A kind of EGFR immunohistochemical detection kit based on Fe3O4 nanoprobe and its application method

A detection kit and nanoprobe technology are applied in the field of biological nanomaterials and their preparation to achieve the effects of simple operation, small size and high peroxidase-like activity
CN106525827BActive Publication Date: 2019-04-09NANJING NANOEAST BIOTECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
NANJING NANOEAST BIOTECH
Publication Date
2019-04-09

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Abstract

An EGFR immunohistochemical detection kit based on Fe<3>O4 nanoprobes and a using method thereof are disclosed. The kit includes a citric acid restoring powder agent, an endogenous peroxidase blocker, a non-specific binding site blocker, a DAB concentrate liquid, a DAB chromogenic substrate, a DAB diluting liquid, the Fe<3>O4 nanoprobes, a TBS powder agent and a hematoxylin dyeing liquor. A novel method of bonding nanometer particles and an antibody is provided to skillfully combine high peroxidase-like activity of the Fe<3>O4 nanometer particles and biological characteristics of an EGFR single-domain antibody, and a high amount of the EGFR single-domain antibody is loaded on the surfaces of the prepared Fe<3>O4 nanoprobes. The Fe<3>O4 nanoprobes are formed by the Fe<3>O4 nanometer particles surfaces of which are loaded with metal ions and the EGFR single-domain antibody the C end of which is provided with His-Tags, and has advantages of a small size, good stability, high specificity, high sensitivity and high catalytic activity. The EGFR expression level of a tumor pathological tissue can be rapidly and conveniently detected through the kit, thus providing bases for tumor treatment.
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Description

technical field

[0001] The invention relates to a bionano material and a preparation method thereof, in particular to a Fe3O4 nanometer probe for detecting EGFR and a preparation method thereof. Background technique

[0002] Immunohistochemical detection, referred to as immunohistochemical detection, is a specific antibody labeled with a chromogenic agent in situ through antigen-antibody reaction and marker display reaction in tissue cells to locate, characterize, and Bioassay techniques for quantitative determination. Immunohistochemistry is a pathological diagnostic method for tumor diagnosis and differentiation, which is widely used in clinic. Its application directions include: (1) Diagnosis and identification of malignant tumors; (2) Pathological typing of certain types of tumors; (3) Determining the primary site of metastatic malignant tumors; (4) Diagnosis of soft tissue tumors; (5) Identify and discover micrometastases, and provide reference for the formulation of ...

Claims

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