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Water culture method of arabidopsis thaliana

A technology of Arabidopsis thaliana and hydroponics, which is applied in the field of hydroponics of Arabidopsis thaliana, can solve the problems of affecting the growth of Arabidopsis thaliana seedlings, affecting the progress of experiments, and having many bacteria in the culture medium, so as to inhibit the growth of bacteria and eliminate impurities. Bacterial contamination, good growth effect

Inactive Publication Date: 2017-03-01
泉州齐美电子科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as the cultivation time is extended or the cultivation room is used for a long time, there will often be more bacteria in the culture solution and serious pollution, which will seriously affect the growth of Arabidopsis seedlings and seriously affect the progress of the experiment.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] An Arabidopsis hydroponic method, including the following steps:

[0040] Step 1. Soak the Arabidopsis seeds in an alcohol solution with a mass volume concentration of 60% for 10 minutes, then treat them for 3 days at a temperature of 0°C and dark conditions, and then place them in a solid medium to grow into a root length of 1.2 cm or more. Seedlings; the solid medium is 1 / 2MS solid medium.

[0041] Step 2. Move the seedlings with a root length of 1.2 cm or more obtained in Step 1 to a liquid culture medium for cultivation, and lay a layer of the first mixture layer of vermiculite and shell calcium carbonate on the bottom of the liquid medium, and set it at a temperature of 18°C The seedlings are obtained by culturing for 10 days under the conditions of 60% humidity, 2000-3000lux light and 24h light; the thickness of the first mixture layer is 1 / 5 of the depth of the liquid culture solution. The mass ratio of the vermiculite and the shell calcium carbonate in the first mix...

Embodiment 2

[0046] An Arabidopsis hydroponic method, including the following steps:

[0047] Step 1. Soak the Arabidopsis seeds in an alcohol solution with a mass volume concentration of 90% for 30 minutes, and then treat them for 4 days at a temperature of 4°C and dark conditions, and then place them in a solid medium to grow into a root length of 1.2 cm or more. Seedlings; the solid medium is 1 / 2MS solid medium.

[0048] Step 2. Move the seedlings with a root length of 1.2 cm or more obtained in step 1 to a liquid culture solution for cultivation, and lay a layer of the first mixture layer of vermiculite and shell calcium carbonate on the bottom of the liquid culture solution at a temperature of 22°C The seedlings are obtained by culturing for 15 days under the conditions of 80% humidity, 3000lux light and 24h light; the thickness of the first mixture layer is 1 / 3 of the depth of the liquid culture solution. The mass ratio of the vermiculite and the shell calcium carbonate in the first mixt...

Embodiment 3

[0053] An Arabidopsis hydroponic method, including the following steps:

[0054] Step 1. Soak the Arabidopsis thaliana seeds in an alcohol solution with 75% mass and volume concentration for 20 minutes, then treat them at a temperature of 24°C and dark conditions for 3 days, and then place them in a solid medium to grow into a root length of 1.2 cm or more. Seedlings; the solid medium is 1 / 2MS solid medium.

[0055] Step 2. Move the seedlings with a root length of 1.2 cm or more obtained in step 1 to a liquid culture medium for cultivation, and lay a layer of the first mixture layer of vermiculite and shell calcium carbonate on the bottom of the liquid medium, and set it at a temperature of 20°C The seedlings were obtained by culturing for 12.5 days under the conditions of humidity of 70%, light of 2500 lux and light of 24 hours; the thickness of the first mixture layer was 1 / 4 of the depth of the liquid culture solution. The mass ratio of the vermiculite and the shell calcium car...

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PUM

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Abstract

The invention discloses a water culture method of arabidopsis thaliana. The method comprises the steps that first, arabidopsis thaliana seeds are put in an alcoholic solution with the mass volume concentration of 60%-90% for soaking, then treated at the temperature of 0-4 DEG C in darkness and then put in a solid medium to be cultivated into seedlings with the root length being 1.2 cm or above; second, the seedlings are moved into a liquid culture solution for culture, a first mixture layer of vermiculite and shell calcium carbonate is laid at the bottom layer of the liquid culture solution, and culture is carried out for 10-15 days at the temperature of 18-22 DEG C, the humidity of 60%-80% and 24-h illumination of 2,000-3,000 lux to obtain plantlets; third, the liquid culture solution is changed once, meanwhile, a second mixture layer of vermiculite and eggshell powder is laid at the bottom layer of the new liquid culture solution, and the plantlets continue to be cultured for 10-15 days at the temperature of 18-22 DEG C, the humidity of 60%-80% and 24-h illumination of 2,000-3,000 lux; fourth, the second step and the third step are repeated cyclically till the arabidopsis thaliana plantlets are cultured to be in the period as demanded.

Description

Technical field [0001] The invention relates to a hydroponic culture method of Arabidopsis. Background technique [0002] Arabidopsis belongs to the phylum Angiosperm, Dicotyledon. The biennial herb is found in Mongolia, Xinjiang, Shaanxi, Gansu, Tibet, Shandong, Jiangsu, Anhui, Hubei, Sichuan, Yunnan and other provinces in China. The advantages of Arabidopsis are small plants and many knots. The genome of Arabidopsis is the smallest known plant genome. Arabidopsis thaliana is a self-pollinated plant with highly homozygous genes. The mutation rate is high when treated with physical and chemical factors, and it is easy to obtain defective types of various metabolic functions. Because of these advantages, Arabidopsis thaliana is a good material for genetic research and is hailed by scientists as the "fruit fly in plants". In scientific research, it is often necessary to use Arabidopsis seedlings or seedlings of different periods. Due to the rapid, convenient, rapid growth of se...

Claims

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Application Information

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IPC IPC(8): A01G31/00A01C1/08
CPCA01G31/00A01C1/08
Inventor 黄福萍
Owner 泉州齐美电子科技有限公司