Preparing method of microecological preparation

A technology of micro-ecological preparations and microbial culture, applied in the field of micro-ecological preparations, can solve the problems of inappropriate preparation and processing of micro-ecological preparations, stability, poor shelf life, and poor quality of micro-ecological preparations, and improve animal immunity. , the effect of improving quality and utilization

Active Publication Date: 2017-04-19
广西来宾八百里农业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many research technologies for microecological preparations. my country has already started research on microecological preparations as early as the 1970s, but due to inappropriate preparation and processing of microecological preparations, the microecological preparations obtained The quality of the preparation is poor, such as poor stability, shelf life, etc., and the bioavailability is also low, resulting in high cost of microecological application
[0004] In the prior art, there are reports of using β-cyclodextrin for inclusion of probiotics, but there is no report of using konjac gum for inclusion of probiotics

Method used

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Examples

Experimental program
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Embodiment 1

[0029] A method for preparing probiotics, comprising the steps of:

[0030] (1) The bacterial powders of lactic acid bacteria, Bacillus subtilis, saccharomyces, and Enterococcus faecalis were respectively prepared into liquids with a mass percentage of 3%, and 0.1 mL was inoculated on the medium respectively, and lactic acid bacteria were inoculated on the MRS liquid medium, Bacillus subtilis was inoculated on broth liquid medium, yeast was inoculated on potato dextrose liquid medium, Enterococcus faecalis was inoculated on MRS liquid medium, and cultured at 33°C for 20 hours to obtain lactic acid bacteria culture solution, Bacillus subtilis culture fluid, yeast culture fluid, Enterococcus faecalis culture fluid;

[0031] (2) Mix lactic acid bacteria culture solution, Bacillus subtilis culture solution, yeast culture solution, and Enterococcus faecalis culture solution in a volume ratio of 1.1:3.4.1:0.3:2, add bran and corn flour, and place at a temperature of Cultivate in an...

Embodiment 2

[0034] A method for preparing probiotics, comprising the steps of:

[0035] (1) The bacterial powders of lactic acid bacteria, Bacillus subtilis, saccharomyces, and Enterococcus faecalis were respectively prepared into liquids with a mass percentage of 7%, and 0.1 mL was inoculated on the medium respectively, and lactic acid bacteria were inoculated on the MRS liquid medium, Bacillus subtilis was inoculated on broth liquid medium, yeast was inoculated on potato dextrose liquid medium, Enterococcus faecalis was inoculated on MRS liquid medium, and cultured at 36°C for 23 hours to obtain lactic acid bacteria culture solution, Bacillus subtilis culture fluid, yeast culture fluid, Enterococcus faecalis culture fluid;

[0036] (2) Mix lactic acid bacteria culture solution, Bacillus subtilis culture solution, yeast culture solution, and Enterococcus faecalis culture solution in a volume ratio of 2.3:5.1:1.6:2, add bran and corn flour, and place at a temperature of 36°C Cultivate in...

Embodiment 3

[0039] A method for preparing probiotics, comprising the steps of:

[0040](1) The bacterial powders of lactic acid bacteria, Bacillus subtilis, saccharomyces, and Enterococcus faecalis are prepared into liquids with a mass percentage of 5% respectively, and 0.1 mL is inoculated on the medium respectively, and lactic acid bacteria are inoculated on the MRS liquid medium, Bacillus subtilis was inoculated on broth liquid medium, yeast was inoculated on potato dextrose liquid medium, Enterococcus faecalis was inoculated on MRS liquid medium, and cultured at 34°C for 22 hours to obtain lactic acid bacteria culture solution, Bacillus subtilis culture fluid, yeast culture fluid, Enterococcus faecalis culture fluid;

[0041] (2) Mix lactic acid bacteria culture solution, Bacillus subtilis culture solution, yeast culture solution, and Enterococcus faecalis culture solution at a volume ratio of 2:4.5:1.1:2, add bran and corn flour, and place at a temperature of 35°C Cultivate in an in...

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Abstract

The invention relates to the technical field of microecological preparations, and in particular, relates to a preparing method of a microecological preparation. Konjac gum is used as an inclusion agent; the konjac gum is hydrogel polysaccharide and is a high-molecular-weight non-ionic KGM, and has a variety of characteristics of hydrophilicity, thickening property, stability, emulsifiability, suspension property, gelling property and film-forming property; after the microecological preparation is added in a feed, the taste is improved, the quality-keeping and freshness-keeping periods of the feed are prolonged, microecological components are prevented from being oxidized and hydrolyzed, the quality of the microecological preparation is improved, the toxic and side effects are reduced, release of components of the microecological preparation is controlled, and the biological utilization rate is improved.

Description

technical field [0001] The invention relates to the technical field of probiotics, in particular to a preparation method of probiotics. Background technique [0002] The use of antibiotics has promoted the rapid development of animal husbandry; with the continuous improvement of science and living standards, the hazards of antibiotics have been generally recognized, and their long-term use in feed and medicine will lead to pathogenic bacteria in organisms The resistance to antibiotics is enhanced, and antibiotics are gradually accumulated in organisms, causing infection and secondary pollution of animal husbandry products, resulting in decreased immunity of animals, and imbalance of gastrointestinal flora in animals. health hazard. Antibiotics are currently banned or restricted in some European and American countries. [0003] Probiotics are pollution-free and residue-free green additives, which can promote animal growth, improve animal immunity, and reduce the degree of e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/18A23K20/163A23K40/30C12N1/20C12N1/16C12R1/01C12R1/125C12R1/46
CPCC12N1/16C12N1/20
Inventor 赵有宣谭春萍张艳雯
Owner 广西来宾八百里农业有限公司
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