGCG solution sequentially into 5-20 mM phosphate buffer solution, adjust with buffer solution so that the concentration of bovine serum albumin is 2 μM, and the concentration of EGCG is 10 μM;
[0037] (2) Add bovine serum albumin solution, resveratrol solution and EGCG solution sequentially in 5-20mM phosphate buffer solution, wherein the interval between resveratrol solution and EGCG solution is 30 minutes, adjust with buffer solution so that bovine The concentration of serum albumin was 2 μM, the concentration of resveratrol was 5 μM, and the concentration of EGCG was 10 μM;
[0038] (3) Add bovine serum albumin solution, retinol solution and EGCG solution sequentially in 5-20mM phosphate buffer solution, wherein the interval between adding retinol solution and EGCG solution is 30 minutes, adjust with buffer to make bovine...
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