Liquid chromatography-tandem mass spectrometry detection method for kappa-carrageenan in livestock meat

A liquid chromatography and tandem mass spectrometry technology, applied in the field of agricultural product quality and safety testing, can solve problems such as troublesome testing and expensive testing equipment, and achieve the effects of intuitive results, short time consumption, and easy popularization and application.

Inactive Publication Date: 2017-04-05
苏州市吴江区农产品检测中心 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Both of these methods require expensive detection equipment, a large amount of observation data and mathematical models need to be established, and the analysis of the experimental results requires a strong professional technical level. Therefore, the detection is troublesome.

Method used

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  • Liquid chromatography-tandem mass spectrometry detection method for kappa-carrageenan in livestock meat

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Embodiment 1

[0032] A liquid chromatography tandem mass spectrometry detection method for κ-carrageenan in livestock meat, the specific steps are as follows:

[0033] 1) Pipette the standard working solution of κ-carrageenan disaccharide alcohol (the concentration of κ-carrageenan disaccharide alcohol is 1000 mg / L), dilute it step by step with deionized water, and prepare the concentrations of 50, 100, 200, 500, 1000μg / L standard solution;

[0034] Weigh 0.1g of κ-carrageenan, dissolve it in 20ml of deionized water for more than 24 hours, and prepare κ-carrageenan additive solution;

[0035] Inject 2ml of prepared kappa-carrageenan addition solution into 20g beef sample;

[0036] 2) Crush the beef sample into minced meat, take three portions (2g each) and add them to a 50ml centrifuge tube, then add 20ml of 0.2mol / L hydrochloric acid, hydrolyze at 60°C for 16 hours, and make a sample blank at the same time; after the hydrolysis is completed, the sample hydrolyzate Natural cooling to room...

Embodiment 2

[0048] A liquid chromatography tandem mass spectrometry detection method for κ-carrageenan in livestock meat, the specific steps are as follows:

[0049] 1) Pipette the standard working solution of κ-carrageenan disaccharide alcohol (the concentration of κ-carrageenan disaccharide alcohol is 1000 mg / L), dilute it step by step with deionized water, and prepare the concentrations of 50, 100, 200, 500, 1000μg / L standard solution;

[0050] Inject 2ml of κ-carrageenan digitol sodium standard working solution (κ-carrageenan digitol concentration is 1000mg / L) in 20g pork sample;

[0051] 2) Crush the pork sample into minced meat, take three portions (2.0g each) and add them to a 50ml centrifuge tube, then add 20ml of 0.2mol / L hydrochloric acid, hydrolyze at 60°C for 16 hours, and make a sample blank at the same time; after the hydrolysis is completed, cool naturally to room temperature;

[0052] 3) The sample hydrolyzate was centrifuged at 8000rpm for 10min, and 200μl of the supern...

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Abstract

The invention discloses a liquid chromatography-tandem mass spectrometry detection method for kappa-carrageenan in livestock meat. The method comprises the following steps: preparing a standard solution; hydrolyzing a sample; extracting the material and purifying the material; and performing liquid chromatography-tandem mass spectrometry detection on the product. The method performs secondary mass fragmentation on kappa-carrageenan hydrolysate kappa-carrageenan disaccharide alcohol, characteristic fragment ion is determined, qualitative and quantitative determination can be carried out, so that the whether the sample contains the kappa-carrageenan or not can be determined. The method is used for detecting illegal addition of kappa-carrageenan in the livestock meat, compared with a near infrared spectrum detection technology and a low field nuclear magnetic resonance technology in the prior art, the detection method has the advantages of simpleness, rapidity, sensitivity, visual result, and convenient popularization, and can be used for accurately qualitative and quantitative determination for the kappa-carrageenan hydrolysate kappa-carrageenan disaccharide alcohol.

Description

technical field [0001] The invention relates to a detection method for carrageenan, in particular to a liquid chromatography tandem mass spectrometry detection method for κ-carrageenan in livestock meat, and belongs to the field of quality and safety detection of agricultural products. Background technique [0002] Carrageenan is a water-soluble sulfated galactan extracted from the cell walls of certain red algae (such as carrageen, Eucheuma, Sage, and sand vegetables), with a molecular weight of 105-106Da. Carrageenan has many characteristics such as forming hydrophilic colloid, gel, emulsification, thickening, film-forming, stabilizing, and dispersing agent. The Food Additives Committee of the World Health Organization and the Food and Agriculture Organization of the United Nations have confirmed that carrageenan is a safe additive. Therefore, carrageenan Has been widely used in food additives, daily chemical and other industries. At present, there are mainly three types ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 端礼钦陶利明王静柳敏陈枫杨广发庄丽萍
Owner 苏州市吴江区农产品检测中心
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