Method for degrading residual polyacrylamide in slime water through composite bacteria

A technology of polyacrylamide and compound strains, which is applied in the direction of microorganism-based methods, chemical instruments and methods, water/sludge/sewage treatment, etc., and can solve the problems that polyacrylamide research has not been reported.

Inactive Publication Date: 2017-03-22
ANHUI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there is no report on the degradative treatment of residual polyacrylamide in coal slime water by using the biodegradation method of compound strains

Method used

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  • Method for degrading residual polyacrylamide in slime water through composite bacteria
  • Method for degrading residual polyacrylamide in slime water through composite bacteria

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0035] 1) Get the Erlenmeyer flask containing 100ml degradation medium, wherein the concentration of polyacrylamide is 100mg / L, with 2% Phanerochaete chrysosporium spore suspension and 2% Rhodopseudomonas sphaeroides suspension Added together to the degradation medium;

[0036] 2) Use a pen-type pH meter to adjust the pH value of the medium to 5, and place it in a shaking incubator at 30°C and 130r / min for continuous cultivation for 6 days;

[0037] 3) Measure the absorbance of the degradation medium at 660 nm with a UV-5100 ultraviolet spectrophotometer every day during the culture period, and compare with the standard to obtain the corresponding polymer concentration, and the degradation rate is 32.5%. Under other conditions unchanged, the polyacrylamide biodegradation bacteria were replaced with 2ml of Rhodopseudomonas sphaeroides suspension, the degradation rate was 21.9%, and the polyacrylamide biodegradation bacteria were replaced with 2ml of Chrysosporium Phaneroderma ...

example 2

[0039] 1) Get the Erlenmeyer flask containing 100ml degradation medium, wherein the concentration of polyacrylamide is 100mg / L, with 3% Phanerochaete chrysosporium spore suspension and 3% Rhodopseudomonas sphaeroides suspension Added together to the degradation medium;

[0040] 2) Use a pen-type pH meter to adjust the pH value of the medium to 6, and place it in a shaking incubator at 30°C and 140r / min for continuous cultivation for 6 days;

[0041] 3) During the culture period, the absorbance of the degradation medium was measured at 660nm with a UV-5100 ultraviolet spectrophotometer every day, and the corresponding polymer concentration could be obtained by comparing with the standard, and the degradation rate was 34.7%. Under other conditions unchanged, the polyacrylamide biodegradation bacteria were replaced with 3ml of Rhodopseudomonas globosa suspension, the degradation rate was 23.2%, and the polyacrylamide biodegradation bacteria were replaced with 3ml of Chrysosporium...

example 3

[0043] 1) Get the Erlenmeyer flask containing 100ml degradation medium, wherein the concentration of polyacrylamide is 100mg / L, with 4% Phanerochaete chrysosporium spore suspension and 4% Rhodopseudomonas sphaeroides suspension Added together to the degradation medium;

[0044] 2) Use a pen-type pH meter to adjust the pH value of the medium to 7, and place it in a shaking incubator at 30°C and 150r / min for continuous cultivation for 6 days;

[0045] 3) Measure the absorbance of the degradation medium at 660 nm with a UV-5100 ultraviolet spectrophotometer every day during the culture period, compare with the standard to obtain the corresponding polymer concentration, and the degradation rate is 33.4%. Under other conditions unchanged, the polyacrylamide biodegradation bacteria were replaced with 4ml of Rhodopseudomonas globosa suspension, and the degradation rate was 22.9%. Phaneroderma spore suspension, the degradation rate was 31.4%.

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Abstract

The invention relates to a method for degrading residual polyacrylamide in slime water through composite bacteria. According to the method, rhodopseudomonas spheroid bacterial suspension and phanerochaete chrysosporium spore suspension are used as composite degrading bacterial suspension, the rhodopseudomonas spheroid bacterial suspension and the phanerochaete chrysosporium spore suspension are added into a degrading culture medium with the volume ratio being 1 to X under certain concentration of polyacrylamide, and under the condition that external conditions are adjusted, specifically, PH of the degrading culture medium comprising the polyacrylamide is adjusted to 5-7, and the culture temperature is adjusted to 25-50 DEG C, the polyacrylamide is degraded through a series of biodegradation functions. The method has the advantages that the polyacrylamide degradation effect is good, defects of a single bacterium can be overcome, the synergistic effect of different bacteria is fully achieved, the adverse effects of all factors on the degradation effect are reduced, and the degradation adaptability is higher.

Description

technical field [0001] The invention relates to a method for degrading residual polyacrylamide in coal slime water by composite bacteria Background technique [0002] Coal slime water is a kind of coal preparation wastewater produced during the coal washing process. At present, the slime water treatment mainly adopts the addition of organic polymer flocculants and inorganic flocculants to accelerate the flocculation and settlement of slime particles. Polyacrylamide in organic polymer flocculants is widely used to improve the flocculation rate of coal slime water because of its long molecular chain and a large number of active groups on the side chain. However, the large-scale use of polyacrylamide will also bring some negative effects: on the one hand, residual polyacrylamide accumulates in the circulating water of the coal preparation plant, and some polyacrylamide is adsorbed on the coal slime particles before adding flotation agents, resulting in The adsorption capacity...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C12N1/20C12N1/14C12R1/38C12R1/645C02F101/38
CPCC02F3/34C02F2101/38C12N1/14C12N1/20
Inventor 张东晨戴雯闫佳冀敏敏范晓露常飞
Owner ANHUI UNIV OF SCI & TECH
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