Preparation method of chitosan oligosaccharide based polyelectrolyte sponge haemostatic dressing

A technology of polyelectrolyte and hemostatic dressing, applied in the fields of medical science, bandages, absorbent pads, etc., can solve the problems such as the inability of hemostatic methods and hemostatic drugs to meet the requirements, and achieve good biocompatibility and biodegradability, rapid absorption, Ease of Industrialization

Inactive Publication Date: 2017-03-08
INST OF METAL RESEARCH - CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] The purpose of the present invention is to provide a preparation method of polyelectrolyte sponge hemostatic dressing based on chitosan oligosaccharide, the polyelectrolyte sponge hemostatic dressing obtained shows excellent hemostatic function, and solves the existing conventional hemostatic methods and hemostatic drugs that cannot meet the requirements and other problems

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  • Preparation method of chitosan oligosaccharide based polyelectrolyte sponge haemostatic dressing

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preparation example Construction

[0027] In a specific embodiment, the preparation method of polyelectrolyte sponge hemostatic dressing based on chitosan oligosaccharide of the present invention, at first appropriate amount chitosan oligosaccharide and biocompatible anionic polyelectrolyte or amphoteric polyelectrolyte are dissolved in deionized water, then inject in the mold. By reducing the pH value of the polyelectrolyte mixture aqueous solution, the amino groups of chitosan molecules are protonated and positively charged, and interact with anionic polyelectrolytes or amphoteric polyelectrolytes to form complexes through Coulomb force and gradually gel. The polyelectrolyte complex gel was freeze-dried to obtain a spongy hemostatic dressing. The specific steps are:

[0028] 1) Prepare an aqueous solution of polyelectrolyte mixture containing chitosan oligosaccharide and anionic polyelectrolyte or amphoteric polyelectrolyte. Anionic polyelectrolytes or amphoteric polyelectrolytes are biocompatible polyelect...

Embodiment 1

[0034] 10 grams of chitosan oligosaccharide, 13 grams of sodium polyacrylate and 1 g of gluconolactone were dissolved in 1 liter of deionized water, and then poured into a 10 cm × 100 cm × 30 cm rectangular glass mold. After incubation at 50°C for 10 hours, the solution formed a gel. Gels were frozen at -18°C for 24 hours. After vacuum-drying for 60 hours under a vacuum degree of 5 Pa in a vacuum drying oven, a spongy chitosan polyelectrolyte hemostatic dressing was obtained. Such as figure 1 As shown in the photo of the chitosan polyelectrolyte sponge hemostatic dressing, the hemostatic dressing of this embodiment has good biocompatibility and bacteriostasis, exhibits excellent hemostatic function, and is easy to remove from the wound to accelerate wound recovery. The measurement of coagulation index in vitro in this embodiment shows that the coagulation index in vitro is <50%; the hemostatic effect test result of the rabbit ear artery bleeding model shows that the hemostat...

Embodiment 2

[0036] 10 grams of chitosan oligosaccharide, 10 grams of hyaluronic acid and 1 g of gluconolactone were dissolved in 1 liter of deionized water, and then poured into a 10 cm × 100 cm × 30 cm rectangular glass mold. After incubation at 50°C for 10 hours, the solution formed a gel. Gels were frozen at -18°C for 24 hours. After vacuum-drying for 60 hours under a vacuum degree of 5 Pa in a vacuum drying oven, a spongy chitosan polyelectrolyte hemostatic dressing was obtained. The hemostatic dressing of this embodiment has good biocompatibility and bacteriostasis, exhibits excellent hemostatic function, is easy to remove from the wound, and accelerates wound recovery. The measurement of coagulation index in vitro in this example shows that the coagulation index in vitro is <35%; the hemostatic effect test result of the rabbit ear artery bleeding model shows that the hemostatic time is <78 seconds.

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Abstract

The invention belongs to the field of biomedical material preparation, and relates to a preparation method of a chitosan oligosaccharide based polyelectrolyte sponge haemostatic dressing. At first, a proper amount of chitosan oligosaccharide and biocompatible anionic polyelectrolyte / amphoteric polyelectrolyte are dissolved into deionized water and then the mixture is injected into a mould. By reducing the pH value of the water solution of polyelectrolyte mixture, the amino group of the chitosan oligosaccharide molecules is protonated and carries positive charges. The chitosan oligosaccharide with positive charges interacts with anionic polyelectrolyte / amphoteric polyelectrolyte through the Coulomb force, compounds are formed, and gelation happens gradually. The compound gel is freeze-dried to obtain the sponge liked haemostatic dressing. The obtained haemostatic dressing has the advantages of good biocompatibility, good bacteriostatic activity, and excellent haemostatic performance, can be easily removed from wounds, and accelerates the healing speed of wounds. At the same time, the preparation technology is simple, and can be easily applied to industry.

Description

technical field [0001] The invention belongs to the field of preparation of biomedical materials, and relates to a preparation method of polyelectrolyte sponge hemostatic dressing based on chitosan oligosaccharide. Background technique [0002] Modern medical theory proves that wound healing is a continuous dynamic process of cell-to-cell, cell-to-cell matrix, and soluble media interactions. The wound repair process is related to the interaction of local tissue nutrition, microenvironment, growth factor content, microcirculation, hypoxic conditions, infection and other factors, and medical dressings can play a key role. As a main theoretical basis of high-tech medical dressings, the "wet therapy" discovered by British scientists in 1962 believes that when the surface of the wound is maintained in a moist microenvironment, the migration of epithelial cells is significantly accelerated, and the healing speed of the wound is faster than that of wounds. Fast in dry conditions. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/44A61L15/42A61L15/28
Inventor 赵岩张文昌张劲松
Owner INST OF METAL RESEARCH - CHINESE ACAD OF SCI
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