Method for improving sludge settleability and removing organic matter by using filamentous fungi

A filamentous fungus and sludge settling technology, which is applied in microorganism-based methods, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problem of high cost, difficult mass production, limited flocculant output, and high extraction of microbial flocculants. cost, etc.

Inactive Publication Date: 2017-05-10
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Microbial flocculants make up for the shortcomings of traditional methods in terms of environmental safety, but they still have certain limitations. The existing problems are as follows: (1) Microorganisms mostly grow under pure medium composed of glucose, sucrose, yeast extract, peptone, etc. And produce flocculant, the cost is high and it is difficult to produce in batches, and the extraction of microbial flocculant also requires high cost; (2) flocculant production: the production of flocculant produced by microorganisms in the growth process is limited

Method used

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  • Method for improving sludge settleability and removing organic matter by using filamentous fungi
  • Method for improving sludge settleability and removing organic matter by using filamentous fungi
  • Method for improving sludge settleability and removing organic matter by using filamentous fungi

Examples

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Embodiment 1

[0020] A method for improving sludge settling performance and removing organic matter by using filamentous fungi, specifically as follows:

[0021] After the pure culture of Talaromyces sp. forms larger colonies on PDA (potato-dextrose-agar medium), rinse the medium with sterile distilled water, and dilute the rinse with sterile distilled water to make a spore suspension , the concentration measured by hemocytometer was 2.0×10 6 individual / mL; with the 1% inoculum of the sludge volume, the spore suspension is directly inoculated into the sterilized sludge of 30g / L with TS (dry matter content), and the PDB culture solution (potato- Glucose culture solution), the temperature was maintained at 30° C., the rotation speed of the shaker was 140 rpm, and the pH was 6.81.

[0022] After using the above method to process for 5 days, the sludge can form uniform mycelium balls under the action of filamentous fungi, and this treatment method greatly improves the sedimentation performance...

Embodiment 2

[0026] A method for improving sludge settling performance and removing organic matter by using filamentous fungi, specifically as follows:

[0027] After the pure culture of Talaromyces sp. forms larger colonies on PDA (potato-dextrose-agar medium), rinse the medium with sterile distilled water, and dilute the rinse with sterile distilled water to make a spore suspension , the concentration measured by hemocytometer is: 1.0×10 6 individual / mL; with 1% inoculum of the sludge volume, the spore suspension is directly inoculated into TS (dry matter content) in the sterilized sludge of 23g / L, and the PDB culture solution of 15% of the sludge volume is added, at pH Incubate under 7.9. The temperature is kept at 35°C and the rotating speed of the shaker is 150rpm; during the cultivation process, the sludge can also form mycelial balls under the action of filamentous fungi. After 7 days of treatment, the sedimentation performance of the sludge has been improved to a certain extent, a...

Embodiment 3

[0030] A method for improving sludge settling performance and removing organic matter by using filamentous fungi, specifically as follows:

[0031] After the pure culture of Talaromyces sp. forms larger colonies on PDA (potato-dextrose-agar medium), rinse the medium with sterile distilled water, and dilute the rinse with sterile distilled water to make a spore suspension , the concentration measured by hemocytometer is: 5.0×10 6 Individuals / mL; Inoculate the spore suspension directly into the sterilized sludge with an inoculation amount of 1% of the sludge volume, wherein the sludge TS is 10g / L, add PDB culture solution with 20% of the sludge volume, and the pH is 5.8 The temperature was maintained at 25 °C and the rotation speed of the shaker was 160 rpm.

[0032] In the cultivation process, the mycelial balls that the sludge can also form under the action of filamentous fungi, after 7 days of treatment, the sedimentation performance of the sludge has been improved to a cert...

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Abstract

The invention belongs to the field of municipal sludge disposal and treatment and particularly relates to a method for improving sludge settleability and removing organic matter by using filamentous fungi. A spore suspension is prepared from the filamentous fungi, sludge in a concentration tank is directly inoculated with the spore suspension for culture, the concentration of the spore suspension is 1.0*10<6>/mL-5.0*10<6>/mL, the inoculum size of the spore suspension is 1% of the volume of the sludge, the dry matter content of the sludge is 10-30 g/L, solid sludge particles are captured by mycelia of the filamentous fungi and compressed to form mycelium pellets after the filamentous fungi are cultured in the sludge, the original pore structure of the sludge is changed, free water is released, and a bioseparation effect is realized, so that the sludge settleability can be improved and the organic matter in the sludge can be removed simultaneously.

Description

technical field [0001] The invention belongs to the field of urban sludge treatment and disposal, and in particular relates to a method for improving sludge settling performance and removing organic matter by using filamentous fungi. Background technique [0002] At present, the treatment and disposal of municipal sludge has attracted widespread attention. Sludge treatment methods mainly include sludge concentration, stabilization, and composting; sludge disposal methods mainly include incineration, sanitary landfill, and land use. Composting, incineration, landfill, land use and other methods all have requirements on the moisture content of sludge. Sludge containing more than 96% of moisture is still fluid and does not use subsequent treatment. When the moisture content of sludge is below 50%, heat When the value is above 5000kJ / kg, the sludge can support combustion. Therefore, sludge dehydration is an important link. Dehydration reduces the volume and weight of sludge, w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F11/02C12N1/14C02F101/30C12R1/645
CPCC02F11/02C02F11/008C02F2101/30C12N1/14
Inventor 刘和符波史佳晟徐晓宇刘宏波
Owner JIANGNAN UNIV
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