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Method for improving Haematococcus pluvialis to produce astaxanthin by using alkali pretreatment technology

A Haematococcus pluvialis and alkali pretreatment technology, applied in the biological field, can solve problems such as poor tolerance, sharp drop in astaxanthin production, and inability to induce culture, so as to improve environmental tolerance and avoid cell activity decline , The effect of shortening the harvesting time

Active Publication Date: 2017-02-22
BIOALGO CY CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] During the two-step culture method, it is necessary to concentrate and harvest the green vegetative cells cultivated in the first step. The traditional natural sedimentation has the disadvantages of slow sedimentation, incomplete sedimentation, large loss of algae cells, and reduced cell activity. However, centrifugal separation High energy consumption, the method of adding flocculant can not realize the separation of algae cells and flocculant, can not be induced culture
In addition, due to the poor tolerance of green algae cells to unfavorable environments, they are easily polluted by invading organisms. In the early stage of the second induction step, drastic changes in the external environment (high light, high salt, high temperature, etc.) Kinetic cells) are difficult to adapt, drifting, autolysis and other phenomena appear rapidly, and the dead cells provide nutrients for the invading organisms in the algae liquid, which will further affect the growth of healthy cells, and eventually lead to a sharp decrease in the production of astaxanthin
[0005] According to the search, there are many patents on the production of astaxanthin by using Haematococcus pluvialis, but it has not been found to use lye to treat Haematococcus pluvialis after the green growth stage to improve harvesting efficiency, induction activity and resistance to diseases and insect pests report

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. Nutritional culture: BG-11 medium was used to expand Haematococcus pluvialis, the initial inoculation concentration was 0.05g / L, the temperature was 20~25℃, and the light was 70μmol / m 2 / s, the passage contains 1.5% CO 2 The mixed air was used for photoautotrophic culture for 10 days.

[0022] 2. Alkali pretreatment: Collect the above-mentioned cultured algae liquid into a stainless steel stirring tank, slowly add sodium hydroxide solution with a concentration of 10M to adjust the pH value of the algae liquid to 12.0, and control the temperature at 20-30℃. Stirring was continued for 0.5 hours under light conditions. The control group was not subjected to alkali pretreatment.

[0023] 3. Concentration of algal liquid: After alkali pretreatment, stop stirring, settle for 1 hour naturally, remove the supernatant medium, obtain concentrated algal liquid, and calculate the loss rate. The experimental data showed that the loss rate of the supernatant in the experimental...

Embodiment 2

[0027] 1. Nutritional culture: inoculate Haematococcus pluvialis into BG-11 medium, and place it in an outdoor photobioreactor for cultivation. Contains 1.0% CO 2 The mixed air was used for photoautotrophic culture for 10 days. Microscopic observation showed that bacteria, miscellaneous algae, protozoa and chytrid were present in the algal fluid.

[0028] 2. Alkali pretreatment: Collect the above-mentioned cultured algae liquid into a stainless steel stirring tank, slowly add sodium hydroxide solution with a concentration of 5M to adjust the pH value of the algae liquid to 11.0, and control the temperature at 20-30°C under no light conditions. Continue stirring for 2 hours. The control group was not subjected to alkali pretreatment.

[0029] 3. Concentration of algae liquid: After alkali pretreatment, stop stirring, settle naturally for 2 hours, remove the supernatant medium, obtain concentrated algae liquid, and calculate the loss rate. The experimental data showed that t...

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Abstract

The invention relates to the technical field of biology, and particularly provides a method for improving Haematococcus pluvialis to produce astaxanthin by using alkali pretreatment technology. The method includes the steps: firstly, adjusting PH (potential of hydrogen) value of the Haematococcus pluvialis subjected to nutrient culture to a range from 10.0 to 12.0 by adding high-concentration alkali liquor; removing supernate by standing and depositing after continuous stirring; inoculating the concentrate to an induction medium; inducing the astaxanthin under the conditions of high light, high temperature, high salinity and the like. The method is simple to perform; on one hand, Haematococcus pluvialis nutrient cell concentration time can be shortened, with loss rate reduced; on the other hand, the environmental tolerance and inducing activity of nutrient cells can be improved by the aid of a high-alkalinity environment; besides, invasive species in algal solution can be inhibited, the disease and pest damage resistance in the production process is improved, and the yield of astaxanthin is increased.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for improving the production of astaxanthin by Haematococcus pluvialis by utilizing an alkali pretreatment technology. Background technique [0002] Astaxanthin to combat adverse environmental conditions. [0003] According to the growth habit of Haematococcus pluvialis, the current mode of using Haematococcus pluvialis to produce astaxanthin generally adopts a two-step culture method, that is, the green growth stage and the astaxanthin induction stage of Haematococcus pluvialis under different conditions. realized below. In the first step, the vegetative reproduction of algal cells is realized by using a suitable culture environment, and the concentrated green algal cells are obtained after harvesting; the second step, the harvested green algal cells are inoculated into a new reactor, and the unfavorable conditions are used to realize shrimp production. The cy...

Claims

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Application Information

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IPC IPC(8): C12P23/00
CPCC12P23/00
Inventor 代云法赵坤郑玉瑞王华郑玉彬
Owner BIOALGO CY CO LTD
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