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Method for producing clostridium butyricum by using pichia pastoris fermentation bacterial sludge

A technology of Clostridium butyricum and Pichia pastoris is applied in the field of fermentation engineering to achieve the effects of reducing production cost, improving utilization rate and reducing environmental protection pressure

Inactive Publication Date: 2017-03-29
湖北华扬科技发展有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method of using Pichia pastoris fermentation sludge to produce Clostridium butyricum, which can not only solve the problems existing in the treatment of Pichia fermentation sludge, but also replace the nitrogen required in the cultivation of Clostridium butyricum source, reduce the production cost of the enterprise

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  • Method for producing clostridium butyricum by using pichia pastoris fermentation bacterial sludge
  • Method for producing clostridium butyricum by using pichia pastoris fermentation bacterial sludge
  • Method for producing clostridium butyricum by using pichia pastoris fermentation bacterial sludge

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Embodiment 1

[0052] This embodiment provides a method for producing Clostridium butyricum using Pichia pastoris fermentation sludge, comprising the following steps:

[0053] 1) Dilute Pichia pastoris fermentation sludge and tap water at a mass ratio of 1:1 to prepare a yeast suspension.

[0054] 2) The yeast suspension is placed in a stirring reactor, and stirred at a low speed at 50° C. for 24 hours, and the stirring is limited to achieve uniform mixing.

[0055] 3) The yeast suspension treated in step 2) is subjected to spray drying and dehydration treatment, the air inlet temperature is 180° C., and the air outlet temperature is 70° C. to obtain yeast powder with a water content of 8.4%.

[0056] 4) Use a 7L glass fermenter to carry out the fermentation experiment of Clostridium butyricum, and the fermentation medium of Clostridium butyricum in the control tank is: glucose 2% (w / v), soybean meal 4.5% (w / v), ammonium sulfate 0.1% (w / v), NaHCO 3 0.124% (w / v), corn flour 0.7% (w / v), MnSO...

Embodiment 2

[0063] This embodiment provides a method for producing Clostridium butyricum using Pichia pastoris fermentation sludge, comprising the following steps:

[0064] 1) Dilute Pichia pastoris fermentation sludge and tap water at a mass ratio of 1:1 to prepare a yeast suspension.

[0065] 2) Put the yeast suspension in a stirred reactor, add 1% NaCl into the stirred reactor at the same time, stir at 50° C. for 24 hours at a low speed, and stir until uniform mixing is achieved.

[0066] 3) The yeast suspension treated in step 2) is subjected to spray drying and dehydration treatment, the air inlet temperature is 180° C., and the air outlet temperature is 70° C. to obtain yeast powder with a water content of 7.3%.

[0067] 4) A 30L stainless steel fermenter was used to carry out the fermentation experiment of Clostridium butyricum, and the fermentation medium of Clostridium butyricum in the control tank was: glucose 2% (w / v), soybean meal 4.5% (w / v), ammonium sulfate 0.1% (w / v), NaHC...

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Abstract

The invention provides a method for producing clostridium butyricum by using pichia pastoris fermentation bacterial sludge, wherein the method comprises the following steps: 1) diluting the pichia pastoris fermentation bacterial sludge into a yeast suspension; 2) placing the yeast suspension in a stirring reactor, and stirring and mixing evenly; 3) carrying out spray drying dehydration treatment of the evenly-mixed yeast suspension, to obtain a yeast powder; 4) adding the yeast powder instead of an organic nitrogen source to a clostridium butyricum culture medium, and carrying out anaerobic fermentation, to obtain a clostridium butyricum fermentation liquid; and 5) concentrating the clostridium butyricum fermentation liquid, and carrying out spray drying treatment, to obtain a clostridium butyricum powder preparation. The method can solve the problem of processing of the pichia pastoris fermentation bacterial sludge, reduces environmental pressure, achieves low emission of industrial production, also enables the nitrogen source required for clostridium butyricum culture to be replaced, reduces the production cost of enterprises, and has good market application prospects.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering, and in particular relates to a method for producing Clostridium butyricum by using Pichia pastoris fermentation sludge. Background technique [0002] With the large-scale industrialization of the recombinant protein technology of Pichia pastoris, a large amount of Pichia pastoris fermentation sludge is produced every year. The main components of these sludges include a large number of yeast cells, raffinate, medium and extracellular proteins. However, there are the following problems in the treatment of these microorganisms: (1) Pichia pastoris is basically a genetically modified variety, and it is not appropriate to adopt the method of landfilling or straight discharge under the premise of sufficient experimental data, and must be treated to a certain extent; (2) yeast The hydrolyzate is extremely nutritious. Once it enters the sewage system, it will greatly increase the COD val...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/145C12R1/84
CPCC12N1/20
Inventor 谌颉熊晓燕周樱詹志春
Owner 湖北华扬科技发展有限公司