A kind of in vivo drug resistance induction model of Pseudomonas aeruginosa and its construction method and application

A technology of Pseudomonas aeruginosa and a construction method, which is applied to the in vivo drug resistance induction model of Pseudomonas aeruginosa animals and the field of its construction, and can solve the problem of not being able to simulate the development law of Pseudomonas aeruginosa drug resistance and model evaluation. Inaccurate standards, lack of Pseudomonas aeruginosa models, etc.

Active Publication Date: 2019-12-10
BEIJING CHINESE MEDICINE HOSPITAL AFFILIATED CAPITAL MEDICAL UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Therefore, the technical problem to be solved by the present invention lies in the lack of Pseudomonas aeruginosa respiratory tract infection animal models in the prior art, which cannot simulate the development of Pseudomonas aeruginosa drug resistance in the airway of the body, and the model evaluation criteria are not accurate defects, thereby providing a PA animal in vivo drug resistance induction model and its construction method and application

Method used

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  • A kind of in vivo drug resistance induction model of Pseudomonas aeruginosa and its construction method and application
  • A kind of in vivo drug resistance induction model of Pseudomonas aeruginosa and its construction method and application
  • A kind of in vivo drug resistance induction model of Pseudomonas aeruginosa and its construction method and application

Examples

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Embodiment 1

[0072] This embodiment provides a method for constructing a drug resistance induction model in PA animals, comprising the following steps:

[0073](1) Take 200 healthy ICR mice, half male and half female, weighing 14±1g, and randomly group them into groups. The induction modeling period is set to 3 days, 5 days, 7 days, and 9 days respectively, and a blank is set for each induction modeling period. Control group, non-induced drug resistance model group A1, non-induced drug resistance model administration group A2, induced drug resistance model group B1 and induced drug resistance model administration group B2, a total of 20 groups (see Table 2 below), each group 10, spare; the non-induced drug resistance model group A1 and the non-induced drug resistance model administration group A2 are collectively referred to as group A, and the induced drug resistance model group B1 and the induced drug resistance model administration group B2 are collectively referred to as Group B;

[0...

Embodiment 2

[0101] In this example, on the basis of Example 1, the expression level of the efflux pump gene mexC in the mouse lung tissue is used to verify whether the drug resistance induction model in the PA animal is successfully constructed. The method for expressing the amount of mexC gene comprises the steps:

[0102] 1) Collect the lung tissue of each group of mice described in Example 1 as a sample and place it in a mortar, add liquid nitrogen, grind it finely, collect it in a 1.5ml centrifuge tube, add 1ml Trizol to each sample, and extract RNA; Flick the bottom of the EP tube with 1ml TrizolReagent to resuspend the mixed sample, place at room temperature for 20min, 4°C, 12000rpm, centrifuge for 10min; transfer the clarified supernatant into a new EP tube, add 0.2ml chloroform, and cap tightly Cover the tube, shake vigorously for 15s, incubate at room temperature for 2-3min, 4°C, 12000rpm, centrifuge for 15min; transfer the supernatant to a new EP tube, add 0.5ml isopropanol, mix...

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Abstract

The invention discloses a model for inducing drug resistance in a pseudomonas aeruginosa animal as well as a construction method and an application of the model. With the application of the model constructed by the method, better simulation of a phenomenon of PA infection in respiratory tract of a patient who accepts wide-spectrum antibiotics clinically for a long time can be achieved, and development and change rule of the drug resistance of pseudomonas aeruginosa in a mouse body can be observed, so as to simulate the change of the bacterium (the pseudomonas aeruginosa) in the body and to facilitate study on antibacterial drug-resistance medicine treatment; and meanwhile, the animal model, as a lung index evaluating animal model, is more intuitive, accurate in evaluating result, short in construction cycle and high in efficiency.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a drug resistance induction model of Pseudomonas aeruginosa (PA) in animals and a construction method and application thereof. Background technique [0002] Pseudomonas aeruginosa (PA), formerly known as Pseudomonas aeruginosa, is a non-fermenting gram-negative bacillus that widely exists in the skin, respiratory tract and intestinal tract of normal people and in hospital environments. PA infection can cause a variety of diseases, such as burns, sepsis, intestinal infection, suppurative infection of postoperative wounds, and respiratory tract infection. For patients with basic diseases and long-term use of antibiotics and antineoplastic drugs, PA infection pneumonia is more likely to occur due to their weak immune system or impaired immune function. [0003] The infection of PA is usually treated with antibiotics, but with the extensive use of antibiotics, PA is very pro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/00A61K35/74A61K31/5383A01K67/02
CPCA01K67/02A61K31/5383A61K35/74A61K49/0008
Inventor 刘清泉崔晓兰崔煦然郭玉红赵京霞丁军颖
Owner BEIJING CHINESE MEDICINE HOSPITAL AFFILIATED CAPITAL MEDICAL UNIV
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