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Human sperm cryopreservation liquid and human sperm cryopreservation method

A technology for cryopreservation of liquid and sperm, applied in the biological field, can solve the problems of increased permeability, decreased insemination potential, increased production of reactive oxygen species, etc.

Inactive Publication Date: 2017-04-19
JINHUA VOCATIONAL TECH COLLEGE
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Problems solved by technology

[0003] Although sperm cryopreservation technology has been widely used in the fields of medical biology and animal husbandry, the integrity of sperm membrane, sperm motility and viability are reduced after sperm cryopreservation, which reduces the fertilization potential
Cryopreservation can also lead to premature capacitation of sperm, which is manifested by increased membrane permeability, increased production of reactive oxygen species, and decreased ratio of cholesterol to phospholipids, resulting in shortened sperm lifespan and reduced viability

Method used

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  • Human sperm cryopreservation liquid and human sperm cryopreservation method

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Embodiment 1

[0032] Cryopreservation, recovery and inspection of human sperm of embodiment 1

[0033] According to the "WHO Human Semen Examination and Processing Laboratory Manual" (5th edition) normal standard, 20 cases of normal sperm collection were selected. After the semen was liquefied and mixed for 30 minutes, it was divided into 2 parts (control group and experimental group), 1 mL each, and transferred into 15 mL centrifuge tubes. The control group was added with cryopreservation solution (the formula was 20% m / v SPF grade egg yolk liquid, 12% m / v glycerin, 10 μg / mL gentamycin, TES buffer (1×, pH7.5), and 1 mL was added drop by drop. The cryopreservation solution was mixed and transferred to a 2mL cryopreservation tube. The experimental group was added with an equal amount of 50μM quercetin cryopreservation solution, mixed and transferred to a cryopreservation tube. After that, the two groups were cold-balanced at 4°C for 10 minutes, - Freeze in a refrigerator at 80°C for 20 minu...

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Abstract

The invention provides a human sperm cryopreservation liquid, a human sperm cryopreservation method, and a human sperm cryopreservation and recovery method. The cryopreservation liquid comprises a yolk liquid, glycerol, antibiotics, a refrigeration buffer solution, and quercetin or derivative thereof. The human sperm cryopreservation and recovery method comprises the steps of using the human sperm cryopreservation liquid, performing cold balance at a temperature of 4 DEG C, and then performing refrigeration at a temperature of minus 80 DEG C, and next, transferring to a liquid nitrogen tank for cryopreservation; and performing unfreezing and recovering in a water bath box at a temperature of 37 DEG C before use. The sperm cryopreservation liquid, added with the quercetin at a proper concentration, can effectively protect sperms and can remarkably improve survival rate and motility of revived sperms in the subsequent sperm recovery process, so that occurrence of sperm ROS and apoptosis can be reduced; and therefore, the human sperm cryopreservation liquid has application potential in the assisted reproductive technology.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a human sperm cryopreservation solution and a human sperm cryopreservation method. Background technique [0002] According to the 2012 "China Infertility Status Survey Report", the incidence of infertility in China has increased from 3% 20 years ago to 15%, of which male factors account for 30% to 40%, and it is showing an increasing trend . From the 1990s to the present, assisted reproductive technology (ART) has become an important means to solve human fertility problems. Among them, sperm cryopreservation is an important guarantee for ART. It not only provides fertility possibilities for infertile couples with azoospermia or hereditary diseases, but also provides cancer patients who will undergo radiotherapy and chemotherapy or couples who require fertility preservation. maternity insurance. [0003] Although sperm cryopreservation technology has been widely used in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 陈浩浩郑群傅晓艳丁明星李旭升楼宏强
Owner JINHUA VOCATIONAL TECH COLLEGE
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