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Composition for detection and treatment of bed bugs

A composition and bed bug technology, which can be applied in the directions of trapping or killing insect devices, animal repellents, plant growth regulators, etc., and can solve problems such as bed bug infestation

Active Publication Date: 2017-04-19
ECOLAB USA INC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a result, bedbug infestation can go on for extended periods of time without detection

Method used

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  • Composition for detection and treatment of bed bugs
  • Composition for detection and treatment of bed bugs
  • Composition for detection and treatment of bed bugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Pheromone composition from bedbug excreta was analyzed by GC / MS (Gas Chromatography / Mass Spectrometry). To obtain this sample, about 10 pieces of fecal-contaminated filter paper for colony maintenance described above were cut into 1 cm strips and soaked in 80 mL of methanol overnight to extract pheromones aggregated by bedbugs. Methanol was decanted into a 100 mL glass jar and evaporated under a gentle stream of nitrogen. After decanting, an additional 80 mL of methanol was added to the filter paper strip, and this process was repeated 3 more times. in 4mL H 2 Approximately 90 mg of the dried material was reconstituted in O and filtered through a Millex-HV, 0.45 μm PVDF filter disc (obtained from Millipore Corp. of Bedford, MA) to remove any undissolved material. The filtrate was then loaded onto a C18 Sep-Pak column (obtained from Waters, Inc. of Milford, MA) and eluted using 2 mL aliquots of increasing polarity in methanol-water, starting with 100% water and ending ...

Embodiment 2

[0082] Bedbug excreta were collected and separated into fractional groups using liquid chromatography. The effectiveness of each set of components to attract bedbugs was tested as described in Example 4. The most efficient group was further analyzed using GC / MS.

[0083] GC / MS conditions are as follows:

[0084] Column: ZB-5MS, 30m, 0.25mm, 0.25μm membrane

[0085] Initial temperature: 60°C (2min hold)

[0086] Final temperature: 320°C (10min hold)

[0087] Heating rate: 20°C / min

[0088] Injection: 2 μl

[0089] Syringe: PTV splitless

[0090] Scanning: 50-750amu

[0091] Samples were analyzed without dilution, both as such and derivatized with BSTFA (bistrimethylsilyltrifluoroacetamide).

[0092] Figure 2A Results from initial separations are shown, with groups of components indicated as 1-3. exist Figure 2B (sample injected as received), 2C (in Figure 2B Further isolated group 3 is shown in 2D (derivatized sample, 5-9 min), and 2E (derivatized sample, 8.5-14 ...

Embodiment 3

[0097] The extract obtained in Example 1 was tested for its ability to increase the efficacy of insecticides and to increase the time spent on insecticide-treated surfaces. Bedbugs generally avoid resident on insecticide-treated surfaces. It is hypothesized that the extract would keep the bugs on the surface longer.

[0098] Experimental field setup: A circular field with a diameter of 20 cm as previously described by Olson et al. (2009) was used for the analysis. Each field contained two 25mm glass microfiber filter discs fixed to the field approximately 10 cm apart ( Grade GF / A, obtained from GE Healthcare in Piscataway, NJ). In each test round, one disc was treated and the other was left untreated. Treated discs were treated with insecticide or insecticide+extract.

[0099] To measure the time spent on the treated surface, a bedbug was introduced into the field and its movement was recorded over a 4-hour period. Calculate the total amount of time spent on / off the trea...

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PUM

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Abstract

The present disclosure provides for a composition and method for attracting bed bugs to a trap or to a location where an insecticide or pesticide is present, or to a trap or a location where a bed bug infestation can be detected. The present disclosure provides for a composition for attracting bed bugs that includes one or more of methyl diethanolamine (MDEA), monoethanolamine (MEA), diethanolamine (DEA), triethanolamine (TEA), and dimethyl trisulfide (DMTS); a carrier; and optionally one or more amides selected from oleamide, octanamide, nonanamide, and laurylamide; urea, biuret, or triuret; and one or more aliphatic fatty acids. The present disclosure further provides for a method for treating an article by applying the composition to the article, where the article comprises a device for trapping or detecting bed bugs or a device for treating a bed bug infestation.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of Provisional Application Serial No. 62 / 014,534, filed June 19, 2014, and Provisional Application Serial No. 62 / 110,924, filed February 2, 2015, each of which is incorporated herein by reference in its entirety. technical field [0003] The present disclosure relates generally to the field of pest control, including bedbugs. The present disclosure includes compositions and methods for applying a product to a target and killing bedbugs. Background technique [0004] Bedbugs are small parasitic insects that feed exclusively on the blood of animals, especially humans. A common bedbug, the temperate bedbug (Cimex Lectularius), is adapted to live with humans and preferably feeds on human blood. Bedbugs have lived with humans since time immemorial, although many people living in the United States have never seen a bedbug. In recent decades, however, increased international travel has c...

Claims

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Application Information

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IPC IPC(8): A01N33/02A01N31/00
CPCA01M1/023A01M1/026A01M1/02A01M1/2011A01M1/223A01M1/103A01M1/14A01N33/08A01N41/12Y02A50/30A01N51/00A01N53/00
Inventor J·F·奥尔森S·A·柯勒斯L·M·威尔维尔斯R·穆恩Y·基利恩
Owner ECOLAB USA INC
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