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Preparation method of difunctional nanometer enzyme for visual glucose detection

A nano-enzyme and dual-function technology, applied in the chemical field, can solve the problems of low activity, difficult separation, and easy oxidation of biological enzymes, and achieve the effects of high catalytic activity, good selectivity, and simple operation

Inactive Publication Date: 2017-03-08
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at technical defects such as poor accuracy of existing detection methods, easy oxidation of biological enzymes, low activity, difficult separation, and narrow applicability, the present invention discloses a synthetic rGO-ZnFe with peroxidase-like properties. 2 o 4 A New Approach to Nanozymes

Method used

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  • Preparation method of difunctional nanometer enzyme for visual glucose detection
  • Preparation method of difunctional nanometer enzyme for visual glucose detection
  • Preparation method of difunctional nanometer enzyme for visual glucose detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] (1) rGO-ZnFe 2 o 4 Synthesis of nanozymes

[0036] A. First weigh 1.0g of graphite powder in a mortar, add 50g of NaCl solid, grind for 10 minutes, add water to dissolve, filter, wash, and then remove NaCl. Transfer the treated graphite powder to a round bottom flask, add 23 mL concentrated H 2 SO 4 , stirred at room temperature for 8 hours. Then place the round bottom flask in an ice-water bath, slowly add 3.0g KMnO 4 Solid, stirred at 35-40°C for 30 minutes, then stirred at 65-80°C for 45 minutes. Then 46 mL of water was added and stirring was continued for 30 minutes at 98-105°C. Finally, add 140 mL of water and 10 mL of 30% H 2 o 2 The reaction was terminated, and the color of the solution turned bright yellow. The obtained product was washed with 5% HCl, then washed with ultrapure water, centrifuged (12000 rpm), and this process was repeated until it could not be separated by centrifugation. Then the obtained yellow suspension was transferred to a dialysi...

Embodiment 2

[0039] A. First weigh 1.0g of graphite powder in a mortar, add 50g of NaCl solid, grind for 10 minutes, add water to dissolve, filter, wash, and then remove NaCl. Transfer the treated graphite powder to a round bottom flask, add 23 mL concentrated H 2 SO 4 , stirred at room temperature for 8 hours. Then place the round bottom flask in an ice-water bath, slowly add 3.0g KMnO 4 Solid, stirred at 35-40°C for 30 minutes, then stirred at 65-80°C for 45 minutes. Then 46 mL of water was added and stirring was continued for 30 minutes at 98-105°C. Finally, add 140 mL of water and 10 mL of 30% H 2 o 2 The reaction was terminated, and the color of the solution turned bright yellow. The obtained product was washed with 5% HCl, then washed with ultrapure water, centrifuged (12000 rpm), and this process was repeated until it could not be separated by centrifugation. Then the obtained yellow suspension was transferred to a dialysis bag, and dialyzed in ultrapure water for one week un...

Embodiment 3

[0042] A. First weigh 1.0g of graphite powder in a mortar, add 50g of NaCl solid, grind for 10 minutes, add water to dissolve, filter, wash, and then remove NaCl. Transfer the treated graphite powder to a round bottom flask, add 23 mL concentrated H 2 SO 4 , stirred at room temperature for 8 hours. Then place the round bottom flask in an ice-water bath, slowly add 3.0g KMnO 4 Solid, stirred at 35-40°C for 30 minutes, then stirred at 65-80°C for 45 minutes. Then 46 mL of water was added and stirring was continued for 30 minutes at 98-105°C. Finally, add 140 mL of water and 10 mL of 30% H 2 o 2 The reaction was terminated, and the color of the solution turned bright yellow. The obtained product was washed with 5% HCl, then washed with ultrapure water, centrifuged (12000 rpm), and this process was repeated until it could not be separated by centrifugation. Then the obtained yellow suspension was transferred to a dialysis bag, and dialyzed in ultrapure water for one week un...

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Abstract

The invention discloses a novel method for preparing an rGO-ZnFe2O4 nanometer enzyme with a peroxidase-like property. On the basis, the catalysis performance of the nanometer enzyme is used for building a novel visual method for detecting the glucose content in urine; under the best condition, a glucose linear range measured by the method is 0.5 to 50 mu M; a linear regression equation is A=0.010c(mu M)+0.19(R=0.9913); a detection limit is 0.16 mu M. By using the method, whether a person suffers from diabetes or not can be judged by people with naked eyes.

Description

technical field [0001] The invention belongs to the field of chemistry, in particular to a bifunctional rGO-ZnFe for visually detecting glucose 2 o 4 Preparation method of nanozyme. Background technique [0002] Diabetes is a common metabolic disease that seriously threatens human health. According to the World Health Organization statistics, as of now, more than 285 million people in the world suffer from diabetes. It is estimated that by 2030 this number will be double or more than today. Diabetic patients generally show high blood sugar and positive urine sugar. Therefore, monitoring and controlling the blood sugar level in the human body is very important for diabetic patients. In recent years, researchers have been working hard to establish methods to detect glucose levels associated with diabetes. At present, people have established a variety of methods for measuring glucose content. Among them, the colorimetric method has attracted special attention of researche...

Claims

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Application Information

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IPC IPC(8): C12N9/08C12Q1/54C12Q1/28C12Q1/26
CPCC12N9/0065C12Q1/26C12Q1/28C12Q1/54C12Y111/01
Inventor 陈兴国马艳华
Owner LANZHOU UNIVERSITY
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