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Method for detecting ToRCH10 item antibody based on filter paper dried blood spots

A dry blood spot and antibody technology, applied in the field of antibody detection, can solve the problems of large amount of samples, difficult storage or transportation of samples, etc., and achieve the effect of reducing sample size, convenient storage and transportation, and less damage to the human body

Inactive Publication Date: 2017-03-15
HANGZHOU QUANTUM HEALTH TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Based on this, it is necessary to provide a method for detecting ToRCH10 antibodies based on dry blood spots on filter paper in view of the large amount of samples used in the detection of ToRCH10 antibodies and the problems that the samples are not easy to store or transport

Method used

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  • Method for detecting ToRCH10 item antibody based on filter paper dried blood spots
  • Method for detecting ToRCH10 item antibody based on filter paper dried blood spots
  • Method for detecting ToRCH10 item antibody based on filter paper dried blood spots

Examples

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Effect test

Embodiment 1

[0070] Take Toxoplasma gondii, rubella virus, cytomegalovirus, and herpes simplex virus type 1 / type 2 IgG antibody detection kit, each of 10 μL of control substance, negative quality control substance, positive quality control substance 1-2, and venous serum C1-C6, Dilute with 200 μL of IgG sample diluent in Toxoplasma gondii, rubella virus, cytomegalovirus, and herpes simplex virus type 1 / 2 IgG antibody detection kits respectively to prepare reference dilution, negative quality control dilution, and positive quality control dilution. Control product 1-2 dilution, venous serum C1-C6 dilution;

[0071] Punch four discs with a diameter of 3 mm on the dried blood spots S1-S6 of the filter paper with a hole puncher and put them into a centrifuge tube. The IgG sample diluent in the cell virus and herpes simplex virus type 1 / 2 IgG antibody detection kit was reconstituted by shaking at room temperature for 1 hour to obtain reconstituted samples S1-S6.

[0072] step 1

[0073] Add 5...

Embodiment 2

[0086] Take 10 μL each of the control substance, negative quality control substance, positive quality control substance, and venous serum C1-C6 in the detection kit for Toxoplasma gondii, rubella virus, cytomegalovirus, and herpes simplex virus type 1 / 2 IgM antibody, and use 200 μL each Toxoplasma gondii, rubella virus, cytomegalovirus, and herpes simplex virus type 1 / type 2 IgG antibody detection kits were diluted with the IgM sample diluent to prepare reference substance dilution, negative quality control dilution, and positive quality control dilution solution, venous serum C1-C6 dilution;

[0087] Punch four discs with a diameter of 3 mm on the dried blood spots S1-S6 of the filter paper with a hole puncher and put them into a centrifuge tube. The ToRCH IgM sample diluent in the cell virus and herpes simplex virus type 1 / 2 IgM antibody detection kit was reconstituted by shaking at room temperature for 1 hour to obtain reconstituted samples S1-S6.

[0088] step 1

[0089]...

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Abstract

The invention provides a method for detecting a ToRCH10 item antibody based on filter paper dried blood spots. The method includes the following steps that blood slices with the diameter of 2-4 mm are obtained from the filter paper sheet dried blood spots; the blood slices are re-dissolved with sample diluent, and re-dissolved samples are prepared; the re-dissolved samples are measured through a streaming fluorescence radiation method. According to the method for detecting the ToRCH10 item antibody based on the filter paper dried blood spots, an IgG antibody or an IgM antibody is measured through the combination of a dried whole blood filter paper method and the streaming fluorescence radiation method, the problem that the needed sample size is large in an existing detection method is solved, the needed sample size is reduced, harm to the human body is small, the samples are convenient to save and transport, especially, when objects are newborns, no venous blood sampling is needed, and sampling is more convenient.

Description

technical field [0001] The invention relates to the field of antibody detection methods, in particular to a method for detecting ToRCH10 antibodies based on filter paper dried blood spots. Background technique [0002] The term ToRCH was proposed by the American scholar Nahmias in 1971. It consists of the initials of the English names of various microorganisms and viruses that cause intrauterine infections. Among them, To represents Toxop Lasmagondii (Tox), R represents rubella virus (RubeLLaVirus, Rub), C represents cytomegalovirus (CytomegaLoVirus, CMV), H represents herpes simplex virus type 1 and type 2 (Herpes SimpLexVirus 1 and 2, HSV1 / 2). ToRCH infection is an important risk factor for intrauterine infection. After a pregnant woman is infected with one or more of these pathogens, it can be transmitted to the fetus through the placenta or cause perinatal infection through the birth canal, resulting in miscarriage, stillbirth, premature delivery, congenital deformity a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/569G01N33/543G01N33/533
CPCG01N33/6854G01N33/533G01N33/54326G01N33/56905G01N33/56983G01N33/56994G01N2469/20
Inventor 陈姗姗骆亦奇余国良
Owner HANGZHOU QUANTUM HEALTH TECH CO LTD
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