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Pseudomonas denitrificans and microbial inoculum thereof, and application thereof in degradation of quinclorac

A technology of Pseudomonas denitrification and quinclorac, which is applied in the direction of bacteria, chemical instruments and methods, and methods based on microorganisms, can solve the problems of long residual period, pollution, soil damage, etc., and achieve fast growth, Simple operation, powerful effect

Active Publication Date: 2017-03-15
粮华生物科技(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Quinclorac (Quinclorac) is a hormone-type quinolinic acid paddy field herbicide that was promoted by BASF in the 1980s. It has a good weeding effect especially for controlling barnyardgrass in rice fields, but it has a long residual period.
Therefore, quinclorac-based pesticide residues in the soil have adverse effects on the growth and quality of tobacco, and will bring serious economic losses to tobacco leaf production.
[0004] At present, the traditional treatment methods for pesticide residues in soil mainly include physical and chemical methods such as soil replacement, leaching, chemical passivation, electrical repair, thermal repair, etc., but generally speaking, these technologies have certain limitations. The cost of implementation is high, and the damage to the soil is serious, and it is easy to cause other pollution

Method used

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  • Pseudomonas denitrificans and microbial inoculum thereof, and application thereof in degradation of quinclorac
  • Pseudomonas denitrificans and microbial inoculum thereof, and application thereof in degradation of quinclorac
  • Pseudomonas denitrificans and microbial inoculum thereof, and application thereof in degradation of quinclorac

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] This example is used to illustrate the tolerance and degradability of Pseudomonas denitrificans provided by the present invention to Quinclorac in the aqueous phase (LB liquid medium)

[0052] The strain W-7 was inserted into the sterilized LB liquid culture with the concentration of 30mg / L, 50mg / L, 100mg / L, 200mg / L and 300mg / L quinclorac at 1% by volume inoculum. 基中. Cultivated at 170rpm and 30±1℃ for 24h, the results showed that the strain W-7 showed good tolerance to Quinclorac, and the strain could grow in different media. The maximum OD value that the bacteria can reach and the degradation rate of Quinclorac are shown in Table 1.

Embodiment 2

[0060] This example is used to illustrate the tolerance and degradability of Pseudomonas denitrificans provided by the present invention to Quinclorac in the aqueous phase (inorganic salt medium)

[0061] The strain W-7 was cultured with an inoculum volume of 1% by volume and sterilized with an inorganic salt of quinclorac with a concentration of 30mg / L, 50mg / L, 100mg / L, 200mg / L and 300mg / L. 基中. Incubate at 170rpm and 30±1℃ for 24h. The results showed that the strain W-7 showed good tolerance to Quinclorac, and the strain could grow in different media. With Quinclorac as the sole carbon source, the maximum OD value that the bacteria can reach and the degradation rate of Quinclorac are shown in Table 1.

Embodiment 3

[0072] This example is used to illustrate the degradability of Quinclorac in the soil of Pseudomonas denitrificans provided by the present invention

[0073] Strain W-7 was inoculated into 100 mL of LB liquid medium at 1 vol% strain, 175rmp, 30±1°C for 24h, then the bacterial liquid was added to 5kg containing 20mg / kg quinclorac In the soil, stir evenly and cultivate for 7 days to ensure that the water content in the soil is about 20%. The content of quinclorac in the soil was measured on the 3rd, 5th and 7th days. The results are shown in Table 2.

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Abstract

The invention discloses pseudomonas denitrificans having the preservation number of CGMCC No.10845. The invention also discloses a microbial inoculum comprising the pseudomonas denitrificans. The invention further discloses an application of the pseudomonas denitrificans and the microbial inoculum in degradation of quinclorac. The invention also discloses a method for degradation of quinclorac. The pseudomonas denitrificans is characterized in that: (1) only an inorganic salt culture medium is provided, and the pseudomonas denitrificans can survive and grow in a culture environment containing quinclorac as a sole carbon source, and has the advantages of strong vital force, fast growth speed, and large living weight; (2) during preparation, only a preservation strain needs to be activated, the operation is simple and the cost is low; and (3) the pseudomonas denitrificans has high degradation effect in water bodies and soil, in the water bodies, under a condition that the content of quinclorac is 200 mg / L, the bacteria are cultured for 24 h, the degradation rate is as high as 93.4%, and in the soil, under a condition that the content of quinclorac is 20 mg / kg, the degradation rate is as high as 88.0%.

Description

Technical field [0001] The present invention relates to the field of microbial environmental restoration, in particular, to a strain of Pseudomonas denitrificans with quinclorac degrading function, a bacterial agent containing the Pseudomonas denitrificans, and their degradation Quinclorac's application. Background technique [0002] Quinclorac is a hormone-based quinclorac paddy field herbicide promoted by BASF in Germany in the 1980s. It has a good weeding effect especially for the control of barnyardgrass in rice fields, but it has a longer residual period. Its chemical name is 3,7-dichloroquinoline-8-carboxylic acid and its molecular formula is C 10 H 5 C l2 NO 2 , The molecular weight is 942.1Da, and the product names are Keyixing, Kuaishayi, Shenshu, Yicaojing, Shayiling, and Barnyard Grass. Its target of action is the synthetic hormones in plants. By interfering with plant hormones to regulate enzyme activity, the growth and metabolism of organisms can not proceed normall...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/38C02F101/38
Inventor 任立伟齐丹林森王培红
Owner 粮华生物科技(北京)有限公司
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