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Method for rapidly detecting salmonella in textiles

A technology for salmonella and textiles, applied in the field of salmonella, can solve the problems of long cycle, shortened detection time, cumbersome procedures, etc.

Active Publication Date: 2017-03-29
INSPECTION & QUARANTINE TECH CENT HENAN ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional detection methods are cumbersome, long-term, and low-sensitivity. The development of molecular biology methods, immunological methods, and electrical impedance methods has greatly improved the detection sensitivity of Salmonella, shortened the detection time, and simplified the detection procedures. However, these methods can only be used as preliminary methods. Screening method, the final positive judgment result must return to the traditional standard method
The above detection methods are widely used in the food field, but there are few reports on the detection methods of Salmonella in the textile field

Method used

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  • Method for rapidly detecting salmonella in textiles
  • Method for rapidly detecting salmonella in textiles
  • Method for rapidly detecting salmonella in textiles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0092] A method for rapidly detecting salmonella in textiles, comprising the steps of:

[0093] Step 1: Sample enrichment

[0094] Aseptically open the textile sample for inspection, cut the sample evenly with sterile scissors, accurately weigh 25g of the cut sample on an electronic balance, cut it into pieces and add it to a sterile homogeneous bag containing 225mL of SCDLP liquid medium. Beat with a slap-type homogenizer for 1min to 2min, and mix thoroughly; put the prepared sample into a constant temperature incubator at 36°C±1°C, and enrich the bacteria for 16h±2h to obtain the first enrichment solution;

[0095] Step 2: LAMP test

[0096] 2.1) Preparation of DNA template: Take 1mL of SCDLP enrichment solution and put it into a 1.5mL sterile Eppendorf tube, centrifuge in a high-speed refrigerated centrifuge at 10,000r / min for 5min, discard the supernatant; add 1mL of sterilized double-distilled water to mix, and then centrifuge Centrifuge at 10,000r / min in the machine fo...

Embodiment 2

[0113] A method for rapidly detecting salmonella in textiles, comprising the steps of:

[0114] Step 1: Sample enrichment

[0115] Aseptically open the textile sample for inspection, cut the sample evenly with sterile scissors, accurately weigh 25g of the cut sample on an electronic balance, cut it into pieces and add it to a sterile homogeneous bag containing 225mL of SCDLP liquid medium. Beat with a slap-type homogenizer for 1min to 2min, and mix thoroughly; put the prepared sample into a constant temperature incubator at 36°C±1°C, and enrich the bacteria for 16h±2h to obtain the first enrichment solution;

[0116] Step 2: LAMP test

[0117] 2.1) Preparation of DNA template: Take 1mL of SCDLP enrichment solution and put it into a 1.5mL sterile Eppendorf tube, centrifuge at 10000r / min in a high-speed refrigerated centrifuge for 5min, discard the supernatant; add 1mL of sterilized double-distilled water, mix well, and centrifuge in a high-speed refrigerated centrifuge Centri...

Embodiment 3

[0134] A method for rapidly detecting salmonella in textiles, comprising the steps of:

[0135] Step 1: Sample enrichment

[0136] Open the textile sample submitted for inspection by aseptic method, cut the sample evenly with sterile scissors, accurately weigh 25g of the cut sample on the electronic balance, cut it into pieces and add it to a sterile homogeneous bag containing 225mL of SCDLP liquid medium. Beat with a slap-type homogenizer for 1 to 2 minutes, and mix thoroughly; put the prepared sample into a constant temperature incubator at 36°C±1°C, and enrich the bacteria for 16h±2h to obtain the first enrichment solution;

[0137] Step 2: LAMP test

[0138] 2.1) Preparation of DNA template: Take 1mL of SCDLP enrichment solution and put it into a 1.5mL sterile Eppendorf tube, centrifuge at 10000r / min in a high-speed refrigerated centrifuge for 5min, discard the supernatant; add 1mL of sterilized double-distilled water, mix well, and centrifuge in a high-speed refrigerated...

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Abstract

The invention discloses a method for rapidly detecting salmonella in textiles. The method comprises the following steps: I, enriching bacteria of a sample; II,conducting an LAMP test; II, conducting MALDI-TOF-MS technical identification; and IV, reporting a result. With the application of the method, a detection duration is greatly shortened, a negative detection result can be obtained within 1 day and a positive detection result can be obtained within 3 days. The method, through the scientific combination of the LAMP technology and the MALDI-TOF-MS, is simple and economic, and meanwhile, the method has the characteristic of being high in throughput; the method is applicable to instant and in-situ detection; and when the in-situ detection is positive, samples can be purposefully increased and inspected in laboratory, so that the method, when supervised, is high in reaction speed and strongly targeted, and moreover, the method is time-saving.

Description

technical field [0001] The invention relates to a method for salmonella, in particular to a method for rapidly detecting salmonella in textiles. Background technique [0002] The main background of Salmonella detection in textiles: Salmonella (Salmonella) is a kind of zoonotic pathogenic bacteria, a member of the Enterobacteriaceae Salmonella genus, and a class of conditional intracellular parasitic Gram-negative enterobacteria. The bacterium has low nutritional requirements and is widely distributed in nature. It can be detected in food, water, soil, animal hair, and fiber. It can infect many animals and humans, and most of them are highly pathogenic. More than 2,500 Salmonella serotypes have been discovered so far, many of which can cross-infect humans and animals, and can cause a variety of different clinical manifestations in humans and animals, mainly causing fever, gastroenteritis, diarrhea and sepsis etc., causing serious harm to human health. In recent years, with ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/10C12R1/42
CPCC12Q1/6844C12Q1/689C12Q2531/119C12Q2565/627
Inventor 李轲郭会清郭华麟张淑霞徐超乔晴禹建鹰张超峰
Owner INSPECTION & QUARANTINE TECH CENT HENAN ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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