A kind of aziridine type [iron-iron] hydrogenase active center model substance containing phosphine ligand and its synthesis method
A technology of hydrogenase activity and synthesis method, applied in the field of synthesis, can solve problems such as low catalytic hydrogen release performance, and achieve the effects of simple operation, high yield and mild conditions
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Embodiment 1
[0031]A method for synthesizing the model substance 1 of the active center of azapropylene [iron-iron] hydrogenase containing a phosphine ligand, the chemical formula of the model substance 1 is Fe 2 [(SCH 2 ) 2 NCH 2 CH 2 CH 2 SMe] (CO) 5 [P(C 6 h 5 ) 3 ], its chemical synthesis process is as follows:
[0032]
[0033] Concrete synthetic steps are as follows:
[0034] Under the protection condition of inert gas, add 344mg Fe in the flask equipped with stirring magnet 2 S 2 (CO) 6 (1mmol) and 15mL of tetrahydrofuran solvent to obtain a dark red solution, the resulting solution was cooled to -78°C with a liquid nitrogen bath, and 2mL of lithium triethylborohydride (1M in THF) solution was slowly added under stirring conditions and reacted for 15min Finally, add 0.16mL trifluoroacetic acid (2mmol), continue the reaction for 15min, add 0.17mL aqueous formaldehyde solution (37%), raise the reaction system to room temperature, stir the reaction for 1h, then add 288mg...
Embodiment 2
[0039] A method for synthesizing the model object 2 of the active center of azapropylene [iron-iron] hydrogenase containing a phosphine ligand, the chemical formula of the model object 2 being Fe 2 [(SCH 2 ) 2 NCH 2 CH 2 CH 2 SMe] (CO) 5 (Ph 2 PC 5 h 4 N), its chemical synthesis process is as follows:
[0040]
[0041] Under the protection condition of inert gas, add 344mg Fe in the flask equipped with stirring magnet 2 S 2 (CO) 6 (1mmol) and 15mL of tetrahydrofuran solvent to obtain a dark red solution, the resulting solution was cooled to -78°C with an acetone bath, and 2mL of lithium triethylborohydride (1M in THF) was slowly added under stirring conditions. After reacting for 10min , add 0.18mL trifluoroacetic acid (2mmol), continue to react after 15min, add 0.2mL formaldehyde aqueous solution (37%), raise the reaction system to room temperature, add 263mg Ph 2 PC 5 h 4 N (1mmol), after stirring at room temperature for 5h, add 120mg of NH 2 CH 2 CH 2 CH...
Embodiment 3
[0045] A method for synthesizing the model substance 3 of the active center of azapropylene [iron-iron] hydrogenase containing a phosphine ligand, the chemical formula of the model substance 3 being Fe 2 [(SCH 2 ) 2 NCH 2 CH 2 CH(Me) 2 ](CO) 5 [P(C 6 h 5 ) 3 ], its chemical synthesis process is as follows:
[0046]
[0047] Under the protection condition of inert gas, add 344mg Fe in the flask equipped with stirring magnet 2 S 2 (CO) 6 (1mmol) and 30mL of tetrahydrofuran solvent to obtain a dark red solution, the resulting solution was cooled to -78°C with an acetone bath, and 2mL of lithium triethylborohydride (1M in THF) solution was slowly added under stirring conditions, and after 15min of reaction , add 0.2mL trifluoroacetic acid (2mmol), continue to react after 15min, add 0.2mL formaldehyde aqueous solution (37%), the reaction system is raised to room temperature, add 262mg of Ph 3 P (1mmol), add 105mg of NH after stirring at room temperature for 4h 2 CH ...
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![A kind of aziridine type [iron-iron] hydrogenase active center model substance containing phosphine ligand and its synthesis method](https://images-eureka.patsnap.com/patent_img/90cdb120-fa0f-484d-8cac-7340bc9fbeb3/HDA0001147688830000011.png)
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