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Method for preparing slow-release sea cucumber bait from fish byproducts and immobilized enzymes

A technology of immobilizing enzymes and leftovers, applied in the field of bioengineering, can solve problems such as indigestion and utilization

Active Publication Date: 2017-05-10
SHANDONG HOMEY AQUATIC DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the deficiency in the prior art that sea cucumbers cannot be digested and utilized by directly feeding fish leftovers, the present invention provides a slow-release food prepared by using fish leftovers and immobilized enzymes, which has a reasonable process and high intestinal utilization rate of sea cucumber bait. The method of sea cucumber bait

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The method for preparing slow-release sea cucumber bait by using fish waste and immobilized enzymes, it goes through the following process steps:

[0024] (a) Fermentation treatment of fish waste

[0025] (a1) Cultivation of fermented seed solution

[0026] Insert the W2 strain into the DAE medium, control the temperature at 22°C, and cultivate under the condition of 160r / min for 3 days to obtain the seed bacterial liquid. The OD600 of the seed bacterial liquid is 1.1; the formula of the DAE medium is: peptone 0.5 g, 0.1g of yeast extract, 0.015g of iron phosphate, and 95ml of seawater; the W2 strain is a protease-producing strain isolated and screened from the fish intestine, and the inoculation ratio of the W2 strain to the DAE medium is 1:400;

[0027] (a2) Preparation of fish waste fermentation broth

[0028] Selecting fish scraps as raw materials, feeding them into a grinder and grinding them into a minced meat shape; weighing the minced fish scraps and putting t...

Embodiment 2

[0040] The method for preparing slow-release sea cucumber bait by using fish waste and immobilized enzyme is characterized in that: it goes through the following process steps:

[0041] (a) Fermentation treatment of fish waste

[0042] (a1) Cultivation of fermented seed solution

[0043] Insert the W2 strain into the DAE medium, control the temperature at 25°C, and cultivate it under the condition of 150r / min for 2 days to obtain the seed bacterial liquid; the OD600 of the seed bacterial liquid is 1.2; the formula of the DAE medium is: peptone 0.8 g, 0.05g of yeast extract, 0.02g of iron phosphate, and 100ml of seawater; the W2 strain is a protease-producing strain isolated and screened from the fish intestine, and the inoculation ratio of the W2 strain to the DAE medium is 1:500;

[0044] (a2) Preparation of fish waste fermentation liquid

[0045]Selecting fish scraps as raw materials, feeding them into a grinder and grinding them into a minced meat shape; weighing the minc...

Embodiment 3

[0057] The method for preparing slow-release sea cucumber bait by using fish waste and immobilized enzyme is characterized in that: it goes through the following process steps:

[0058] (a) Fermentation treatment of fish waste

[0059] (a1) Cultivation of fermented seed solution

[0060] Insert the W2 strain into the DAE medium, control the temperature at 20°C, and cultivate under the condition of 180r / min for 3 days to obtain the seed bacterial liquid; the OD600 of the seed bacterial liquid is 1.0; the formula of the DAE medium is: peptone 0.4 g, 0.2g of yeast extract, 0.01g of ferric phosphate, and 90ml of seawater; the W2 strain is a protease-producing strain isolated and screened from the fish intestine, and the inoculation ratio of the W2 strain to the DAE medium is 1:300;

[0061] (a2) Preparation of fish waste fermentation broth

[0062] Selecting fish scraps as raw materials, feeding them into a grinder and grinding them into a minced meat shape; weighing the minced ...

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Abstract

The invention discloses a method for preparing slow-release sea cucumber bait from fish byproducts and immobilized enzymes, and solves the problem that the existing fish byproducts cannot be digested and utilized when being directly fed to sea cucumbers. The method comprises the following steps of inoculating bacteria produced bacterial strains formed by separating and screening in a fish intestinal tract in a culture medium, so as to culture a seed bacteria solution; putting fish byproduct meat paste into the seed bacteria solution, so as to be fermented to form a fermented liquid; injecting a chitosan solution into a sodium hydroxide and methyl alcohol mixed solution, so as to coagulate to form a hollow spherical chitosan carrier; putting the carrier into a glutaraldehyde aqueous solution, so as to be activated; enwrapping enzymes into the hollow spherical chitosan carrier, so as to form the immobilized enzymes; and delivering the immobilized enzymes and fermented liquid to a holothurian culture water area. The method is reasonable in technology and feasible to operate. The stability of immobilized enzymes is high, the half decay period is long, enzymolysis of the fish byproduct fermented liquid is slow, sea cucumber bait is released, and moreover, deposited residual bait is degraded, the bait casting frequency is reduced, and the labor cost is reduced.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a method for preparing slow-release sea cucumber bait by using fish leftovers and immobilized enzymes. Background technique [0002] We know that sea cucumber has high nutritional value and medicinal value, and is regarded as an important sea treasure. In recent years, the increasing demand for sea cucumber consumption has led to the overexploitation of natural resources and a sharp decline in the population of sea cucumbers. Therefore, artificial breeding of sea cucumbers has also developed. The bait is the material basis for the growth and development of sea cucumbers. With the development of large-scale cultivation of sea cucumbers, natural bait can no longer meet the needs of sea cucumber cultivation, so artificial feeding of bait is required. At present, artificial feeding of bait has problems such as high labor cost, low utilization rate of bait, and difficulty in g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/80A23K10/26A23K10/12C12N11/10C12N11/04
CPCC12N9/50C12N11/04C12N11/10
Inventor 孙永军毛旭华鞠文明唐晓波胡炜
Owner SHANDONG HOMEY AQUATIC DEV
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