Agricultural antibacterial complex enzyme preparation and preparation method thereof
A compound enzyme preparation and lysozyme technology, which is applied in botany equipment and methods, fungicides, chemicals for biological control, etc., can solve the problems of difficult microbial inhibition, achieve non-toxic side effects, and good antibacterial effect , the effect of broad antibacterial spectrum
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Embodiment 1
[0022] Example 1: Compound enzyme preparation test 1
[0023] Take commercially available lysozyme, chitosanase, neutral cellulase, and β-mannanase to detect the enzyme activity, which is 1.9×10 7 U / g, 2.5×10 5 U / g, 4.7×10 4 U / g, 8.3×10 4 U / g.
[0024] Take 600mL sterile water, add CaCl 2 6g, MgCl 2 After 4 g of potassium sorbate, 1 g of potassium sorbate, and 1 g of sodium benzoate were dissolved, 143 mL of a 70% sorbitol solution was added, and the mixture was fully stirred. 10.5 g of the above-mentioned lysozyme, 60 g of chitosanase, 21.3 g of neutral cellulase, 6.0 g of β-mannanase, and 150 mL of sterile water were respectively added. Slowly adjust the pH of the enzyme solution to 4.0 with 10% hydrochloric acid, and then dilute to 1L, which is part A. Take commercially available 2×10 5 U / g neutral protease powder as part B. The combination of 1L of A enzyme solution and 1Kg of B enzyme powder is an agricultural antibacterial compound enzyme preparation.
Embodiment 2
[0025] Embodiment 2: Compound enzyme preparation test 2 and its stability investigation
[0026] Take commercially available lysozyme, chitosanase, neutral cellulase, and β-mannanase to detect the enzyme activity, which is 4.7×10 6 U / g, 2.4×10 5 U / g, 9.5×10 4 U / g, 4×10 4 U / g.
[0027] Take 6L of sterile water and add CaCl 2 60g, MgCl 2 After 40 g of potassium sorbate, 10 g of potassium sorbate, and 10 g of sodium benzoate were dissolved, 1.43 L of a 70% sorbitol solution was added and stirred well. 426 g of the above-mentioned lysozyme, 625 g of chitosanase, 105 g of neutral cellulase, 125 g of β-mannanase, and 1.5 L of sterile water were added respectively. Slowly adjust the pH of the enzyme solution to 4.0 with 10% hydrochloric acid, and then dilute to 10L, which is part A. Take commercially available 2×10 5 U / g neutral protease powder as part B. The combination of 10L of A enzyme solution and 10Kg of B enzyme powder is an agricultural antibacterial compound enzyme...
Embodiment 3
[0032] Example 3: Antibacterial activity test
[0033]Divide 600 mL of soil leachate into 4 groups, each group has 3 parallel samples, each sample is 50 mL. Group 1 is a negative control, that is, no treatment; group 2 is a positive control, sterilized at 121 °C for 30 minutes; group 3 was added with 0.5 mL of compound enzyme preparation A in Example 2, and 0.5 g of enzyme preparation was added after standing at 25 °C for 1 hour B, let stand for 1 hour after fully dissolving; group 4 was added with 0.5 mL of 5% EDTA-2Na solution, and the operation of group 3 was repeated after standing for 30 minutes. The total number of microorganisms in each group was detected by commercially available nutrient agar medium, Gao's No. 1 agar medium, and Martin-Bengal red agar medium, and the detection results are shown in Table 2.
[0034] Table 2 Experiment of soil leachate treated with compound enzyme preparation
[0035]
[0036] It can be seen from the table that the composite enzyme...
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