Preparing method of microecological preparation
A technology of micro-ecological preparations and microbial culture, applied in the field of micro-ecological preparations, can solve the problems of inappropriate preparation and processing of micro-ecological preparations, stability, poor shelf life, and poor quality of micro-ecological preparations, and improve animal immunity. , the effect of improving quality and utilization
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Embodiment 1
[0029] A method for preparing probiotics, comprising the steps of:
[0030] (1) The bacterial powders of lactic acid bacteria, Bacillus subtilis, saccharomyces, and Enterococcus faecalis were respectively prepared into liquids with a mass percentage of 3%, and 0.1 mL was inoculated on the medium respectively, and lactic acid bacteria were inoculated on the MRS liquid medium, Bacillus subtilis was inoculated on broth liquid medium, yeast was inoculated on potato dextrose liquid medium, Enterococcus faecalis was inoculated on MRS liquid medium, and cultured at 33°C for 20 hours to obtain lactic acid bacteria culture solution, Bacillus subtilis culture fluid, yeast culture fluid, Enterococcus faecalis culture fluid;
[0031] (2) Mix lactic acid bacteria culture solution, Bacillus subtilis culture solution, yeast culture solution, and Enterococcus faecalis culture solution in a volume ratio of 1.1:3.4.1:0.3:2, add bran and corn flour, and place at a temperature of Cultivate in an...
Embodiment 2
[0034] A method for preparing probiotics, comprising the steps of:
[0035] (1) The bacterial powders of lactic acid bacteria, Bacillus subtilis, saccharomyces, and Enterococcus faecalis were respectively prepared into liquids with a mass percentage of 7%, and 0.1 mL was inoculated on the medium respectively, and lactic acid bacteria were inoculated on the MRS liquid medium, Bacillus subtilis was inoculated on broth liquid medium, yeast was inoculated on potato dextrose liquid medium, Enterococcus faecalis was inoculated on MRS liquid medium, and cultured at 36°C for 23 hours to obtain lactic acid bacteria culture solution, Bacillus subtilis culture fluid, yeast culture fluid, Enterococcus faecalis culture fluid;
[0036] (2) Mix lactic acid bacteria culture solution, Bacillus subtilis culture solution, yeast culture solution, and Enterococcus faecalis culture solution in a volume ratio of 2.3:5.1:1.6:2, add bran and corn flour, and place at a temperature of 36°C Cultivate in...
Embodiment 3
[0039] A method for preparing probiotics, comprising the steps of:
[0040](1) The bacterial powders of lactic acid bacteria, Bacillus subtilis, saccharomyces, and Enterococcus faecalis are prepared into liquids with a mass percentage of 5% respectively, and 0.1 mL is inoculated on the medium respectively, and lactic acid bacteria are inoculated on the MRS liquid medium, Bacillus subtilis was inoculated on broth liquid medium, yeast was inoculated on potato dextrose liquid medium, Enterococcus faecalis was inoculated on MRS liquid medium, and cultured at 34°C for 22 hours to obtain lactic acid bacteria culture solution, Bacillus subtilis culture fluid, yeast culture fluid, Enterococcus faecalis culture fluid;
[0041] (2) Mix lactic acid bacteria culture solution, Bacillus subtilis culture solution, yeast culture solution, and Enterococcus faecalis culture solution at a volume ratio of 2:4.5:1.1:2, add bran and corn flour, and place at a temperature of 35°C Cultivate in an in...
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