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Method for detecting maleic hydrazide in tobacco and tobacco products

A technology for tobacco products and maleic hydrazide, which is applied in the detection field of maleic hydrazide residues, can solve the problems of low accuracy, false positive detection, complicated pretreatment, etc., and achieve good resolution and peak shape

Active Publication Date: 2017-03-22
SICHUAN BRANCH OF CHINA TOBACCO
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Problems solved by technology

[0003] The analysis methods of maleic hydrazide residues in tobacco mainly include spectrophotometry (ISO4876-1980), high performance liquid chromatography (HPLC, YC / T405.5-2011), gas chromatography (GC) and micellar liquid chromatography. Chromatography (MLC), the method widely used in the tobacco industry to determine maleic hydrazide residues in tobacco is the HPLC-UV method (YC / T405.5-2011). This method uses a UV detector with low sensitivity. The pre-processing is very complicated, and the external standard method is selected for quantification. The accuracy of the method is relatively low, and false positive detection is prone to occur.

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  • Method for detecting maleic hydrazide in tobacco and tobacco products
  • Method for detecting maleic hydrazide in tobacco and tobacco products

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Embodiment Construction

[0037] The following will be described in conjunction with a specific embodiment.

[0038] 1. Reagents and instruments

[0039] Maleic hydrazide and maleic hydrazide-d 2 Standards were from Labor Dr. Ehrenstorfer-Schafers (chemical purity: ≥98.5%, Augsburg, Germany), formic acid (HPLC grade, Sigma Company, Germany); methanol, acetonitrile (chromatographically pure, Thermo-Fisher Company, USA), hydrochloric acid (analytical Pure, Xilong Science Co., Ltd.), N-propylethylenediamine (PSA) was purchased from Agilent (USA), and water was ultrapure water.

[0040] Ultra-high performance liquid chromatography-tandem mass spectrometer (Xevo TQ, Waters, USA), equipped with electrospray ionization source (ESI); vortex oscillator (VtexMixer 230VeU, Labnet, USA).

[0041] 2. Standard solution preparation

[0042] Accurately weigh 10 mg of maleic hydrazide standard substance, accurate to 0.0001g, dissolve it in ultrapure water to 10 mL, prepare 1.0 mg / mL standard stock solution, and stor...

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Abstract

The invention discloses a method for detecting maleic hydrazide in tobacco and tobacco products that include tobacco leaves and tobacco shreds. The method comprises the steps of preparing a standard solution, reflow-extracting with hydrochloric acid, carrying out disperse solid extraction and purification, and carrying put UPLC-MS / MS analytical determination. For the tobacco bud inhibitor maleic hydrazide, ultrahigh-performance liquid chromatography tandem mass spectrometry (UPLC-MS / MS) is used to establish the method for quickly analyzing maleic hydrazide residue in tobacco and tobacco products by UPLC-MS / MS. The tandem mass spectrometry selected herein is significantly higher than ultraviolet detectors of the standard method (YC / T 405.5-2011) in both separation degree and sensitivity.

Description

technical field [0001] The invention belongs to the field of pesticide residue detection and relates to a detection method for maleic hydrazide residues in tobacco and tobacco products. Background technique [0002] Maleic hydrazide, also known as Yayadan, is a plant growth regulator, which is commonly used in tobacco production to inhibit the growth of tobacco lateral buds, and is widely used abroad. Due to its long-term and repeated application during the tobacco field growth period, it will cause its residue in the first-cured tobacco leaves, and the residue of maleic hydrazide in the tobacco leaves will cause health risks to smokers. International Tobacco Research Cooperation Center (CORESTA) Include it in the 118th list of tobacco pesticide guidance residue limit list). [0003] The analysis methods of maleic hydrazide residues in tobacco mainly include spectrophotometry (ISO4876-1980), high performance liquid chromatography (HPLC, YC / T405.5-2011), gas chromatography (...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 熊巍陶晓秋庞夙韶济民张海燕杨雪
Owner SICHUAN BRANCH OF CHINA TOBACCO
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