A kind of medicine, composition and preparation thereof for preventing or treating diabetes
A technology of diabetes and medicine, applied in the field of compositions and preparations, medicines for preventing or treating diabetes
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Embodiment 1
[0027] Embodiment 1: the hypoglycemic effect of teruciside
[0028] experimental method
[0029] In order to study the effect of espressoside on type II diabetes, the classic type II diabetes model was used in this experiment, and C57BL mice were randomly divided into 3 groups, normal control group (10 mice), model group (10 mice), special female The quincetin treatment group (10 animals), in which the model group and the espressoside treatment group were given a high-fat diet (basic feed with 20% lard) to establish the model for 8 weeks, and the tequitoside treatment group was given a high-fat diet and oral administration at the same time. The rats were given by gavage (50 mg / kg / d), fasted overnight after the administration, and measured fasting blood glucose, glucose tolerance and insulin tolerance.
[0030] Glucose tolerance test method: mice were fasted overnight, and after oral administration of 2 g / kg glucose, blood was collected from the tail tip to measure the corre...
Embodiment 2
[0054] Example 2: The synergistic hypoglycemic effect of privetin and glimepiride
[0055] Disorders of sugar intake are one of the main causes of diabetes. The inventors starved the hepatocytes for 6 hours, and then gave corresponding drug treatments, taking the uptake of glucose by hepatocytes as an indicator of drug efficacy. Glucose uptake was measured using the glucose uptake assay kit from Ai Meijie Technology Co., Ltd., and the measurement method was carried out according to the instructions. Based on the Median-effect Principle, the CombiDrug Statistical Combination Index (CI) is used to quantitatively evaluate the synergistic, antagonistic or additive relationship between the two drugs.
[0056] The specific steps are as follows: the drug effect (fa) is the glucose uptake rate, according to the middle-effect equation fa / fu=(D / Dm) m , take the logarithm logfa / fu=mlogD-mlogDm on both sides, set a=-mlogDm, b=m, x=logD, y=logfa / fu, and substitute it into the middle-ef...
Embodiment 3
[0060] Embodiment 3: formulation example
[0061] quintessence tablet
[0062] Special privet glycosides 10mg, starch 88g, magnesium stearate 3g.
[0063] Preparation process: take terugenin, add starch and magnesium stearate, mix evenly, make into granules, dry, and press into tablets.
[0064] Special privet glycosides capsules
[0065] Special privet glycosides 10mg, starch 88g, magnesium stearate 3g.
[0066] Preparation process: take quincetin, add starch, and magnesium stearate, mix evenly, make into granules, dry, and pack into capsules.
[0067] quinquefasin injection
[0068] Special privet glycosides 47mg, sodium chloride injection 7mg.
[0069] Preparation process: take teruzheng glycosides and add 1.9mL water for injection to dissolve, add sodium chloride for injection to isotonic, adjust the pH value to 7-7.1, filter, refrigerate for 24 hours, add water for injection to a specified amount, filter, Potting, sterilization, and that's it.
[0070] Tetraprive...
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