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Abalone muscle polysaccharose and preparation method and application thereof

A muscle and polysaccharide technology, applied in the field of polysaccharides, achieves the effect of good breast cancer and liver cancer cell growth and reproduction, easy synthesis, and inhibition of breast cancer and liver cancer cell growth and reproduction

Inactive Publication Date: 2017-05-10
JIMEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the structure and function of abalone muscle polysaccharides

Method used

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  • Abalone muscle polysaccharose and preparation method and application thereof
  • Abalone muscle polysaccharose and preparation method and application thereof
  • Abalone muscle polysaccharose and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1. After removing surface impurities, 1kg of abalone muscle was mixed with 4L of cold water and crushed, then stirred and extracted at 10°C for 2h, and centrifuged at 5000rpm for 15min to remove insoluble matter to obtain 4.5L of supernatant.

[0038] 2. Adjust the pH of the supernatant obtained in step 1 to 9.0 with sodium hydroxide, then add 4.5 g of alkaline protease and 4.5 g of collagenase successively, and perform enzymatic hydrolysis at 50° C. for 2 hours.

[0039] 3. Adjust the pH of the supernatant obtained in step 2 to 6.5 with hydrochloric acid, then add 4.5 g of neutral protease and 4.5 g of papain, and perform enzymatic hydrolysis at 50° C. for 2 hours.

[0040] 4. After enzymatic hydrolysis, concentrate to 0.5L by rotary evaporation, add 0.33L absolute alcohol to the concentrated solution, let it stand at room temperature for 30min, and filter to remove the precipitate.

[0041] 5. Continue to slowly add 1.67L of absolute alcohol to the filtrate obtained i...

Embodiment 2

[0045] 1. After removing surface impurities, 1kg of abalone muscle was mixed with 2L of cold water and crushed, then stirred and extracted in a 20°C incubator for 30min, and centrifuged at 5000rpm for 30min to remove insoluble matter to obtain 2.5L of supernatant.

[0046] 2. Adjust the pH of the supernatant obtained in step 1 to 9.0 with sodium hydroxide, then add 5.0 g of alkaline protease and 5.0 g of collagenase successively, and perform enzymatic hydrolysis at 45° C. for 1 h.

[0047] 3. Adjust the pH of the supernatant obtained in step 2 to 6.0 with hydrochloric acid, then add 5.0 g of neutral protease and 5.0 g of papain, and perform enzymatic hydrolysis at 45° C. for 1 h.

[0048] 4. After enzymatic hydrolysis, concentrate to 0.4L by rotary evaporation, add 0.1L absolute alcohol to the concentrated solution, let it stand at room temperature for 60min, and filter to remove the precipitate.

[0049] 5. Continue to slowly add 1.5L of absolute alcohol to the filtrate obtai...

Embodiment 3

[0053] 1. After removing surface impurities, 1kg of abalone muscle was mixed with 3L of cold water and crushed, then stirred and extracted in a 30°C incubator for 30min, and centrifuged at 5000rpm for 25min to remove insoluble matter to obtain 3.5L of supernatant.

[0054] 2. Adjust the pH of the supernatant obtained in step 1 to 9.0 with sodium hydroxide, then add 1.75 g of alkaline protease and 1.75 g of collagenase successively, and perform enzymatic hydrolysis at 55° C. for 3 hours.

[0055] 3. Adjust the pH of the supernatant obtained in step 2 to 7.0 with hydrochloric acid, then add 1.75 g of neutral protease and 1.75 g of papain, and perform enzymatic hydrolysis at 55° C. for 3 hours.

[0056]4. After enzymatic hydrolysis, concentrate to 0.3L by rotary evaporation, add 0.13L absolute alcohol to the concentrated solution, let it stand at room temperature for 60min, and filter to remove the precipitate.

[0057] 5. Continue to slowly add 0.77L of absolute alcohol to the f...

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Abstract

The invention discloses abalone muscle polysaccharose and a preparation method and application thereof. The polysaccharose mainly consists of glucose and mannose, the molecular weight is 3200 Da, the main chain of the polysaccharose is composed of glucoses through first to fourth glucosidic bonds and first to sixth glucosidic bonds, the side chain of the polysaccharose is composed of first to sixth glucosidic bonds, and the saccharose residue is in alpha configuration. The abalone muscle polysaccharose extracted in the invention has a good effect of inhibiting the activity of cell growth and propagation of breast cancer and liver cancer, and plays a diversified comprehensive utilization role in product development of abalone.

Description

[0001] 【Technical field】 [0002] The invention relates to a polysaccharide, in particular to a novel polysaccharide extracted from abalone muscle and its preparation process and application. [0003] 【Background technique】 [0004] Abalone is known as marine ginseng, and its nutrients and bioactive components such as protein and polysaccharide help to promote human health. The extraction of polysaccharides from abalone has attracted extensive attention in recent years due to their good anti-oxidation, enhanced immune regulation, anti-tumor and anti-coagulant activities. Chinese Patent Publication No. CN102898538 A discloses an acidic polysaccharide in abalone viscera and health products containing it and its application. functional factor. However, there are few reports on the structure and function of abalone muscle polysaccharides. [0005] 【Content of invention】 [0006] The object of the present invention is to provide an abalone muscle polysaccharide, which is extract...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P35/00
CPCC08B37/0003A61K31/715C08B37/0009
Inventor 翁武银王军玲陈俊
Owner JIMEI UNIV
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