Application of Monobenzone to preparation of medicaments

A technology for monobenzone and medicine preparation, which is applied in the field of use of monobenzone in medicine preparation, can solve the problems such as the need for further development of monobenzone

Inactive Publication Date: 2017-04-26
WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, the use of monobenzone in the preparation of medicines also needs to be further developed

Method used

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  • Application of Monobenzone to preparation of medicaments
  • Application of Monobenzone to preparation of medicaments
  • Application of Monobenzone to preparation of medicaments

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1 Anti-KSHV lytic phase replication activity detection of monobenzone

[0050] 1. Experimental materials

[0051] 1.1 Cells and viruses

[0052] iSLK.219 cells were donated by Professor Dr. Don Ganem. The cells contain recombinant virus rKSHV.219 and an RTA expression system regulated by doxycycline. In this cell, the RTA expression sequence was constructed into the pRetro-XTet-ON inducible expression system (Clontech, Mountainview, CA), and the expression of this system was regulated by doxycycline. In the presence of doxycycline, RTA expression takes place. 293T cells were preserved in our laboratory and purchased from the American Type Culture Collection (ATCC).

[0053] Monobenzone was purchased from Biochempartner.

[0054] 1.2 Reagents

[0055] DMEM medium, 1640 medium and FBS were purchased from GIBCO; Alamarblue activity detection kit was purchased from Promega; SYBR mixture (iTaq TM Universal Green Supermix) was purchased from Bio-Rad Company. ...

Embodiment 2

[0111] Example 2 Activity Detection of Monobenzone Anti-EBV Lysis Phase Replication

[0112] 1. Experimental materials

[0113] 1.1 Cells and viruses

[0114]HONE-1 cells were donated by Dr.George Tsao, and contain the recombinant virus BAC-EBV strain, which contains the complete sequence of the EBV genome. Co-induction with 20 ng / mL TPA and 0.3 mM sodium butyrate (NaB) can make the virus enter the lytic phase and replicate.

[0115] 1.2 Reagents

[0116] 1640 medium and FBS were purchased from GIBCO; Alamarblue activity detection kit was purchased from Promega; SYBR mixture (iTaq TM Universal Green Supermix) was purchased from Bio-Rad Company.

[0117] 1.3 Experimental Instruments

[0118] The multi-label microplate reader was purchased from PerkinElmer; the fluorescent quantitative PCR instrument (Bio-Rad CFX96 Touch TM Real-Time PCR detection system) was purchased from Bio-Rad Company; 1.0R refrigerated centrifuge and cell culture box were purchased from Thermo C...

Embodiment 3

[0138] Embodiment 3: the detection of monobenzone anti-HSV-1 and HSV-2 activity

[0139] 1. Experimental materials

[0140] 1.1 Cells and viruses

[0141] Vero cells were preserved in our laboratory and purchased from the American Type Culture Collection (ATCC). HSV-1 is an F strain, which is preserved by the Wuhan Institute of Virology, Chinese Academy of Sciences. HSV-2 is a G strain, which is preserved by the Wuhan Institute of Virology, Chinese Academy of Sciences.

[0142] 1.2 Reagents

[0143] DMEM medium and FBS were purchased from GIBCO; Alamarblue activity detection kit was purchased from Promega; crystal violet staining solution.

[0144] 1.3 Experimental Instruments

[0145] The multi-label microplate reader and the high-content cell analyzer were purchased from PerkinElmer; the cell incubator was a product of Thermo.

[0146] 2. Experimental methods and results

[0147] 2.1 Cytotoxicity detection of monobenzone on Vero cells.

[0148] (1) Divide Vero cells ...

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Abstract

The invention provides an application of Monobenzone to preparation of medicaments, and the medicament is used for treating or preventing Herpesviridae. According to the embodiment, Monobenzone can be effectively applied to treatment or prevention of infection of herpes virus.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular, the invention relates to the use of monobenzone in the preparation of medicines. Background technique [0002] Herpesviridae is a family of viruses with envelopes and double-stranded DNA genomes. Members of this family can widely infect animals and humans and induce corresponding diseases. There are currently eight herpes viruses that can infect humans, mainly including: Herpes simplex virus-1 (HSV-1), Herpes simplex virus-2 (HSV-2), Varicella zoster virus (VZV), Epstein-Barr virus (EBV), cytomegalovirus (CMV), human herpes virus type VI (HHV-6), human herpes virus type VII (HHV-7) and Kaposi's sarcoma-associated herpesvirus (KSHV). According to the differences in genome sequence, structure and physical and chemical properties, Herpesviridae can be divided into three subfamilies: α-Herpesvirinae, β-Herpesvirinae and γ-Herpesvirinae. The life cycle of herpes virus is divided into typi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/085A61P31/22
CPCA61K31/085
Inventor 陈绪林陈军刚
Owner WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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